Use of bovine FSH for superovulation and embryo production in beef heifers

Bellow, R.A.; Staigmiller, R. B.; Wilson, J. Frank; Phelps, D.A.; Darling, A.

doi:10.1016/0093-691x(91)90355-h

Cited by 46 publications

(23 citation statements)

References 7 publications

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“…Nonetheless, the observation that ovarian follicular development and ovulation rate were significantly enhanced in inhibin-immunized heifers is consistent with the hypothesis that immuno¬ neutralization of inhibin secreted into the peripheral circulation leads to an increase in pituitary FSH secre¬ tion which, in turn, enhances ovarian activity. Recent reports of increased ovulation rates in heifers treated with pure bovine FSH produced by recombinant DNA technology (Looney, Bondioli, Hill & Massey, 1988;Bellows, Staigmiller, Wilson et al 1991) con¬ firm the critical importance of FSH in this response. It would appear that in heifers, as in sheep (Henderson, Savage, Ellen et al 1988), a relatively small increment in plasma FSH is sufficient to initiate or sustain the maturation of more ovulable follicles, presumably by exposing additional developing follicles to a level of FSH above their required individual 'threshold' values (Ireland, 1987).…”

Section: Discussionmentioning

confidence: 96%

Effect of active immunization of heifers against inhibin on plasma FSH concentrations, ovarian follicular development and ovulation rate

Glencross¹,

Bleach²,

McLeod³

et al. 1992

Journal of Endocrinology

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To study the effects of immunoneutralization of endogenous inhibin on gonadotrophin secretion and ovarian function, prepubertal heifers (n = 6) were actively immunized against a synthetic peptide replica of the N-terminal sequence of bovine inhibin alpha subunit (bI alpha(1-29)Tyr30) coupled to ovalbumin. In contrast to ovalbumin-immunized controls (n = 6), bI alpha(1-29)Tyr30-immunized heifers had detectable inhibin antibody titres (% binding to 125I-labelled bovine inhibin at 1:2000 dilution of plasma) of 17 +/- 3% (S.E.M.) at puberty, rising to 31 +/- 5% by the end of the study period 7 months later. Neither age (immunized: 295 +/- 8 days; controls: 300 +/- 5 days) nor body weight (immunized: 254 +/- 13 kg; controls 251 +/- 9 kg) at onset of puberty differed between groups. Although the difference did not reach statistical significance, mean plasma FSH concentrations recorded in inhibin-immunized heifers remained 35-40% higher than in controls throughout the 12-week period leading up to puberty (P = 0.14) and during nine successive oestrous cycles studied after puberty (P = 0.10). Plasma LH concentrations did not differ between groups at any time during the study. Inhibin immunization had no effect on oestrous cycle length (immunized: 19.8 +/- 0.5 days; controls: 19.9 +/- 0.5 days).(ABSTRACT TRUNCATED AT 250 WORDS)

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Section: Discussionmentioning

confidence: 96%

Effect of active immunization of heifers against inhibin on plasma FSH concentrations, ovarian follicular development and ovulation rate

Glencross¹,

Bleach²,

McLeod³

et al. 1992

Journal of Endocrinology

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“…Wilson et al [16] tested several treatment regimens using rbFSH and reported that embryo production increased as the dose of rbFSH administered increased. Interestingly, Bellow et al [15] reported increased embryo production but similar superstimulatory responses in beef heifers treated with 24 mg rbFSH compared with heifers treated with 18 mg rbFSH. In the present experiment, the mean number of ovulatory follicles and CL for the three treatments (Folltropin, B50-LArbFSH, and A100-LArbFSH) considered able to induce a superstimulatory response ranged from 16.6 to 25.7 and 15.9 to 19.1, respectively (Table 1).…”

Section: Discussionmentioning

confidence: 99%

Use of a single injection of long-acting recombinant bovine FSH to superovulate Holstein heifers: A preliminary study

