Use of bacterial biosensors to interpret the toxicity and mixture toxicity of herbicides in freshwater

Strachan, Gillian; Preston, Sara; Maciel, H.; Porter, A.J.; Paton, Graeme I.

doi:10.1016/s0043-1354(01)00065-3

Cited by 41 publications

(20 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The cyanobacterial biosensor sensed the toxicity of herbicides at the EC 50 level for four (all except the triazine class) of the five groups tested and at the EC 20 level for all five of the herbicide groups tested. In contrast, the E. coli HB101 biosensor detected toxicity at the EC 50 level for only two herbicide classes (paraquat and triazine) of four tested (glyphosate not tested) and at the EC 20 level for three types (all except the urea type) of four tested (39). Furthermore, the cyanobacterial biosensor was more sensitive to the toxicity of herbicides than the E. coli HB101 biosensor; the EC 50 s and EC 20 s detected with the cyanobacterial biosensor were much lower than those with the E. coli HB101 biosensor.…”

Section: Discussionmentioning

confidence: 84%

“…When assay conditions (such as pH) were optimized, the cyanobacterial biosensor produced reproducible responses to a wide variety of herbicides (Table 2). Only one other study (39) has reported the effects of all the herbicides we tested except glyphosate on the bioluminescence response of lux-marked bacterial biosensors. The cyanobacterial biosensor was much more sensitive to the range of herbicides tested than the luxmarked E. coli HB101 biosensor (39).…”

Section: Discussionmentioning

confidence: 99%

“…Only one other study (39) has reported the effects of all the herbicides we tested except glyphosate on the bioluminescence response of lux-marked bacterial biosensors. The cyanobacterial biosensor was much more sensitive to the range of herbicides tested than the luxmarked E. coli HB101 biosensor (39). The cyanobacterial biosensor sensed the toxicity of herbicides at the EC 50 level for four (all except the triazine class) of the five groups tested and at the EC 20 level for all five of the herbicide groups tested.…”

Section: Discussionmentioning

confidence: 99%

“…Each assay was carried out in triplicate. The units of luminescence were then amended to RLU as before to allow comparison with the bioluminescence levels of lux-marked bacterial biosensor assays reported previously (39).…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Novel Cyanobacterial Biosensor for Detection of Herbicides

Shao

Howe

Porter

et al. 2002

Appl Environ Microbiol

View full text Add to dashboard Cite

The aim of this work was to generate a cyanobacterial biosensor that could be used to detect herbicides and other environmental pollutants. A representative freshwater cyanobacterium, Synechocystis sp. strain PCC6803, was chromosomally marked with the luciferase gene luc (from the firefly Photinus pyralis) to create a novel bioluminescent cyanobacterial strain. Successful expression of the luc gene during growth of Synechocystis sp. strain PCC6803 cultures was characterized by measuring optical density and bioluminescence. Bioluminescence was optimized with regard to uptake of the luciferase substrate, luciferin, and the physiology of the cyanobacterium. Bioassays demonstrated that a novel luminescent cyanobacterial biosensor has been developed which responded to a range of compounds including different herbicide types and other toxins. This biosensor is expected to provide new opportunities for the rapid screening of environmental samples or for the investigation of potential environmental damage.

show abstract

Section: Discussionmentioning

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Novel Cyanobacterial Biosensor for Detection of Herbicides

Shao

Howe

Porter

et al. 2002

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Agrochemicals may be transported from the treated soils in aquatic environment by waterborne runoff or by direct deposition from the atmosphere [3]. Microtox test is among the widely used bioassays for the toxicity determination of various compounds, including agrochemical substances [3][4][5][6]. The aim of this study was the evaluation of toxic response of two agrochemicals and copper, when act alone or in combination, using the Microtox test.…”

mentioning

confidence: 99%

Toxic and interactive toxic effects of agrochemical substances and copper on Vibrio fischeri

Κungolos¹,

Tsiridis²,

Samaras³

et al. 2006

WIT Transactions on Ecology and the Environment, Vol 99

View full text Add to dashboard Cite

The toxic and interactive toxic effects of two agrochemicals (fosthiazate and metalaxyl-M) and copper were investigated in this study on the photobacterium Vibrio fischeri. The toxicities of all tested compounds were generally comparable. The order of toxicity was: copper > fosthiazate > metalaxyl-M. The interactive effects of fosthiazate and metalaxyl-M mixtures were found to be additive for most of the concentration combinations tested. For the binary mixtures of fosthiazate and copper the interactive effect was antagonistic for all concentration combinations tested. Both agrochemicals showed a similar toxic response, while the toxicity of copper increased significantly by the increase of exposure time and a steep increase of copper toxicity was observed with a small increase of its concentration.

show abstract

Luminescent Bacterial Biosensors for the Rapid Detection of Toxicants

Davoren¹

2004

Water Encyclopedia

View full text Add to dashboard Cite

There is an increasing demand for sensitive and user‐friendly biological assays for the rapid and cost effective assessment of environmental contaminants in soil, sediment, and water ecosystems. Luminescent bacterial biosensors have emerged as promising tools in environmental monitoring for the sensitive and specific quantification of both toxicity and bioavailability. The naturally luminescent marine bacterium Vibrio fischeri has been extensively employed to assess the toxicity of a wide range of pollutants. In addition, recent advances in reporter gene technology have led to the development of many luminescent‐marked bacterial biosensors. Some of the bacterial biosensors that have been constructed are used for general toxicity testing, as they constitutively express luminescence and respond to toxicants in a “lights off” manner. Another class of biosensors have been developed which react to toxicants in a “lights on” manner, where light production is induced by bioavailable concentrations of toxicants. These microbial biosensors offer a powerful new approach to environmental monitoring, providing an indication of the bioavailabilty of specific pollutants in complex environments, rather than the total concentration obtained by traditional analytical techniques. Here the focus is on the use of both natural and genetically engineered luminescent bacterial biosensors for the assessment of toxicity and bioavailability.

show abstract

Use of bacterial biosensors to interpret the toxicity and mixture toxicity of herbicides in freshwater

Cited by 41 publications

References 17 publications

Novel Cyanobacterial Biosensor for Detection of Herbicides

Novel Cyanobacterial Biosensor for Detection of Herbicides

Toxic and interactive toxic effects of agrochemical substances and copper on Vibrio fischeri

Luminescent Bacterial Biosensors for the Rapid Detection of Toxicants

Contact Info

Product

Resources

About