Use of ATP bioluminescence to determine the bacterial sensitivity threshold to a bacteriocin

Valat, Charlotte; Champiat, Dominique; Nguyen, Thuong Thi; Loiseau, Gérard; Raimbault, Maurice; Montēt, Didier

doi:10.1002/bio.735

Cited by 17 publications

(15 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To learn whether eDNA is associated with release of other cellular content, we measured the concentrations of ATP in the supernatants of S. sanguinis and S. gordonii wild-type strains and Pox Ϫ mutants grown under H 2 O 2 -producing conditions. ATP has been used as indicator of nisininduced cell lysis (45). The S. gordonii wild type and the Pox Ϫ mutant yielded similar amounts of extracellular ATP (eATP), maximizing at 180 min (Fig.…”

Section: Vol 191 2009 Dna Release By S Sanguinis and S Gordonii 6285mentioning

confidence: 99%

“…Consistent with this conclusion, DNA release appeared independent of release of other intracellular components, like ATP. ATP has been used to monitor nisin-induced lysis of lactobacilli (45). Interestingly, RNA was detected in the supernatant of S. gordonii and S. sanguinis during initial growth, but the concentration then declined over time.…”

Section: Vol 191 2009 Dna Release By S Sanguinis and S Gordonii 6285mentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Hydrogen Peroxide-Induced DNA Release by Streptococcus sanguinis and Streptococcus gordonii

et al. 2009

View full text Add to dashboard Cite

Extracellular DNA (eDNA) is produced by several bacterial species and appears to contribute to biofilm development and cell-cell adhesion. We present data showing that the oral commensals Streptococcus sanguinis and Streptococcus gordonii release DNA in a process induced by pyruvate oxidase-dependent production of hydrogen peroxide (H 2 O 2 ). Surprisingly, S. sanguinis and S. gordonii cell integrity appears unaffected by conditions that cause autolysis in other eDNA-producing bacteria. Exogenous H 2 O 2 causes release of DNA from S. sanguinis and S. gordonii but does not result in obvious lysis of cells. Under DNA-releasing conditions, cell walls appear functionally intact and ribosomes are retained over time. During DNA release, intracellular RNA and ATP are not coreleased. Hence, the release mechanism appears to be highly specific for DNA. Release of DNA without detectable autolysis is suggested to be an adaptation to the competitive oral biofilm environment, where autolysis could create open spaces for competitors to invade. Since eDNA promotes cell-to-cell adhesion, release appears to support oral biofilm formation and facilitates exchange of genetic material among competent strains.The release of bacterial DNA into the environment is of recent interest since this polymer is now recognized to stabilize cell-to-cell adherence and biofilm architecture (1,35,37). Treatment of extracellular DNA (eDNA) with DNase results in reduced intercellular stickiness, consistent with an adhesive function for eDNA. Furthermore, eDNA from Neisseria meningitis appears to have sufficient structural integrity to transform competent strains (11), indicating chromosomal origin. Since the abundance of eDNA is influenced by growth conditions, DNA release can also be regulated (40).DNA release is typically a consequence of cell lysis. Linked to DNA release, genetic transformation is the natural ability of competent bacterial species to take up DNA from the environment (13,34,42). During competence development, Streptococcus pneumoniae DNA is released by lysis of a subpopulation of cells (30,42). Cell lysis and DNA release are controlled in a cell density-dependent signal transduction process. The S. pneumoniae comX regulon, carrying late competence genes, also includes the murein hydrolase genes lytA and cbpD (19,42). Murein hydrolases digest structural components of the peptidoglycan, contributing to remodeling, recycling, and daughter cell separation. Furthermore, murein hydrolases trigger autolytic cell wall digestion, leading to release of DNA and other cellular content into the environment (36). The autolysis of bacterial cells as part of a regulated death program seems to be an important source for eDNA in diverse species, including Staphylococcus aureus (4, 36, 37), Staphylococcus epidermidis (35), Enterococcus faecalis (44), and Pseudomonas aeruginosa (1). In these species, the eDNA contributes to biofilm formation as a component of the extracellular biofilm matrix (35,37,44).Unlike for cell lysis-dependent release, the oral...

