12Oligomycins are macrolide antibiotics, produced by Streptomyces spp., show biological activities 13 to several microorganisms like bacteria, fungi, nematodes and peronosporomycetes. 14 Conidiogenesis, germination of conidia and formation of appressoria are crucial for a successful 15 disease cycle and pathogenicity of the filamentous fungal phytopathogen. The goal of this 16 research was to evaluate the effects of two oligomycins, oligomycin B and oligomycin F along 17 with a commercial fungicide Nativo® 75WG on hyphal growth, conidiogenesis, conidia 18 germination, appressoria formation, and disease development of a worrisome wheat blast fungus, 19 Magnaporthe oryzae Triticum (MoT) pathotype. Both oligomycins suppressed the growth of 20 MoT mycelia depending on the dose. Between the two natural products, oligomycin F displayed 21 the maximum inhibition of MoT hyphal growth accompanied by oligomycin B with minimum 22 inhibitory concentrations (MICs) of 0.005 and 0.05 µg/disk, respectively. The application of the 2 23 compounds also completely halted the conidia formation in the MoT mycelia in agar medium. A 24 further bioassay showed that these compounds significantly inhibited MoT conidia germination 25 and induced lysis; if germinated, induced abnormal germ tube and suppressed appressoria 26 formation. Interestingly, the application of these macrolides significantly inhibited wheat blast 27 disease on detached leaves of wheat. This is a first report on the inhibition of mycelial growth, 28 process of conidia formation, germination of conidia, morphological changes in germinated 29 conidia, and suppression of blast disease of wheat by oligomycins from Streptomyces spp. A 30further study is needed to evaluate the mode of action and field trials of these natural compounds 31 to consider them as biopesticides for controlling this devastating wheat killer. 32 33 3 46 through G. Different isomers are highly selective for disrupting mitochondrial metabolism [3, 4, 47 8, 10]. Although the biological activities of oligomycins on fungi and peronosporomycetes have 48 been reported, nothing is known about the effect of these natural products on a notorious wheat 49 blast fungus Magnaporthe oryzae Triticum (MoT). The bioactivities of oligomycins to different 50 classes of fungal species indicates that their targets may involve varieties of cellular processes, 51 such as inhibition of mycelial growth of Cladosporium cucumerinum, Magnaporthe grisea, 52 Colletotrichum lagenarium, Botrytis cinerea, Cylindrocarpon destructans, Fusarium culmorum, 53 Erysiphe graminis and Phytophthora capsici [3, 11], lysis and motility inhibition of downy 54 mildew zoospores (Plasmopara viticola) of grapevine, and inhibition of motility of zoospores of 55 damping-off phytopathogen, Aphanomyces cochlioides [4]. 56 57 The wheat blast fungus MoT is one of the most damaging pathogens of wheat [12-15]. The 58 fungal three-celled, hyalin, pyriform conidium is bound to the host surface by an adhesive 59 secreted from the tip of the conidium [14, 16...