Use of asynchrony in flowering for easy and economical polyhaploid induction in wheat following Imperata cylindrica‐mediated chromosome elimination approach

Abstract

Wheat × Imperata cylindrica‐mediated approach of doubled haploidy breeding requires hand emasculation followed by pollination with I. cylindrica pollen. The pace of this endeavour can be enhanced by utilizing asynchronous flowering of wheat spikes by direct pollination without emasculation followed by morphological marker–assisted screening of selfed and crossed seeds. The emasculated and un‐emasculated spikes of 13 spring and six winter wheat genotypes and two triticale × wheat derivatives were pollinated with I. cylindrica pollen. The response of different genotypes for production of crossed and selfed seeds with direct pollination varied significantly within and between groups for spring and winter wheats, whereas triticale × wheat derivatives responded similarly to each other but significantly different from spring and winter wheats. Although, the proportion of pseudoseed formation was lower in case of direct pollination, yet in some genotypes, it was comparable to that of pollination after emasculation. Moreover, the response for haploid embryo induction frequency was similar in both the cases. The method of direct pollination can be utilized for easy and economical induction of haploids.

Complex characterization of oat (Avena sativaL.) lines obtained by wide crossing with maize (Zea maysL.)

“…On the other hand, unlikely hybrids with genomes from both the parental species are not always obtained. Uniparental chromosome elimination, the removal of the one parent chromosomes from the wide hybrids, might be a benefit to the breeders for fast genetic improvement of the crop cultivars ( Chaudhary et al, 2013 ).…”

“…In an early stage of development of a hybrid embryo, chromosomes of the female are eliminated and developed haploid embryos contain only chromosomes of cultivated barley ( Liu et al, 2014 ). Chromosome elimination has been described in crosses of Triticum aestivum and Triticeae species ( H. vulgare and H. bulbosum ) as well as more distantly related species ( Zea mays , Pennisetum glaucum , Sorghum bicolor , Coix lacryma-jobi , Imperata cylindrica ) ( Barclay, 1975 ; Laurie & Bennett, 1986 ; Laurie & Bennett, 1988 ; Laurie, 1989 ; Inagaki & Mujeeb-Kazi, 1995 ; Mochida & Tsujimoto, 2001 ; Gernand et al, 2005 ; Komeda et al, 2007 ; Chaudhary et al, 2013 ).…”

New protocol for colchicine induced efficient doubled haploidy in haploid regenerants of tetraploid and hexaploid wheats at In vitro level

“…During the last few years, chromosome elimination approach has proved to be the efficient system of doubled haploidy breeding as compared to other biotechnological tools like androgenesis mediated approach (Chu et al 1973;Ouyang et al 1973;Wang et al 1973). Chromosome elimination-mediated doubled haploidy breeding includes the various approaches viz., bulbosum technique (Barclay 1975), wheat × maize system Bennett 1987, 1988) and the recently discovered wheat × Imperata cylindrica system which has emerged as the best choice for haploid induction in wheat (Chaudhary et al 2005(Chaudhary et al , 2013a(Chaudhary et al , 2013bChaudhary 2008Chaudhary , 2009Chaudhary , 2010aChaudhary , 2010bKaila et al 2012) as well as in durum wheat (Mahato and Chaudhary 2015). Despite immense importance and advantages of doubled haploidy (DH) breeding, its extensive adoption in crop improvement programmes is lacking because of low frequency of production of doubled haploids due to high mortality rate of regenerated haploid plants after colchicine treatment.…”

Peer Review #2 of "Complex characterization of oat (Avena sativa L.) lines obtained by wide crossing with maize (Zea mays L.) (v0.1)"

“…Manuscript to be reviewed haploid embryos contain only chromosomes of cultivated barley (Liu et al 2014). Chromosome elimination has been described in crosses of Triticum aestivum and Triticeae species (H. vulgare and H. bulbosum) as well as more distantly related species (Zea mays, Pennisetum glaucum, Sorghum bicolor, Coix lacryma-jobi, Imperata cylindrica) (Barclay 1975;Laurie andBennett 1986, 1988;Laurie 1989;Inagaki and Mujeeb-Kazi 1995;Mochida and Tsujimoto 2001;Gernand et al 2005;Komeda et al 2007;Chaudhary et al 2013).…”