Use of Bacillus velezensis SDTB022 against tobacco black shank (TBS) and the biochemical mechanism involved

Qiu, Yue; Yan, Haohao; Sun, Shoumin; Wang, Yongqiang; Zhao, Xizeng; Wang, Hongyan

doi:10.1016/j.biocontrol.2021.104785

Cited by 12 publications

(13 citation statements)

References 42 publications

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“…It can grow under conditions of 7% NaCl and pH 5.7, producing acid and no gas. It can use various sugars to secrete urease and amylase, which is consistent with the experimental results of Qiu [ 30 ]. Based on the morphological characteristics, physiological and biochemical reaction characteristics of the colonies, and the identification manual of common bacterial systems and Berger’s bacteria, strain Z-1 was preliminarily identified as B. velezensis.…”

Section: Resultssupporting

confidence: 86%

Screening and Characteristics of Marine Bacillus velezensis Z-1 Protease and Its Application of Enzymatic Hydrolysis of Mussels to Prepare Antioxidant Active Substances

et al. 2022

Molecules

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Bacillus velezensis is a type of microorganism that is beneficial to humans and animals. In this work, a protease-producing B. velezensis strain Z-1 was screened from sludge in the sea area near Qingdao (deposit number CGMCC No. 25059). The response surface methodology was used to analyze protease production, and the optimal temperature was 37.09 °C and pH 7.73 with the addition of 0.42% NaCl, resulting in maximum protease production of 17.64 U/mL. The optimum reaction temperature and pH of the protease of strain Z-1 were 60 °C and 9.0, respectively. The protease had good temperature and pH stability, and good stability in solvents such as methanol, ethanol and Tween 80. Ammonium, NH4+,and Mn2+ significantly promoted enzyme activity, while Zn2+ significantly inhibited the enzyme activity. The protease produced by strain Z-1 was used for the enzymolysis of mussel meat. The mussel hydrolysate exhibited good antioxidant function, with a DPPH free radical removal rate of 75.3%, a hydroxyl free radical removal rate of 75.9%, and a superoxide anion removal rate of 84.4%. This study provides a reference for the application of B. velez protease and the diverse processing applications of mussel meat.

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Section: Resultssupporting

confidence: 86%

Screening and Characteristics of Marine Bacillus velezensis Z-1 Protease and Its Application of Enzymatic Hydrolysis of Mussels to Prepare Antioxidant Active Substances

et al. 2022

Molecules

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“…Bacillus spp., the most widely used biocontrol agents, are known for their effectiveness in controlling various plant diseases. Bacillus has an array of biocontrol mechanisms including secretion of hydrolytic enzymes and siderophores, production of secondary metabolites, and induction of systemic disease resistance in plants ( Zheng et al., 2021 ; Chu et al., 2022 ; Qiu et al., 2022 ; Wang et al., 2022 ; Zhang et al., 2022 ; Liu et al., 2023 ). Bacillus can also improve plant growth by producing IAA, solubilizing phosphorus, resisting abiotic stress, and fixing nitrogen, making it a common plant growth-promoting bacteria (PGPB) ( Sun et al., 2017 ; Wang et al., 2017 ; Jiao et al., 2020 ; Liu et al., 2023 ).…”

Section: Discussionmentioning

confidence: 99%

“…These enzymes play a role in the metabolism of disease-resistant secondary compounds (such as lignin, phenolic compounds, and plant defense hormones) ( Richter et al., 2012 ; Shine et al., 2016 ), the metabolism of active oxygen species ( Nakata et al., 2002 ; Singh et al., 2016 ), and directly inhibiting and killing pathogenic bacteria, thus enhancing the plants’ resistance to pathogens ( Wei et al., 1992 ). Several studies have demonstrated a positive correlation between the activity of POD, PPO, PAL, CAT, SOD, and other enzymes and the disease resistance index, which represents a specific protective reaction against pathogens ( Kashyap et al., 2021 ; Qiu et al., 2022 ). In this study, the application of B. velezensis B4-7 significantly enhanced the activity of various defense-related enzymes in tobacco plants, and this effect became more pronounced with increasing concentration.…”

Section: Discussionmentioning

confidence: 99%

Screening, identification and evaluation of an acidophilic strain of Bacillus velezensis B4-7 for the biocontrol of tobacco bacterial wilt

Meng,

Wang,

et al. 2024

Front. Plant Sci.

