Use of Bioassays for Assessment of Water-Extractable Ecotoxic Potential of Soils

Maxam, Gudrun; Rila, Jean-Paul; Dott, Wolfgang; Eisentraeger, Adolf

doi:10.1006/eesa.1999.1855

Cited by 38 publications

(12 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…One such ecotoxicological test is Microtox ® , where the effect of soil water leachates on the test organism, the marine bacterium Vibrio fischeri is studied in acute toxicity tests. V. fischeri has been used to assess the toxicity of PAH and arsenic, both as single compounds [23][24][25], in soil [26,27] and following a soil remediation process [28][29][30].…”

Section: Introductionmentioning

confidence: 99%

Laboratory and pilot scale soil washing of PAH and arsenic from a wood preservation site: Changes in concentration and toxicity

Elgh-Dalgren

Arwidsson

Camdzija

et al. 2009

Journal of Hazardous Materials

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Laboratory and pilot scale soil washing of PAH and arsenic from a wood preservation site: Changes in concentration and toxicity

Elgh-Dalgren

Arwidsson

Camdzija

et al. 2009

Journal of Hazardous Materials

View full text Add to dashboard Cite

“…Unfortunately, previous assays that used test microorganisms were often based on exposure to soil extracts in water or organic solvent (12,20,32,42,43), thereby ignoring interactions of the test organisms with adsorptive soil pollutants. Furthermore, microbial toxicity testing in soil has also too often been based on irrelevant microorganisms (e.g., Microtox) or experimental conditions.…”

mentioning

confidence: 99%

Solid-Phase Contact Assay That Uses alux-MarkedNitrosomonas europaeaReporter Strain To Estimate Toxicity of Bioavailable Linear Alkylbenzene Sulfonate in Soil

Brandt¹,

Pedersen²,

Sørensen³

2002

Appl Environ Microbiol

View full text Add to dashboard Cite

Information about in situ toxicity of the bioavailable pools of adsorptive soil pollutants is a prerequisite for proper ecological risk assessment in contaminated soils. Such toxicity data may be obtained by assays allowing for direct exposure of introduced test microorganisms to the toxicants, as they appear in solid solution equilibria in the natural soil. We describe a novel sensitive solid-phase contact assay for in situ toxicity testing of soil pollutants based on a recombinant bioluminescent reporter strain of Nitrosomonas europaea. A slurry of the reporter strain and soil sample was shaken for 1 h, after which bioluminescence was measured either directly (soil slurry protocol) or in the supernatant obtained after centrifugation (soil extract protocol). The assay was validated for both protocols by using linear alkylbenzene sulfonate (LAS) as a toxic and adsorptive model compound in the soil samples. Interestingly, LAS showed the same toxicity to the reporter strain with either soil incubation (both protocols) or pure culture, suggesting that adsorbed LAS pools contributed to the observed toxicity. The solid-phase contact assay that used the reporter strain of lux-marked N. europaea was slightly more sensitive for the detection of LAS toxicity in soil than activity-based assays targeting indigenous nitrifiers and much more sensitive than assays targeting indigenous heterotrophic microbes. We conclude that the new solid-phase contact assay, which is based on direct interaction of the test microorganisms with bioavailable pools of the toxicants in soil, provides a most sensitive and relevant method for evaluating the in situ toxicity and assessing the risks of soil contaminants.

show abstract

“…Obviously, the sensitivity of the luminescent bacteria tests for soil toxicity assessment depends on the soil contaminants, their bioavailability, and their modes of toxic action. The test and its di!erent modi"cations seem to detect the toxicity caused by PAHs, as also observed in this study, nitroaromatic compounds (Maxam et al, 2000), and chlorophenols (Laine et al, 1997) in studies of soil contamination and remediation.…”

Section: Discussionmentioning

confidence: 74%

“…However, in the assessment of the eventual environmental risk of soil contamination with hazardous substances or evaluation of soil remediation processes a set of di!erent trophic level biotests should be used, and also other environmental concerns like genotoxicity (Belkin et al, 1994) should be evaluated. This has proven to be essential, because various organisms and hence toxicity tests have di!erent sensitivities to various contaminants (Maxam et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

Microbial Toxicity Tests and Chemical Analysis as Monitoring Parameters at Composting of Creosote-Contaminated Soil

Ahtiainen

Valo²,

Järvinen

et al. 2002

Ecotoxicology and Environmental Safety

View full text Add to dashboard Cite

Use of Bioassays for Assessment of Water-Extractable Ecotoxic Potential of Soils

Cited by 38 publications

References 3 publications

Laboratory and pilot scale soil washing of PAH and arsenic from a wood preservation site: Changes in concentration and toxicity

Laboratory and pilot scale soil washing of PAH and arsenic from a wood preservation site: Changes in concentration and toxicity

Solid-Phase Contact Assay That Uses alux-MarkedNitrosomonas europaeaReporter Strain To Estimate Toxicity of Bioavailable Linear Alkylbenzene Sulfonate in Soil

Microbial Toxicity Tests and Chemical Analysis as Monitoring Parameters at Composting of Creosote-Contaminated Soil

Contact Info

Product

Resources

About