Use of biotyping to trace the origin of Staphylococcus aureus in foods

Devriese, Luc; M, Yde; Godard, C.; Isigidi, B.K.

doi:10.1016/0168-1605(85)90027-3

Cited by 27 publications

(16 citation statements)

References 7 publications

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“…The reasons for this difference are not known. The simplified biotyping system of Devriese [4] for typing S. aureus has been useful in tracing the origin of this organism in animal food and in food industry [6,9,10,12,19]. The raw chicken meat examined in the present study was primarily contaminated by S. aureus belonging to the poultry biotype, suggesting that the isolates originate probably from live chickens.…”

Section: Discussionmentioning

confidence: 79%

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Prevalence and Characterization of Staphylococcus aureus and Enterotoxigenic Staphylococcus aureus in Retail Raw Chicken Meat Throughout Japan

et al. 2005

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ABSTRACT. A total of 444 samples of raw chicken meat (thighs, breasts, wings, livers, gizzards, hearts and ovaries) that retailed at 145 different supermarkets in 47 prefectures in Japan were examined for contamination with Staphylococcus aureus in association with its enterotoxigenicity. S. aureus was isolated from 292 (65.8%) of the samples, and from 131 of the 145 supermarkets. There was no significant difference in the detection rate of S. aureus according to the type of meat examined. About 80% of 714 isolates belonged to the poultry (57.1%) and human biotypes (22.1%). Seventy-eight (21.7%) of 360 isolates were enterotoxigenic and isolated from 78 samples in 53 supermarkets in 31 prefectures. Staphylococcal enterotoxins (SEs) produced were SEB (50 isolates), SEA (14), SEC (8), SED (2), SEA+SEB (2), and SEA+SEC (2). Most of the enterotoxigenic isolates belonged to the human and poultry biotypes, coagulase type VII, VIII or IV, and were lysed by phages of group III. Identical SE types, biotypes, coagulase types and pulsed-field gel electrophoresis (PFGE) patterns were shown in isolates from different types of meat at the same supermarket and from samples taken from different supermarkets in the same prefectures or in isolates from samples obtained from several different prefectures. Among the 50 SEB-producing isolates, 27 yielded three similar PFGE patterns that differed by only a few fragments, suggesting that they were closely related genetically. The three patterns were found in isolates of samples that retailed at 17 supermarkets in 11 prefectures, indicating that they may be disseminated among raw chicken meat in Japan. KEY WORDS: biotype, coagulase type, pulsed-field gel electrophoresis, Staphylococcus aureus, staphylococcal enterotoxin.

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Section: Discussionmentioning

confidence: 79%

“…Biotyping: Biotyping was carried out according to the simplified system established by Devriese et al [4,6,10], which uses four tests: the production of staphylokinase and β-hemolysin, coagulation of bovine plasma within 6 hr, and the type of growth on crystal violet agar.…”

Section: Samplesmentioning

confidence: 99%

Prevalence and Characterization of Staphylococcus aureus and Enterotoxigenic Staphylococcus aureus in Retail Raw Chicken Meat Throughout Japan

et al. 2005

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“…The isolate C17 showed the human biovar, coagulase type III, phage pattern 29/52A/79/47/53/ 75+ (phage group I·III), no production of enterotoxins or TSST-1, an antimicrobial resistance pattern of PCG/ABPC/ EM/GM/KM, and β-lactamase production. In contrast, the isolate F14 showed the human biovar, coagulase type III, phage pattern 6/47/53/54/75/83A/85+ (phage group III), production of enterotoxin C and TSST-1, an antimicrobial resistance pattern of PCG/ABPC/CEZ, and β-lactamase [5,7,14]. Fig.…”

mentioning

confidence: 99%

“…In the present study, we attempted to trace the ecological origins of the MRSA isolates by using the simplified biotyping scheme of Devriese et al [5,7,14], which seems to be a reliable system. The two isolates were found to belong to the human biovar, which is characteristic of strains of human origin, suggesting that the two MRSA isolates are transmitted incidentally by workers handling raw chicken meat during the process.…”

mentioning

confidence: 99%

“…Biotyping of the MRSA isolates was carried out according to the simplified system established by Devriese et al [5,7,14], which uses four tests for the production of staphylokinase and β-hemolysin, the coagulation of bovine plasma within 6 hr, and the type of growth on crystal violet agar. Coagulase was typed with a coagulase typing kit (Denka Seiken, Co., Ltd., Tokyo) with neutralizing rabbit antisera specific to the eight coagulase types I to VIII.…”

