Use of ATP Bioluminescence for Rapid Detection and Enumeration of Contaminants: The Milliflex Rapid Microbiology Detection and Enumeration System

Chollet, Renaud; Ribault, Sébastien

doi:10.5772/37055

Cited by 18 publications

(21 citation statements)

References 48 publications

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“…In the ATP bioluminescence method (ATP-B), the bioluminescence mechanism involves the enzyme luciferase in the presence of luciferin, oxygen (O 2 ), magnesium and ATP. This reaction leads to the emission of photons and the intensity of the light produced is directly proportional to the rate of ATP [ 117 ]. However, this method is not applicable to the genus Aspergillus , and to creams or suspensions, since these latest could interfere with the detection of light emission [ 116 ].…”

Section: Preservation Strategiesmentioning

confidence: 99%

Cosmetics Preservation: A Review on Present Strategies

et al. 2018

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Cosmetics, like any product containing water and organic/inorganic compounds, require preservation against microbial contamination to guarantee consumer’s safety and to increase their shelf-life. The microbiological safety has as main goal of consumer protection against potentially pathogenic microorganisms, together with the product’s preservation resulting from biological and physicochemical deterioration. This is ensured by chemical, physical, or physicochemical strategies. The most common strategy is based on the application of antimicrobial agents, either by using synthetic or natural compounds, or even multifunctional ingredients. Current validation of a preservation system follow the application of good manufacturing practices (GMPs), the control of the raw material, and the verification of the preservative effect by suitable methodologies, including the challenge test. Among the preservatives described in the positive lists of regulations, there are parabens, isothiasolinone, organic acids, formaldehyde releasers, triclosan, and chlorhexidine. These chemical agents have different mechanisms of antimicrobial action, depending on their chemical structure and functional group’s reactivity. Preservatives act on several cell targets; however, they might present toxic effects to the consumer. Indeed, their use at high concentrations is more effective from the preservation viewpoint being, however, toxic for the consumer, whereas at low concentrations microbial resistance can develop.

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Section: Preservation Strategiesmentioning

confidence: 99%

Cosmetics Preservation: A Review on Present Strategies

et al. 2018

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“…The best example is the ATP bioluminescence assay, which is based on the use of an ATP marker to detect presence of bacteria. The reaction assay is based on the D-luciferin oxidation by the luciferase, where the emitted light from the bioluminescent reaction is proportional to the ATP concentration [ 12 , 13 ]. This method is considered one of the most sensible and rapid (NRT) for microbial detection; its only downside is the need for expensive reagents.…”

Section: Introductionmentioning

confidence: 99%

Continuous and Real-Time Detection of Drinking-Water Pathogens with a Low-Cost Fluorescent Optofluidic Sensor

Simões

Dong

2018

Sensors

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Growing access to tap water and consequent expansion of water distribution systems has created numerous challenges to maintaining water quality between the treatment node and final consumer. Despite all efforts to develop sustainable monitoring systems, there is still a lack of low cost, continuous and real time devices that demonstrate potential for large-scale implementation in wide water distribution networks. The following work presents a study of a low-cost, optofluidic sensor, based on Trypthopan Intrinsic Fluorescence. The fluorospectrometry analysis performed (before sensor development) supports the existence of a measurable fluorescence output signal originating from the tryptophan contained within pathogenic bacteria. The sensor was mounted using a rapid prototyping technique (3D printing), and the integrated optical system was achieved with low-cost optical components. The sensor performance was evaluated with spiked laboratory samples containing E. coli and Legionella, in both continuous and non-continuous flow situations. Results have shown a linear relationship between the signal measured and pathogen concentration, with limits of detection at 1.4 × 103 CFU/mL. The time delay between contamination and detection of the bacteria was practically null. Therefore, this study supports the potential application of tryptophan for monitoring drinking water against water pathogens.

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“…Bioluminescent detection systems, used for in vivo and in vitro analytical methods, have been in the spotlight of research in the past decades (Jiang et al 2018;Xu et al 2016;Sadikot and Timothy 2005;Chollet and Ribault 2012;Sato et al 2004). The advantage of these systems over fluorescent systems lies in their superior sensitivity and easy handling (De Saint-Hubert et al 2012;Hu et al 2012).…”

Section: Introductionmentioning

confidence: 99%

Synthesis of N-Peptide-6-Amino-d-Luciferin Conjugates with Optimized Fragment Condensation Strategy

Kovács

Hegyes²,

Szebeni

et al. 2018

Int J Pept Res Ther

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The synthesis of peptide-luciferin conjugates has a pivotal role in the development of bioluminescent detection systems that are based on the determination of protease enzyme activity. This work describes the optimized synthesis of an N-peptide-6-amino-d-luciferin conjugate (Fmoc-Gly-Pro-6-amino-d-luciferin) with a simple fragment condensation method in adequate yields. Fmoc-Gly-Pro-6-amino-d-luciferin was produced from a previously synthesized Fmoc-Gly-Pro-OH and also previously synthesized 6-amino-2-cyanobenzothiazole with an optimized method, to which conjugate cysteine was added in an also improved way. The resulting conjugate was successfully used in a bioluminescent system, in vitro, demonstrating the applicability of the method. Graphical AbstractKeywords Bioluminescence · Protease activity · Aminoluciferin · Conjugate · Fragment condensation Electronic supplementary material The online version of this article (https ://doi.org/10.1007/s1098 9-018-9768-8) contains supplementary material, which is available to authorized users.

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Use of ATP Bioluminescence for Rapid Detection and Enumeration of Contaminants: The Milliflex Rapid Microbiology Detection and Enumeration System

Cited by 18 publications

References 48 publications

Cosmetics Preservation: A Review on Present Strategies

Cosmetics Preservation: A Review on Present Strategies

Continuous and Real-Time Detection of Drinking-Water Pathogens with a Low-Cost Fluorescent Optofluidic Sensor

Synthesis of N-Peptide-6-Amino-d-Luciferin Conjugates with Optimized Fragment Condensation Strategy

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