Use of cauda epididymis extract as immunocontraceptive

Rişvanlı, Ali; Aydın, Muhterem; Kaygusuzoğlu, Erdal; Bulut, Hakan; Apaydin, A M; Timurkan, Hüseyin

doi:10.1016/s0010-7824(02)00406-7

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…In a study performed by Risvanli et al. (), ASA rate was higher in cows which were mated three or more times compared to single‐time insemination which is in agreement with present study. In contrast to present study, ASA level was found lowest in mares mated three or more times and in maiden animals but highest in non‐mated mares Risvanli et al.…”

Section: Discussionsupporting

confidence: 93%

Antisperm antibodies in repeat‐breeding cows: Frequency, detection and validation of threshold levels employing sperm immobilization, sperm agglutination and immunoperoxidase assay

Srivastava

Shinde

Mehrotra

et al. 2016

Reprod Domestic Animals

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Antisperm antibodies have been found in repeat-breeding(RB) cows, and those causing agglutination and/or immobilization of sperm are considered to be closely related to unexplained infertility. However, a standard protocol for identifying antisperm antibodies (ASA) in cattle is not validated. Therefore, an investigation was undertaken to evaluate sperm immobilization (SIT), sperm agglutination (SAT) and immunoperoxidase (IPT)assays for detection of ASA in serum and their respective threshold levels for confirmation. Animals (heifers, normally breeding, repeat-breeding and pregnant animals) that were free from IBR, brucellosis and uterine infections (screened by clinical examination) were included in the study. Sperm agglutinating, sperm immobilizing and antisperm antibodies evaluated by respective assay were significantly higher (p < .05) in RB cows compared to other groups. The SIT assay was able to identify 61% of RB caused by ASA, more than those employing SAT and IPT. Furthermore, a dilution rate of 1:5 and 1:80 (confirms 59.0 and 57.0% RB+ve)were sufficient to diagnose ASA by SAT and IPT, respectively. Results indicate the presence of __12.6% clumped spermatozoa and __ 2.6%(cut-off value) peroxidase-positive spermatozoa at 1:5 and 1:80 dilutions diagnosed with SAT and IPT, respectively, may be considered as repeaters arising out of ASA. Furthermore, study also showed the presence of lower incidence of ASA positivity in other groups of animals (heifer

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Section: Discussionsupporting

confidence: 93%

Antisperm antibodies in repeat‐breeding cows: Frequency, detection and validation of threshold levels employing sperm immobilization, sperm agglutination and immunoperoxidase assay

Srivastava

Shinde

Mehrotra

et al. 2016

Reprod Domestic Animals

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“…The concentrations of obtained protein were determined according to the instructions of the Bradford Assay (SIGMA, Co., St. Louis, MO, USA). The ELISA was adapted from Risvanli et al [20]. Briefly, the ovarian homogenate was diluted in pH of 9.6 carbonate buffer to a concentration of 100 mg/ml.…”

Section: Histological Examinationmentioning

confidence: 99%

A study of ovarian autotransplantation without vascular a pedicle in rats

Rişvanlı

Timurkan

Akpolat

et al. 2006

J Assist Reprod Genet

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Purpose: The study aimed to determine effects of FSH applications on follicle survival, development and hormone output and antigenicity of rat ovarian tissue autografts placed at subcutaneous or subperitoneal sites.Methods: A total of sixteen female rats were used in the study. The animals were divided into three groups. Ovaries were dissected and then transplanted under the peritoneum in the first group animals (n = 5) or under the skin in the second group animals (n = 6). And the animals in the third group (n = 5) were sham operated. Following operations, intramuscular injection of 8 IU of rhFSH were made daily to the animals in first and second groups from the first day of operation through thirty days. Vaginal irrigation samples were prepared daily from the animals for 30 days. The concentrations of serum estradiol and antiovarian antibodies in the blood were determined using ELISA on the last day Results: Results showed that cyclic variations were noticed in the samples of vaginal irrigation by day 30 in the animals of first and second groups. However, no significant differences were seen between groups. The concentration of blood serum estradiol was higher in the animals of first group. Decrease in numbers of primary follicles were found in the animals of second group and lesser corpus luteum were found in the animals of control group on the histopathological examinations of transplanted ovaries. All rats in the first and second groups were defined as seropositive for antiovarian antibodies. When the OD values were compared between first and second groups, it was identified that the OD values of rats in the first group was higher than it was seen in the second group.Conclusion: The ovarian transplantation without vascular pedicle in rats is characterized by follicular hyperplasia endocrinologically functional. Being seropositive of all rats in first and second groups in terms of antiovarian antibodies is an indicator to these antibodies does not affect the functions of transplanted ovaries. It is believed that the highness of OD values in the group which is transplanted beneath the peritoneum is based on the highness of estradiol concentrations in these animals.

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“…ELISA procedure was carried out with some modifications in the pre-described protocol [16]. In short, 50 ml Metastin (Calbiochem, Germany) diluted with carbonate buffer (pH 9.6) at a concentration of 100 mg/ml was first added in each well of the 96-well ELISA microtitre plates.…”

Section: Identification Of Anti-kisspeptin Antibodiesmentioning

confidence: 99%

The effects of Kisspeptin antibodies on delayed estrus in rats

Rişvanlı

Apaydın

Bulut³

et al. 2009

Gynecological Endocrinology

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In recent years, numerous studies have been conducted on the effects of Kisspeptin/GPR54 system on sexual cycle, which proved that this system regulated gonadotropin release through GnRH. This study aims to determine the effects of hyperimmune serum containing antibodies produced against Kisspeptin on the sexual cycle and GnRH receptors in rat pituitaries. To this end, five Wistar female rats were passively immunised using a hyperimmune serum obtained from two Wistar female rats against Kisspeptin 10. Another five rats were selected as the control group. Anti-Kisspeptin antibodies of the hyperimmunised rats in the serum were identified by ELISA. The sexual cycles of the rats were followed by the measurements of vaginal irrigations and the estradiol concentrations in their blood samples. The number of follicles and corpora lutea in their ovaries was determined through histopathological tests, and the GnRH receptors in their pituitaries were identified by immunohistochemistry. Consequently, strong seropositivity was detected in all passively immunised rats and the hyperimmune serum. However, no difference was found between the groups with regard to the number of estrous cycles observed, the interval between estrous periods, estradiol concentrations, the number of follicles and corpora lutea and immunohistochemical results.

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Use of cauda epididymis extract as immunocontraceptive

Cited by 4 publications

References 13 publications

Antisperm antibodies in repeat‐breeding cows: Frequency, detection and validation of threshold levels employing sperm immobilization, sperm agglutination and immunoperoxidase assay

Antisperm antibodies in repeat‐breeding cows: Frequency, detection and validation of threshold levels employing sperm immobilization, sperm agglutination and immunoperoxidase assay

A study of ovarian autotransplantation without vascular a pedicle in rats

The effects of Kisspeptin antibodies on delayed estrus in rats

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