Use of bone marrow mesenchymal (stromal) stem cells in experimental ischemic stroke in rats

Abstract

The effects of human mesenchymal stem cells on neurological functions and behavioral reactions of animals and on damaged brain tissue were studied on the model of focal cerebral ischemia in rats. Homing and differentiation of transplanted mesenchymal stem cells were also studied. Significant regression of neurological disorders after cell transplantation was noted, no appreciable shifts were detected by magnetic resonance tomography. Homing of transplanted cells was detected mainly in the zone of focal ischemia. Some cells died, others exhibited signs of differentiation into neurons and glia.

Comparison of the therapeutic effects of bone marrow mononuclear cells and microglia for permanent cerebral ischemia

“…As stem cells, BMSCs can differentiate into multiple lineages. Previous research has shown that transplantation of BMSCs significantly reduces the impairment of neurologic function after cerebral ischemia [5–8]. BM-MNCs comprise mainly mesenchymal cells and hematopoietic cells [14].…”

“…Although studies have demonstrated the high therapeutic value of BMSCs for ischemic stroke in animals [5–8], BMSCs are generally considered more difficult to culture and purify than bone marrow mononuclear cells (BM-MNCs). BM-MNCs comprise mesenchymal and hematopoietic stem cells.…”

Therapeutic Potentials of Mesenchymal Stem Cells Derived from Human Umbilical Cord

“…Instead of MSCs derived from bone marrow [49], Ding et al [41] transplanted approximately 1×10 6 clonally expanded hUC-MSCs into the cortex of middle cerebral artery occlusion (MCAO) rat models and observed significantly improved neurological function as well as considerably increased cortical neuronal activity. The transplanted hUC-MSCs migrated towards the ischemic boundary zone and differentiated into glial, neuronal, doublecortin + , CXCR4 + , and vascular endothelial cells [41].…”

Transplantation of Adipose-Derived Stem Cells in Stroke

“…The reported sources of mesenchymal stem cells include bone marrow (Skvortsova et al 2008), adipose tissue (Gutierrez-Fernandez et al 2013a), embryo (Liu et al 2009), placenta (Kranz et al 2010), dental pulp and periodontal ligament (Moshaverinia et al 2014;Vasandan et al 2014). Other sources, including palatine tonsil (Janjanin et al, 2008), dermis (Feisst et al 2014), and skeletomuscular system (Aydin et al 2014;Mason et al 2014), have also been reported.…”