Use of bacterial endospore with longer shelf-life in improved retting of jute

Chattopadhyay, Lipi; Majumdar, B.; Mazumdar, S.P.; Saha, Anupam; Saha, Ratul; Barai, Shrestha

doi:10.22438/jeb/40/2/mrn-854

Cited by 5 publications

(3 citation statements)

References 16 publications

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“…These three strains of Bacillus pumilus were later identified as Bacillus safensis, Bacillus velezensis and Bacillus altitudinis through whole genome sequencing based taxonomic affiliation of the isolates (Datta et al, 2020). The endospores of microbial consortium showed higher resistance to temperature, pH, UV irradiation and antibiotics than their vegetative forms and 18-month-old endospores can ret jute within 10 days with very good quality fibre (Chattopadhyay et al, 2019). Further, the immobilized strain of PJRB1 was found to have 132.6% higher xylanase production capacity over free cells after 72 hrs of incubation indicating scope of its industrial application for extra cellular xylanase production without any cellulase activity (Kundu and Majumdar, 2018).…”

Section: Introductionmentioning

confidence: 99%

An insight into the sequential changes in enzymatic activities during retting of jute (Corchorus spp. L.).

Majumdar¹,

Saha²,

Sarkar³

et al. 2021

JEB

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Aim: To study the dynamics of enzymes involved in biochemical process of jute (Corchorus spp.) retting with and without microbial retting consortium. Methodology: Two large scale retting trials were conducted with and without microbial retting consortium in triplicate. The retting water samples were collected every day at 24 hrs interval from both the trials. Polygalcturonase (PG), pectin lyase (PNL) and xylanase activities along with the pH were measured from the collected retting water samples following standard procedure. Fibre quality parameters were also studied from the resultant fibre obtained from both the retting trials. Results: There was a sharp decrease in pH of retting liquor by 1.35 units and that of pectin lyase activity by 97.9 Uml-1 within 24 hrs of inoculation of microbial retting consortium. Thereafter, higher pectin lyase (123.1 Uml-1), polygalacturonase (3.56 Iuml-1) and xylanase (0.818 IUml-1) activities were recorded during middle stage of retting. The enzyme activities were lower and non-significant at last stage of retting (11-14 days). The completion of retting without microbial consortium took longer time due to lower enzymatic activities as compared to microbial consortium mediated retting. Interpretation: The PG, PNL and xylanase enzymes released by the microbial consortium during retting of jute helped in faster biodegradation of pectin and xylan compared to control retting. Hence, the pre retting treatment of jute with microbial consortium is suggested for quick retting.

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Section: Introductionmentioning

confidence: 99%

An insight into the sequential changes in enzymatic activities during retting of jute (Corchorus spp. L.).

Majumdar¹,

Saha²,

Sarkar³

et al. 2021

JEB

Self Cite

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“…High colony forming unit and higher release of pectinolytic and xylanolytic enzymes during retting of jute by 18 months old endospores resulted in completion of jute retting in 10 days with better quality jute fibre (Fibre strength of 27.6 g/tex and fibre finess of 2.51 tex) as compared to the fibre (Fibre strength of 21.8 g/tex and fibre finess of 2.84 tex) obtained by using talc based formulation. Thus the endospores of Bacillus strains can be used in place of talc based microbial formulation for higher shelf life of the product, faster retting and better fibre quality of jute (Chattopadhyay et al 2019).…”

Section: Molecular Approachmentioning

confidence: 99%

Improved microbial retting and quality jute (Corchorus spp.) fibre production in India – A review

et al. 2022

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Jute (Corchorus spp. L.), the second most important fibre crop next to cotton, is a biodegradable and eco-friendly crop and one of the main cash crops of eastern India. India is the largest producer of raw jute in the world earning about `2200 crores per annum through export of diversified jute goods for which quality jute fibre is needed. The jute fibre quality depends directly on retting process. In India, more than 90% jute farmers do not have the access to free flowing water, so they carry out jute retting in stagnant water. The quality of jute fibre is deteriorated in stagnant water retting because of several factors like less rainfall, repeated retting, absence of efficient retting microbes, direct use of mud, soil etc. In this review paper we have discussed the views of various research workers about the efficient retting microbes, their utilization as microbial consortium for faster retting and quality jute fibre production even in stagnant water retting. Latest molecular approaches for identification of retting microbes including whole genome sequencing of retting microbes and their utilization as talc based formulation, spore based liquid formulation, use of immobilized strain of efficient retting microbes for faster environment friendly jute retting towards quality jute fibre production under farmers’ field conditions have also been discussed in detail.

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“…A microbial consortium consisting of three novel pectinolytic bacterial strains was isolated from jute retting water; this microbial consortium has very high levels of polygalacturonase (PG), pectin lyase (PNL) and xylanase activities without any cellulase activity and was found to be very useful for retting jute and mesta [ 10 ]. Retting with CRIJAF SONA has been demonstrated under farmers’ field conditions across the jute and mesta growing states of India and found to be very useful for quick retting with quality fibre production [ 11 , 12 ]. The genome sequencing of the constituent bacteria (three species of Bacillus ) revealed that the genome sizes of those bacterial strains are ∼ 3.8 Mb, with 3729 to 4002 protein-coding genes.…”

Section: Introductionmentioning

confidence: 99%

The sequential microbial breakdown of pectin is the principal incident during water retting of jute (Corchorus spp.) bast fibres

Datta,

Chattopadhyay,

Barai

et al. 2024

BMC Plant Biol

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The extraction of bast fibres such as jute from plant stems involves the removal of pectin, hemicellulose, and other noncellulosic materials through a complex microbial community. A consortium of pectinolytic bacterial strains has been developed and commercialized to reduce the retting time and enhance fibre quality. However, there are currently no studies on jute that describe the structural changes and sequential microbial colonization and pectin loss that occur during microbe-assisted water retting. This study investigated the stages of microbial colonization, microbial interactions, and sequential degradation of pectic substances from jute bark under controlled and conventional water retting. The primary occurrence during water retting of bast fibres is the bacterially induced sequential breakdown of pectin surrounding the fibre bundles. The study also revealed that the pectin content of the jute stem significantly decreases during the retting process. These findings provide a strong foundation for improving microbial strains for improved pectinolysis with immense industrial significance, leading to a sustainable jute-based “green” economy.

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Use of bacterial endospore with longer shelf-life in improved retting of jute

Abstract: Aim :Methodology : Results:Interpretation:

Cited by 5 publications

References 16 publications

An insight into the sequential changes in enzymatic activities during retting of jute (Corchorus spp. L.).

An insight into the sequential changes in enzymatic activities during retting of jute (Corchorus spp. L.).

Improved microbial retting and quality jute (Corchorus spp.) fibre production in India – A review

The sequential microbial breakdown of pectin is the principal incident during water retting of jute (Corchorus spp.) bast fibres

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