show abstract

Section: Vol 191 2009 Dna Release By S Sanguinis and S Gordonii 6285mentioning

confidence: 99%

Section: Vol 191 2009 Dna Release By S Sanguinis and S Gordonii 6285mentioning

confidence: 99%

Characterization of Hydrogen Peroxide-Induced DNA Release by Streptococcus sanguinis and Streptococcus gordonii

et al. 2009

View full text Add to dashboard Cite

show abstract

“…The effectiveness threshold, corresponding to a 100% inhibition of L. cremoris growth, was obtained for a null concentration of intracellular nucleotides. For an initial concentration of 1.4×10 7 bacteria/mL, the nisin effectiveness threshold was 3.4±0.01 mg nisin/L (Valat et al 2003). Compared to traditional assay of antibiotic residues in milk by acid production, ATP bioluminescence is rapid and less complex (Hawronskyj et al 1993).…”

Section: Detection Of Pesticide Residuesmentioning

confidence: 98%

Optical biosensors for food quality and safety assurance—a review

et al. 2011

View full text Add to dashboard Cite

Food quality and safety is a scientific discipline describing handling, preparation and storage of food in ways that prevent food borne illness. Food serves as a growth medium for microorganisms that can be pathogenic or cause food spoilage. Therefore, it is imperative to have stringent laws and standards for the preparation, packaging and transportation of food. The conventional methods for detection of food contamination based on culturing, colony counting, chromatography and immunoassay are tedious and time consuming while biosensors have overcome some of these disadvantages. There is growing interest in biosensors due to high specificity, convenience and quick response. Optical biosensors show greater potential for the detection of pathogens, pesticide and drug residues, hygiene monitoring, heavy metals and other toxic substances in the food to check whether it is safe for consumption or not. This review focuses on optical biosensors, the recent developments in the associated instrumentation with emphasis on fiber optic and surface plasmon resonance (SPR) based biosensors for detecting a range of analytes in food samples, the major advantages and challenges associated with optical biosensors. It also briefly covers the different methods employed for the immobilization of bio-molecules used in developing biosensors.

show abstract

“…Live and disrupted cells can be distinguished by the ratio of extra and intracellular ATP. For example, a method is described in [62] for quantitative determination of bacte ria sensitivity to nisin -a broad spectrum antibiotic affecting Gram positive bacteria that is widely used as a preservative. Under the action of nisin, cells release ATP, ADP, and AMP into the environment.…”

Section: Determination Of Intracellular Atp In a Mixed Culture Of Micmentioning

confidence: 99%

Bioluminescence assay for cell viability

Lomakina

Modestova

Ugarova

2015

Biochemistry Moscow

View full text Add to dashboard Cite

Theoretical aspects of the adenosine triphosphate bioluminescence assay based on the use of the firefly luciferin-luciferase system are considered, as well as its application for assessing cell viability in microbiology, sanitation, medicine, and ecology. Various approaches for the analysis of individual or mixed cultures of microorganisms are presented, and capabilities of the method for investigation of biological processes in live cells including necrosis, apoptosis, as well as for investigation of the dynamics of metabolism are described.

show abstract

Use of ATP bioluminescence to determine the bacterial sensitivity threshold to a bacteriocin

Cited by 17 publications

References 12 publications

Characterization of Hydrogen Peroxide-Induced DNA Release by Streptococcus sanguinis and Streptococcus gordonii

Characterization of Hydrogen Peroxide-Induced DNA Release by Streptococcus sanguinis and Streptococcus gordonii

Optical biosensors for food quality and safety assurance—a review

Bioluminescence assay for cell viability

Contact Info

Product

Resources

About