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Tobacco (Nicotiana tabacum L.) bacterial wilt, caused by Ralstonia solanacearum, is indeed a highly destructive plant disease, leading to substantial damage in tobacco production. While biological control is considered an effective measure for managing bacterial wilt, related research in this area has been relatively limited compared to other control methods. In order to discover new potential antagonistic bacteria with high biocontrol efficacy against tobacco bacterial wilt, we conducted an analysis of the microbial composition differences between disease-suppressive and disease-conducive soils using Illumina sequencing. As a result, we successfully isolated six strains from the disease-suppressive soil that exhibited antibacterial activity against Ralstonia solanacearum. Among these strains, B4-7 showed the strongest antibacterial activity, even at acidic conditions with a pH of 4.0. Based on genome analysis using Average Nucleotide Identity (ANI), B4-7 was identified as Bacillus velezensis. In greenhouse and field trials, strain B4-7 significantly reduced the disease index of tobacco bacterial wilt, with control efficiencies reaching 74.03% and 46.88% respectively. Additionally, B4-7 exhibited plant-promoting abilities that led to a 35.27% increase in tobacco production in field conditions. Quantitative real-time (qPCR) analysis demonstrated that strain B4-7 effectively reduced the abundance of R. solanacearum in the rhizosphere. Genome sequencing and Liquid Chromatography-Mass Spectrometry (LC-MS) analysis revealed that strain B4-7 potentially produces various lipopeptide metabolites, such as microlactin, bacillaene, difficidin, bacilysin, and surfactin. Furthermore, B4-7 influenced the structure of the rhizosphere soil microbial community, increasing bacterial abundance and fungal diversity, while also promoting the growth of different beneficial microorganisms. In addition, B4-7 enhanced tobacco’s resistance to R. solanacearum by increasing the activities of defense enzymes, including superoxide dismutase (SOD), phenylalanine ammonia-lyase (PAL), peroxidase (POD), catalase (CAT), and polyphenol oxidase (PPO). Collectively, these findings suggest that B. velezensis B4-7 holds significant biocontrol potential and can be considered a promising candidate strain for eco-friendly management of tobacco bacterial wilt.

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“…Microbial-induced systemic resistance is an important aspect of the biological control of plant diseases. Enzymes such as PPO, PAL, and POD are associated with systemic-induced resistance in plant tissues ( Qiu et al, 2022 ). In this study, B. siamensis YC-9 was demonstrated to have the ability to enhance the activity of POD, PAL, and PPO in cucumber roots when the seedlings were challenged with FOC, which contributed to a reduction in disease severity.…”

Section: Discussionmentioning

confidence: 99%

Termite Nest Associated Bacillus siamensis YC-9 Mediated Biocontrol of Fusarium oxysporum f. sp. cucumerinum

Zhou

Wang

et al. 2022

Front. Microbiol.

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The antagonistic potential of bacteria obtained from the nest of Odontotermes formosanus was assessed against Fusarium oxysporum f. sp. cucumerinum (FOC). Of 30, seven termite nest-associated bacteria strains had biocontrol potential. Among them, the strain YC-9 showed the strongest antifungal activity toward FOC. Phylogenetic analysis of the 16S rRNA amplified product of YC-9 revealed its identification as Bacillus siamensis. The in vivo antifungal activity experiment showed that the application of YC-9 at 108 cfu/ml significantly reduced the cucumber wilt incidence with a control efficacy of 73.2%. Furthermore, plant growth parameters such as fresh weight, dry weight, plant height, and root height were significantly improved by 42.6, 53.0, 20.8, and 19.3%, respectively. We found that inoculation with B. siamensis YC-9 significantly increased the activity of defensive enzymes such as peroxidase (POD), polyphenol oxidase (PPO), and phenylalanine ammonia-lyase (PAL) in diseased cucumber roots, thereby raising the resistance. PCR using gene-specific primers revealed that B. siamensis YC-9 contains biosynthetic genes for known antibiotics, including bacillomycin, iturin, and surfactin. Chemical analysis of the cultivation of B. siamensis YC-9 resulted in the isolation of five metabolites, including hexadecanoic acid (1), cyclo-(L-phenylalanylglycine) (2), cyclo-(L-trans-Hyp-L-Leu) (3), C15-surfactin (4), and macrolactin A (5), the structures of which were identified by the analysis of NMR spectroscopic data and MS. Among them, the compound 4 showed significant antifungal activity against conidial germination of FOC with an IC50 value of 5.1 μg/ml, which was comparable to that of the positive control, cycloheximide (IC50 value of 2.6 μg/ml). Based on these findings, this study suggests that termite-nest associated B. siamensis YC-9 could be a potential biological control agent for integrated control of soil-borne diseases like cucumber Fusarium wilt.

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Use of Bacillus velezensis SDTB022 against tobacco black shank (TBS) and the biochemical mechanism involved

Cited by 12 publications

References 42 publications

Screening and Characteristics of Marine Bacillus velezensis Z-1 Protease and Its Application of Enzymatic Hydrolysis of Mussels to Prepare Antioxidant Active Substances

Screening and Characteristics of Marine Bacillus velezensis Z-1 Protease and Its Application of Enzymatic Hydrolysis of Mussels to Prepare Antioxidant Active Substances

Screening, identification and evaluation of an acidophilic strain of Bacillus velezensis B4-7 for the biocontrol of tobacco bacterial wilt

Termite Nest Associated Bacillus siamensis YC-9 Mediated Biocontrol of Fusarium oxysporum f. sp. cucumerinum

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