2013
DOI: 10.1128/aem.01029-13
|View full text |Cite
|
Sign up to set email alerts
|

Use of Antibody Responses against Locus of Enterocyte Effacement (LEE)-Encoded Antigens To Monitor Enterohemorrhagic Escherichia coli Infections on Cattle Farms

Abstract: dEnterohemorrhagic Escherichia coli (EHEC) is a significant zoonotic pathogen causing severe disease associated with watery and bloody diarrhea, hemorrhagic colitis, and the hemolytic-uremic syndrome (HUS) in humans. Infections are frequently associated with contact with EHEC-contaminated ruminant feces. Both natural and experimental infection of cattle induces serum antibodies against the LEE-encoded proteins intimin, EspA, EspB, and Tir and the Shiga toxins Stx1 and Stx2, although the latter are poorly immun… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

0
6
0
1

Year Published

2016
2016
2022
2022

Publication Types

Select...
4
1

Relationship

0
5

Authors

Journals

citations
Cited by 5 publications
(7 citation statements)
references
References 32 publications
0
6
0
1
Order By: Relevance
“…On the other hand, a humoral response against EspB has been observed after vaccination, as mentioned previously, and in cattle after natural [21, 27, 52] as well as experimental infections [27, 29, 53] and in human patients suffering from HUS [53], although it is less antigenic than Intimin.…”
Section: Discussionmentioning
confidence: 80%
See 1 more Smart Citation
“…On the other hand, a humoral response against EspB has been observed after vaccination, as mentioned previously, and in cattle after natural [21, 27, 52] as well as experimental infections [27, 29, 53] and in human patients suffering from HUS [53], although it is less antigenic than Intimin.…”
Section: Discussionmentioning
confidence: 80%
“…Furthermore, Intimin has strong antigenic determinants and it has been shown to be a target of humoral immune responses in different host species and animal models such as humans [47, 48], cattle [21, 29, 32, 34, 35], rabbits [49], pigs [50] and mice [51]. Antibodies against this protein have also been described after experimental (Bretschneider et al[20] as well as natural infections [21, 27, 52]. …”
Section: Discussionmentioning
confidence: 99%
“…In contrast, 87.5% of the animals showed a serum antibody response against Escherichia coli secreted protein B (EspB) at the same time that their faecal sample was positive for EHEC O157, O26 or, O111 or 6 weeks after a positive faecal sample. These antibodies persisted, even when shedding had ceased, until the animals were slaughtered, which was 2–8 months later, whereas EspA-specific antibodies disappeared within 2 months [9]. These results indicate that farm animals, which develop an immune response after infection, can become reinfected by different EHEC strains as evidenced by intermittent excretion which may reflect suppression of certain pathways of the immune system by the primary infection, making cattle more prone to persistent colonization by a subsequent infection.…”
Section: Introductionmentioning
confidence: 99%
“…Considering that EspB can be found in EPEC and EHEC, both related to severe diarrhea cases in human, its diagnosis and prevention are of great value for public health. Besides, since cattle are a natural reservoir of EHEC and a source for human infection, veterinary diagnosis and prevention are of major importance as well ( 37 , 38 ). Vaccine strategies to prevent EPEC and EHEC infections employing EspB as an antigen have been proposed in mice and cattle, contemplating the veterinary field ( 18 , 23 25 ).…”
Section: Discussionmentioning
confidence: 99%