Cognitive enhancers (CEs) encompass a wide range of drugs, including prescription medications for attention deficit disorders and pharmacological compounds for cognitive enhancement. It is well-documented that the students are the leading cohort of CEs users. Exploring how healthcare professionals perceive the use of CEs for academic accomplishments is significant to understand their encouragement of CE use. Hence, the purpose of the current study was to look at healthcare professionals' attitudes and perceived understanding about the usage of CEs in academic contexts. The study was a quantitative cross-sectional research design conducted in different healthcare and academic settings of Karachi. The respondents were approached either through social media platforms or the official email addresses of their working organizations. Data were collected through a web link of an online questionnaire that included four sections; inquiring about the respondents' demographics characteristics, their knowledge about CEs, their attitudes towards the use and impact of CEs, and their inclination to use a hypothetical prescription-only CE. The response rate of the study was 73.3%. The majority of the respondents negated to permit university students to using CEs for cognitive boost (n ¼ 360, 67.1%), to concentrate (n ¼ 406, 75.7%), to increase vigilance (n ¼ 394, 73.5%) or to mitigate the effects of other medicines (n ¼ 312, 58.2%). The pharmacists were more likely to refute that using CEs by the students is safe (pharmacists 10.8% vs. physicians 8.3%, p¼<0.001), beneficial (pharmacists 12.7% vs. physicians 5.3%, p¼<0.001), or necessary (pharmacists 17.6% vs. physicians 12.8%, p¼<0.001). The major reasons for not encouraging the use of CEs were fear of misuse (n ¼ 510, 95.1%), safety concerns (n ¼ 495, 92.3%), and their consideration for CE as unnecessary medical intervention (n ¼ 441, 82.2%). The findings indicated that overall, respondents have a clear consensus of not letting university students use CEs for cognitive improvement or any other purpose implying that cognitive enhancement is not yet a common or approved medical practice by the healthcare professionals in Pakistan.