Use of cereals as basal medium for the formulation of alternative culture media for fungi

Abstract

The feasibility of developing alternative media to different culture media particularly potato dextrose agar was assessed using local cereal species as the basal media. Three cereal meal extracts -corn, sorghum and millet -were prepared, using them as substitute for the potato in potato dextrose agar. Potato dextrose agar (PDA) was the standard set up with which the performances of the formulated media were compared. Eight genera of fungi (Aspergillus niger, Fusarium moniliforme, Penicillium sp., Cercospora sp., Curvularia palescens, Botryodiplopodia sp., Rhizopus sp. and Rhodotorula rubra) were isolated and pure cultures of each species aseptically inoculated onto the three different formulated media including PDA and allowed to grow. Their growths were measured at 24, 48, 72, and 96 h after inoculation, using diameter of growth as an index. The set up was repeated thrice for each species on the three formulated media and the control (PDA). Growth of all the fungal species were observed to be about the same or sometimes better in the formulated media relative to those on the standard set up, except for Rhodotorula rubra. The radius of growth of F. moniliforme had an average of 15 + 0.58 mm on corn-dextrose agar relative to 12 mm on PDA at 96 h while Cercospora sp. measured 30 + 0.58 mm on millet-meal dextrose agar relative to 37 + 1.16 mm at 48 h. Botryodiplopodia sp. grew through the whole diameter of the plate (covering the total length of the radius of 45 mm) in both sorghum-meal and PDA at 96 h.

Exploration of Fungi Growth on Media Formulated from Agro-allied Wastes

“…Laleye (2002) reported a modification of Potato medium supplemented with cow dung, Soy milk and other growth factor for cultivation of fungi. Also, Adesemoye and Adedire (2005) studied the feasibility of developing alternative media to Potato Dextrose Agar (PDA) using local cereal species as a basal media. They observed that all the fungal species grew to some extent better on the formulated media in relation to the standard set up.…”

EvaluatingPseudomonas aeruginosaas plant growth-promoting rhizobacteria in West Africa

“…Sterile pipettes were used to draw approximately 1 ml of 10 76 dilution aseptically onto one sterile centrimide agar plate and a hockey stick was used to spread the inoculum on the plate. The hockey stick was sterilized by dipping it in 70% (v/v) alcohol and passing through the Bunsen burner (Adesemoye & Adedire 2005). This was incubated aerobically for 18 -24 h in the incubator at 308C.…”

Production of Blastospore of EntomopathogenicBeauveria bassianain a Submerged Batch Culture

“…Corn meal has been generally considered a good substrate for fungal growth, has a high starch content (67.6~69.4%), and also contains other nutrients (Lee et al, 2008). Corn meal supported the high growth rate of a variety of fungal strains, such as Aspergillus niger , Fusarium moniliforme , Penicillium sp., Cercospora sp., Curvularia palesscens , Botryodiplopodia sp., Rhizopus sp., and Rhodotorula rubra (Adesemoye and Adedire, 2005). The growth of the 8 fungal species were noted to be approximately the same or occasionally better in corn meal medium as compared to those grown on PDA, a widely used but expensive commercial fungal medium.…”