Use of Anti-Rh Sera for Demonstrating Agglutination Activating Factor in Rheumatoid Arthritis.

Waller, Marion; Vaughan, John H.

doi:10.3181/00379727-92-22426

Cited by 113 publications

(40 citation statements)

References 1 publication

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“…In general, R F were hardly ever found by any anti-D sera except anti-CD Ripley, which uncovered these antibodies in as many as 75% of seropositive RA patients. This observation confirms earlier findings by Waller et a1 (8,20,21).…”

Section: Incidence and Titers Of Cda In The Sera Of Healthy Subjects supporting

confidence: 83%

“…Purified IgG from 12 different types of anti-D sera was used to produce Fab, fragments by digestion with acid lysosomal protease. Three of these sera, especially Waller's anti-CD Ripley (20), proved suitable for determining CDA. These purified Fab, fragments had a molecular weight of 100,000 and, on immunodiffusion, were precipitated by commercial antiFab but not by anti-Fc, as reported earlier (4).…”

Section: Resultsmentioning

confidence: 99%

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Cathepsin D Agglutinators in Rheumatoid Arthritis

Artmann

Fehr

Böni

1977

Arthritis & Rheumatism

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The incidence and titer of cathepsin D agglutinators (CDA) were significantly higher in seropositive rheumatoid arthritis (RA) sera than in the sera of healthy blood donors or of patients with other rheumatic diseases, including seronegative RA. A significant elevation was also found in synovial fluid (SF) samples from seropositive RA patients. CDA in the SF tended to be increased when compared with the corresponding serum. The levels of CDA correlated positively with those of rheumatoid factors (RF) when the latter were determined with IgG anti‐CD Ripley‐coated erythrocytes, but not when they were determined by the Waaler‐Rose or latex tests. The increased CDA titers do not seem to be the result of significant amounts of IgM agglutinators or of the presence of IgM‐RF. These findings suggest the existence of a link between the formation of RF and CDA, but the nature of this link cannot be fully explained.

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Section: Incidence and Titers Of Cda In The Sera Of Healthy Subjects supporting

confidence: 83%

Section: Resultsmentioning

confidence: 99%

Cathepsin D Agglutinators in Rheumatoid Arthritis

Artmann

Fehr

Böni

1977

Arthritis & Rheumatism

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“…The Ripley anti-CD antibody (19) These observations have been taken to mean: (a) only a minority of the RF-PFC have enough stored RF for plaque formation in the absence of continued protein synthesis; (b) the cyclic nucleotides probably do not modulate significantly the synthesis or release of the RF; and (c) the release depends upon intact microtubular or membrane structures.…”

Section: Resultsmentioning

confidence: 99%

Rheumatoid factor-producing cells detected by direct hemolytic plaque assay.

Vaughan¹,

Chihara²,

Moore³

et al. 1976

J. Clin. Invest.

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“…This is visible in the lower portion of Figure 2, sedimenting slightly faster than the 19S component visible in the upper frames after dissociation in acid buffer. At least five examinations of whole serum Do at different dilutions always showed the rapidly sedimenting peak with an s-rate higher than the usual 21 to 23S for the rheumatoid factor. However, on the addition of 7S y-globulin to the whole serum, as described in the preceding paragraph, this rapidly sedimenting peak showed a shift in s-rate to close to 22S with a definite loss of area.…”

Section: Resultsmentioning

confidence: 99%

Gamma Globulin Complexes in Rheumatoid Arthritis and Certain Other Conditions

Kunkel¹,

Müller‐Eberhard²,

Fudenberg³

et al. 1961

J. Clin. Invest.

275

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Recent studies have demonstrated that the factors responsible for the various serological reactions currently employed for the diagnosis of rheumatoid arthritis represent macroglobulins which, in the isolated state, have a corrected sedimentation coefficient of approximately 19S (1-5). In serum and plasma, these proteins exist as complexes with low molecular weight y-globtulins and show a higher sedimentation rate, approximately 22S (6, 7). It has become clear that the rheumatoid factors have a specific affinity for ordinary y-globulin showing a behavior, particularly in certain of the serological tests, like that of anti-y-globulin antibodies. The possibility has been raised that the rheumatoid factor complex in serum represents an antigen-antibody complex.During the course of investigation of the rheumatoid factors and their complexes through ultracentrifugal analyses of sera showing high titer reactions in the latex fixation test and y-globulin precipitin test, another group of y-globulin complexes was encountered. These had sedimentation rates between 9 and 17S and were dissociated in urea and acid buffers in a manner similar to the previously observed dissociation of the rheumatoid factor complexes. Again the question arises as to whether these are antigen-antibody comlplexes or simple aggregates of y-globulin.Gamma globulin components in the 9 to 12S range have been observed in many preparations of normal Fr II y-globulin. The (15,16), although the sedimentation rate of the main abnormal protein was lower than the 18 to 20S range of the proteins described by Waldenstr6m. Macroglobulins of the latter class are a homogeneous group and are known to be stable proteins which do not dissociate in urea and acid and alkaline buffers (17). They have specific immunological properties and dissociate in the presence of agents breaking disulfide bonds (18).In the present study, sera from 11 different patients, 7 with classical rheumatoid arthritis, were found to contain multiple y-globulin complexes. These were readily detected in whole serum and plasma by direct ultracentrifugal analysis. They did not possess rheumatoid factor activity but were associated in the same sera with large amounts of rheumatoid factor. Investigation of the association and dissociation reactions of these complexes was carried out and certain of their properties de-fined. Evidence for and against the hypothesis that they represent antigen-antibody complexes of the y-globlulin-anti-y-glol)bulin type was sought. MATERIAL AND MIIETHODSSix of the 11 patients whose sera were specifically analyzed in this study were observed in the clinic and outpatient department of the Rockefeller Hospital. Three of the remainder represented patients in various New York 1-17

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Use of Anti-Rh Sera for Demonstrating Agglutination Activating Factor in Rheumatoid Arthritis.

Cited by 113 publications

References 1 publication

Cathepsin D Agglutinators in Rheumatoid Arthritis

Cathepsin D Agglutinators in Rheumatoid Arthritis

Rheumatoid factor-producing cells detected by direct hemolytic plaque assay.

Gamma Globulin Complexes in Rheumatoid Arthritis and Certain Other Conditions

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