Use of bioreactor systems in the propagation of forest trees

Vidal, Nieves; Sánchez, Conchi

doi:10.1002/elsc.201900041

Cited by 50 publications

(38 citation statements)

References 99 publications

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“…Temporary or Continuous Immersion Systems (TIS, CIS) were successfully used to proliferate eight ink-resistant C. sativa × C. crenata genotypes [ 145 , 146 ]. The proportion of rootable shoots (tall, vigorous and with an actively growing apex) suitable to undergo the rooting process was increased, and subsequent acclimation produced good plant survival [ 147 ]. Protocols developed for rooting of chestnut include in vitro and ex vitro approaches ( Table 3 ).…”

Section: Acclimation Of In Vitro Rooted Microshootsmentioning

confidence: 99%

“…In this context, micropropagation in photoautotrophic conditions in bioreactors can improve rooting and acclimation of recalcitrant tree species, as well as reduce propagation costs and agar-based residues [ 144 , 147 ]. The application of photoautotrophic conditions (high light intensity, CO 2 supplementation, and sugar-free medium) to the multiplication and rooting phase of chestnut has been reported for some genotypes [ 150 ].…”

Section: Future Prospects Of Biotechnological Approaches On Armentioning

confidence: 99%

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Recent Advances in Adventitious Root Formation in Chestnut

Vielba

Vidal

José

et al. 2020

Plants

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The genus Castanea includes several tree species that are relevant because of their geographical extension and their multipurpose character, that includes nut and timber production. However, commercial exploitation of the trees is hindered by several factors, particularly by their limited regeneration ability. Regardless of recent advances, there exists a serious limitation for the propagation of elite genotypes of chestnut due to decline of rooting ability as the tree ages. In the present review, we summarize the research developed in this genus during the last three decades concerning the formation of adventitious roots (ARs). Focusing on cuttings and in vitro microshoots, we gather the information available on several species, particularly C. sativa, C. dentata and the hybrid C.sativa × C. crenata, and analyze the influence of several factors on the achievements of the applied protocols, including genotype, auxin treatment, light regime and rooting media. We also pay attention to the acclimation phase, as well as compile the information available about biochemical and molecular related aspects. Furthermore, we considerate promising biotechnological approaches that might enable the improvement of the current protocols.

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Section: Acclimation Of In Vitro Rooted Microshootsmentioning

confidence: 99%

Section: Future Prospects Of Biotechnological Approaches On Armentioning

confidence: 99%

Recent Advances in Adventitious Root Formation in Chestnut

Vielba

Vidal

José

et al. 2020

Plants

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“…Pemanfaatan bioreaktor pada awalnya adalah untuk kultivasi mikroba. Kemudian dengan semakin berkembangnya teknik kultur sel tanaman, maka bioreaktor digunakan juga untuk produksi metabolit sekunder dari sel tanaman, yang kemudian berkembang untuk propagasi tanaman dalam skala besar (Vidal & Sánchez, 2019). Penggunaan bioreaktor memungkinkan otomatisasi dalam prosedur propagasi tanaman juga menghemat biaya produksi (Georgiev et al, 2014).…”

Section: Pendahuluanunclassified

“…Salah satu jenis bioreaktor yang banyak digunakan untuk tujuan propagasi tanaman yaitu bioreaktor dengan perendaman sesaat, atau Temporary Immersion Bioreactor (TIB) (Vidal & Sánchez, 2019). TIB terdiri dari tiga komponen utama, yaitu bejana beserta selang penghubung, mesin pewaktu (timer), dan pompa.…”

Section: Pendahuluanunclassified

Propagasi in vitro tanaman kurma (Phoenix dactylifera L.) pada bioreaktor dengan perendaman sesaat

Saptari¹,

Sinta²,

Riyadi³

et al. 2020

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The cultivation of date palm in Indonesia has increased since the last decade. However, the superior date palm seedlings are still limited and most of them are imported from other countries. The mass supply of superior date palm seedlings can be provided by in vitro propagation in the bioreactor. Therefore, the research was conducted to develop a protocol of date palm in vitro propagation by using Temporary Immersion Bioreactor (TIB). The in vitro propagation was carried out through somatic embryogenesis technique using meristematic tissues isolated from offshoots of date palm female clone cv. Zambli as explants. The explants were sterilized and then cultured to produce embryogenic calli and somatic embryos. Afterwards, somatic embryos germination and plantlets formation were conducted in TIB with treatments of immersion period: 3, 10, and 30 minutes every 6 hours, with 8 replications, The results showed that the optimal somatic embryo germination in TIB was with the immersion period of 30 min every 6 h, resulting in the most formation of shoots and fresh biomass weight increment up to nearly threefold in 6 weeks. Thereafter, plantlets formation in TIB with immersion period of 10 min and 30 min every 6 h exhibited similar performances in producing more plantlets with higher total fresh weight and better vigor than those of 3 min every 6 h. However, there were more rooted plantlets in the TIB with immersion period of 10 min every 6 h. Based on the results, an in vitro propagation protocol via somatic embryogenesis in TIB has been successfully developed for mass propagation of date palm cv. Zambli, which produced plantlets with good vigor and rooting.

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“…The bioreactor is a modified liquid culture system where the immersion of explant happens periodically. In culture systems with temporary tissue immersion, it is clear that the immersion time is very important and decisive [9] since this factor determines nutrient uptake, which affects the biomass enhancement.…”

Section: Introductionmentioning

confidence: 99%

Biomass Enhancement of Stevia rebaudiana Bertoni Shoot Culture in Temporary Immersion System (TIS) RITA® Bioreactor Optimized in Two Different Immersion Periods

et al. 2021

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Stevia plant contains steviol glycosides, which are estimated to be 300 times sweeter than sucrose. However, conventional (in vivo) propagation of Stevia rebaudiana in Indonesia was not effective due to poor results. Therefore, an alternative method to propagate stevia plants is needed. One of them is by using in vitro method. Multiplication with a large quantity of stevia biomass in a relatively short period can be conducted by using TIS RITA® (Recipient for Automated Temporary Immersion System). This study aimed to evaluate the effect of the immersion period of the medium on growth and the medium bioconversion into the production of shoot biomass. The bioreactors were set up with 15 min and 30 min immersions periods, scheduled every 6 h, and incubated for 21 d. The result indicated that the immersion period affected the biomass and growth rate (µ). Amount of 30 min immersion showed a greater percentage of shoot multiplication, higher biomass, percentage of leaf growth, growth rate, and productivity compared to 15 min immersion. The pattern of sucrose, mineral, and inorganic compounds consumption followed the growth of plant biomass for both systems. In conclusion, 30 min immersion gave a greater efficiency medium bioconversion to plant biomass compared with 15 min immersion.

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Use of bioreactor systems in the propagation of forest trees

Cited by 50 publications

References 99 publications

Recent Advances in Adventitious Root Formation in Chestnut

Recent Advances in Adventitious Root Formation in Chestnut

Propagasi in vitro tanaman kurma (Phoenix dactylifera L.) pada bioreaktor dengan perendaman sesaat

Biomass Enhancement of Stevia rebaudiana Bertoni Shoot Culture in Temporary Immersion System (TIS) RITA® Bioreactor Optimized in Two Different Immersion Periods

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