Use of Circulating and Cellular miRNAs Expression in Forensic Sciences

Dumache, Raluca; Rogobete, Alexandru Florin; Săndesc, Dorel; Bedreag, Ovidiu Horea; Ciocan, Veronica; Muresan, Camelia; Stan, Adrian Tudor; Sandesc, Mihai; Dinu, Anca; Popovici, Sonia Elena; Enache, Alexandra

doi:10.1515/jim-2017-0074

Cited by 3 publications

(1 citation statement)

References 69 publications

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“…However, the changes in the serum expression levels of miRNA-221 and miR-NA-33a indicated significant differences between the TRE and placebo groups so that the reduction (normalized to the baseline) was significantly lower in the TRE group than in the placebo group although the expression level of miRNA-155 also showed less change in TRE group than in the placebo group. However, this was statistically not significant [33]. miRNA-155 is an oncogene or a tumour suppressor in different types of cancer [34].…”

Section: Data Are Expressed As Mean ± Se Ast -Aspartate Aminotransfer...mentioning

confidence: 89%

The effect of trehalose administration on the serum expression levels of microRNAs associated with lipid metabolism and autophagy process in patients with myocardial infarction - the post-hoc analysis of the TREAT-IR trial.

et al. 2023

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IntroductionThis study aimed to evaluate the TRE-induced changes in the serum expression levels of miRNAs associated with lipids metabolism and autophagy process in MI patients to assess the potential protective effects of TRE in these patients.Material and methodsThis post hoc investigation was performed on serum samples obtained from a pilot randomized, double blind, placebo-controlled clinical trial that recruited 14 men (aged 18-80) who had MI and systemic inflammation. The patients were randomized in a 2:1 ratio to either TRE (15 g/week, intravenous (IV) ad-ministration) (N=10) or placebo groups (equal volume of saline 0.9 %) (N=4) for a period of 12 weeks. To measure the relative serum expression levels of miRNA-155, miRNA-221, and miRNA-33, the SYBR Green qPCR method was used.ResultsmiRNA-155 showed significantly higher serum expression levels in the TRE group (2.772±0.73; P=0.009) when compared to the placebo group. Also, significant reductions in miRNA-155 (0.171±0.03; P=0.016), miRNA-221 (0.116±0.07; P=0.013), and miRNA-33a (0.076±0.07; P=0.025) were observed in the placebo group at the end of the study. Nevertheless, the reduction (normalized to the baseline) of the serum expres-sion levels of miRNA-221 (FC:0.87±0.20 vs FC:0.18±0.08; P=0.009) and miRNA-33a (FC:0.73±0.22 vs FC:0.13±0.08; P=0.025) were significantly less in TRE group than in the placebo group.ConclusionsIV TRE administration did not reduce the expression of miRNA-221 and miRNA33a as much as placebo. Keeping a steady state of the serum expression levels of these miRNAs associated with lipoproteins metabolism and autophagy in the TRE group might have protective effects in patients with MI.

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Section: Data Are Expressed As Mean ± Se Ast -Aspartate Aminotransfer...mentioning

confidence: 89%

The effect of trehalose administration on the serum expression levels of microRNAs associated with lipid metabolism and autophagy process in patients with myocardial infarction - the post-hoc analysis of the TREAT-IR trial.

et al. 2023

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Human Trafficking and DNA Analysis

Saiz

Álvarez-Cubero

Lorente-Remon

et al. 2020

Forensic DNA Typing: Principles, Applications and Advancements

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miRNAs: Perspective towards the use for body fluid identification

Praihirunkit

2020

Siriraj Med J

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Identification of body fluids provides an important lead for crime investigation by which it can give a clue about the nature of the case and assist crime reconstruction. In the last decade, miRNAs have emerged as promising markers for body fluid identification due to their cell- /tissue-specificities. miRNAs are a class of small noncoding RNAs with ~ 22 nucleotides in length and their small sizes enable them to be resistant to degradation. The possibility to adopt miRNA markers for body fluid identification has been studied in various forensically relevant body fluids. This review aims to give a comprehensive summary of proposed miRNA markers for identifying five body fluids (venous blood, menstrual blood, semen, vaginal secretion and saliva). Based on numerous evaluations of miRNA markers and the development of model analysis using a single panel of miRNAs to identify unknown samples, proposed panels and analysis strategies were gathered and discussed.

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Use of Circulating and Cellular miRNAs Expression in Forensic Sciences

Cited by 3 publications

References 69 publications

The effect of trehalose administration on the serum expression levels of microRNAs associated with lipid metabolism and autophagy process in patients with myocardial infarction - the post-hoc analysis of the TREAT-IR trial.

The effect of trehalose administration on the serum expression levels of microRNAs associated with lipid metabolism and autophagy process in patients with myocardial infarction - the post-hoc analysis of the TREAT-IR trial.

Human Trafficking and DNA Analysis

miRNAs: Perspective towards the use for body fluid identification

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