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confidence: 99%

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Characterization of Methicillin-Resistant <i>Staphylococcus aureus</i> Isolated from Retail Raw Chicken Meat in Japan

Kitai

Shimizu

Kawano

et al. 2005

The Journal of Veterinary Medical Science

122

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ABSTRACT. Two isolates of mecA-positive methicillin-resistant Staphylococcus aureus (MRSA) from retail raw chicken meat were characterized by phenotypic and genotypic methods. One isolate showed the human biovar, coagulase type III, phage group I • III, the lack of production of enterotoxins and TSST-1, and resistance to PCG/ABPC/EM/GM/KM. The other isolate showed the human biovar, coagulase type III, phage group III, production of enterotoxin C and TSST-1, and resistance to PCG/ABPC/CEZ. The biotyping results indicate that the two isolates showed characteristics of human S. aureus. They also harbored SCCmec type IV, which has prevalently been found in community-acquired MRSA isolates. This paper is the first publication regarding MRSA isolates from raw chicken meat in Japan. KEY WORDS: MRSA, raw chicken meat, SCCmec type IV.

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Poultry-Like pA+ Biotype of Staphylococcus aureus CC346/084 Clone in Human Population

Piechowicz

Garbacz

2016

Curr Microbiol

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The aim of the study was (1) to analyse the prevalence of P-like pA+ biotype of S. aureus in material from healthy and diseased individuals, not employed at slaughterhouses or meat processing plants, and (2) to analyse the relatedness of these strains and their genetic variability. The study included 344 strains of Staphylococcus aureus isolated from hospitalized patients with staphylococcal infections and from healthy carriers. The biotypes of S. aureus were determined on the basis of fibrinolysin and β-haemolysin production, coagulation of bovine plasma, and type of growth on crystal violet agar. Additionally, the strains were tested for the synthesis of protein A in order to distinguish between P-like pA+ and poultry biotypes. Fibrinolysin gene (sak) and methicillin resistance (mecA) were detected by means of PCR. The clonal structure of studied strains was analysed using pulsed field gel electrophoresis and sequencing of spa gene. Finally, the strains were typed with a basic set of 23 bacteriophages. The strains belonging to P-like pA+ biotype corresponded to nearly 20 % of all the studied strains. In contrast to the human biotype, they formed one clonal complex, spa-CC346/084. The P-like pA+ biotype strains did not synthesize fibrinolysin, lacked the sak gene, and showed susceptibility to methicillin. In contrast to the human biotype strains, they belonged mostly to phage group II. The P-like pA+ biotype strains, previously described solely in meat products and meat industry workers, can be also present in hospitalized patients and extra-hospital carriers. These strains form a single, fibrinolysin-negative, clonal complex t084/CC346.

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Use of biotyping to trace the origin of Staphylococcus aureus in foods

Cited by 27 publications

References 7 publications

Prevalence and Characterization of Staphylococcus aureus and Enterotoxigenic Staphylococcus aureus in Retail Raw Chicken Meat Throughout Japan

Prevalence and Characterization of Staphylococcus aureus and Enterotoxigenic Staphylococcus aureus in Retail Raw Chicken Meat Throughout Japan

Characterization of Methicillin-Resistant <i>Staphylococcus aureus</i> Isolated from Retail Raw Chicken Meat in Japan

Poultry-Like pA+ Biotype of Staphylococcus aureus CC346/084 Clone in Human Population

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