et al. 2014

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Our objective was to compare several experimental preparations of a single injection of long-acting recombinant bovine FSH (rbFSH; types A and B) to a porcine pituitary-derived FSH (Folltropin) to superovulate Holstein dairy heifers. Nonlactating, nonpregnant virgin Holstein heifers (n = 56) aged 12 to 15 months were randomly assigned to one of four superstimulatory treatments. Beginning at a random stage of the estrous cycle, all follicles greater than 5 mm were aspirated. Thirty-six hours later, heifers received an intravaginal P4 device and superstimulatory treatments were initiated. Treatments were (1) 300 mg of pituitary-derived FSH (Folltropin) administered in eight decreasing doses over a period of 3.5 days; (2) a single injection of 50 μg of A-rbFSH; (3) a single injection of 100 μg of A-rbFSH; and (4) a single injection of 50 μg of B-rbFSH. All heifers received 25 mg PGF2α at 48 and 72 hours after the insertion of P4 device. At 84 hours after insertion, P4 devices were removed, and ovulation was induced 24 hours later with hCG (2500 IU). Heifers were inseminated at 12 and 24 hours after hCG treatment. The number of ovulatory follicles was greatest for heifers treated with Folltropin and B50-rbFSH, least for heifers treated with A50-rbFSH, and was intermediate for heifers treated with A100-rbFSH (25.7 ± 3.2, 18.9 ± 3.2, 5.9 ± 0.9, and 16.6 ± 3.1, respectively; P < 0.001). The number of corpora lutea was greatest for heifers treated with Folltropin, B50-rbFSH, and A100-rbFSH, and least for heifers treated with A50-rbFSH (19.1 ± 2.4, 16.1 ± 3.0, 15.9 ± 2.9, and 2.6 ± 0.9, respectively; P < 0.001). The number of good-quality embryos differed among treatments and was greatest for heifers treated with B50-rbFSH, Folltropin, and A100-rbFSH and least for heifers treated with A50-rbFSH (7.6 ± 2.4, 6.5 ± 1.7, 4.3 ± 1.5, and 0.8 ± 0.5, respectively; P < 0.001). In conclusion, a single injection of a preparation of long-acting rbFSH (either 100 μg of A-rbFSH or 50 μg of B-rbFSH but not 50 μg of A-rbFSH) produced similar superovulatory responses resulting in the production of good-quality embryos when compared with a pituitary-derived FSH preparation administered twice daily for 4 days. More studies using different types of cattle and different doses of rbFSH are needed to confirm the findings reported in this preliminary study.

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“…Serum was collected and stored at −20°C until progesterone radioimmunoassay (RIA) was performed. Progesterone was analyzed in all serum samples by RIA (Coat-a-Count tubes, Diagnostic Products Corporation, Los Angeles) with a previously validated assay [39]. The inter- and intra-assay CV were 1.82% and 1.5%, respectively and assay sensitivity was 0.08 ng/mL.…”

Section: Methodsmentioning

confidence: 99%

Effects of Fertility on Gene Expression and Function of the Bovine Endometrium

Minten

Bilby²,

Bruno

et al. 2013

PLoS ONE

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Infertility and subfertility are important and pervasive reproductive problems in both domestic animals and humans. The majority of embryonic loss occurs during the first three weeks of pregnancy in cattle and women due, in part, to inadequate endometrial receptivity for support of embryo implantation. To identify heifers of contrasting fertility, serial rounds of artificial insemination (AI) were conducted in 201 synchronized crossbred beef heifers. The heifers were then fertility classified based on number of pregnancies detected on day 35 in four AI opportunities. Heifers, classified as having high fertility, subfertility or infertility, were selected for further study. The fertility-classified heifers were superovulated and flushed, and the recovered embryos were graded and then transferred to synchronized recipients. Quantity of embryos recovered per flush, embryo quality, and subsequent recipient pregnancy rates did not differ by fertility classification. Two in vivo-produced bovine embryos (stage 4 or 5, grade 1 or 2) were then transferred into each heifer on day 7 post-estrus. Pregnancy rates were greater in high fertility than lower fertility heifers when heifers were used as embryo recipients. The reproductive tracts of the classified heifers were obtained on day 14 of the estrous cycle. No obvious morphological differences in reproductive tract structures and histology of the uterus were observed in the heifers. Microarray analysis revealed differences in the endometrial transcriptome based on fertility classification. A genome-wide association study, based on SNP genotyping, detected 7 moderate associations with fertility across 6 different chromosomes. Collectively, these studies support the idea that innate differences in uterine function underlie fertility and early pregnancy loss in ruminants. Cattle with defined early pregnancy success or loss is useful to elucidate the complex biological and genetic mechanisms governing endometrial receptivity and uterine competency for pregnancy.

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Use of bovine FSH for superovulation and embryo production in beef heifers

Cited by 46 publications

References 7 publications

Effect of active immunization of heifers against inhibin on plasma FSH concentrations, ovarian follicular development and ovulation rate

Effect of active immunization of heifers against inhibin on plasma FSH concentrations, ovarian follicular development and ovulation rate

Use of a single injection of long-acting recombinant bovine FSH to superovulate Holstein heifers: A preliminary study

Effects of Fertility on Gene Expression and Function of the Bovine Endometrium

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