Use of Comet-FISH in the study of DNA damage and repair: Review

Glei, Michael; Hovhannisyan, Galina; Pool‐Zobel, Beatrice L.

doi:10.1016/j.mrrev.2008.01.006

Cited by 58 publications

(25 citation statements)

References 79 publications

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“…A combination of the comet assay with the fluorescent in situ hybridization, the Comet-FISH (Glei et al 2009;Glei and Schlörmann 2014), has been used to study DNA damage (Glei et al 2007) and DNA repair (Shaposhnikov et al 2011) in selected genes or particular DNA sequences. This assay allows monitoring the DNA damage repair of a specific gene by following the migration of gene-specific signals from the comet tail into the comet head in the course of time, as shown for the human tumor suppressor gene p53 in peripheral blood mononucleated cells (PBMCs) (Horvathova et al 2004).…”

Section: Dna Repairmentioning

confidence: 99%

Comet assay: an essential tool in toxicological research

2016

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The comet assay is a versatile, reliable, cost-efficient, and fast technique for detecting DNA damage and repair in any tissue. It is useable in almost any cell type and applicable to both eukaryotic and prokaryotic organisms. Instead of highlighting one of the numerous specific aspects of the comet assay, the present review aims at giving an overview about the evolution of this widely applicable method from the first description by Ostling and Johanson to the OECD Guideline 489 for the in vivo mammalian comet assay. In addition, methodical aspects and the influence of critical steps of the assay as well as the evaluation of results and improvements of the method are reviewed. Methodical aspects regarding oxidative DNA damage and repair are also addressed. An overview about the most recent works and relevant cutting-edge reviews based on the comet assay with special regard to, e.g., clinical applications, nanoparticles or environmental risk assessment concludes this review. Taken together, the presented overview raises expectations to further decades of successful applications and enhancements of this excellent method.

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Section: Dna Repairmentioning

confidence: 99%

Comet assay: an essential tool in toxicological research

2016

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“…36), which can be detected using the comet assay 37 or by monitoring activation of DNA damage response (DDR) proteins, such as ATR, ATM, Chk1, Chk2 or H2AX-γ (reviewed in refs. 38 and 39).…”

Section: Tumor Suppressors In Brafmentioning

confidence: 99%

Pathways of oncogene-induced senescence in human melanocytic cells

Bansal¹,

Nikiforov²

2010

Cell Cycle

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“…Unrejoined double-strand breaks are likely to be lethal, and misrejoined double-strand breaks can cause chromosome aberrations and cell death [3,14,15]. Unwinding of the DNA and electrophoresis at neutral pH (7,8) predominantly facilitates the detection of double-strand breaks and cross-links; unwinding and electrophoresis at pH 12.1-12.4 facilitates the detection of single-and double-strand breaks, incomplete excision repair sites and cross-links [16].…”

Section: Methodsmentioning

confidence: 99%

“…Another modifi cation of this method is the so-called Comet-FISH which is a useful tool for detection of region-specifi c DNA damage. Comet-FISH combines two well-established methods, the Comet assay with fl uorescence in situ hybridization (FISH) [7]. It is employed in diff erent kinds of probes to clarify the connection in the etiology of diff erent cancers to DNA susceptibility [8].…”

Section: Introduction To Comet Assaymentioning

confidence: 99%

“…It is employed in diff erent kinds of probes to clarify the connection in the etiology of diff erent cancers to DNA susceptibility [8]. Some working group use FISH for detection of chromosomal loci associated with cancer [7,9] and it is also possible to study the kinetics of a specifi c gene repair involved in cancer [10]. Th e comet assay has been applied in a broad range of scientifi c fi elds, including genetic toxicology, ecotoxicology and DNA repair research [3].…”

Section: Introduction To Comet Assaymentioning

confidence: 99%

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Application of the comet assay method in clinical studies

Fikrová

Štětina

Hronek

et al. 2011

Wien Klin Wochenschr

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The comet assay or single-cell gel electrophoresis (SCGE) assay is now widely accepted as a standard method for assessing DNA damage in individual cells. It finds use in a broad variety of applications including human biomonitoring, genotoxicology, ecological monitoring and as a tool for investigation of DNA damage and repair in different cell types in response to a range of DNA-damaging agents. The comet assay should be eminently suitable for use in clinical practice since it is a relatively simple and inexpensive technique which requires only a few cells, and results can be obtained within a matter of hours. This method can be used in the study of cancer as well as in lifestyle and dietary studies. In cancer it is useful for measuring DNA damage before, throughout and after therapy (either radiotherapy or chemotherapy). Another use of this method is in lifestyle study, such as investigation of the effect on DNA of common human activities (e.g. smoking, or working with a potentially genotoxic agent). The final use of comet assay in this paper is dietary study. In this type of study we observe the effects of consumption of specific foods or supplements which may be protective for DNA against damage.

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Use of Comet-FISH in the study of DNA damage and repair: Review

Cited by 58 publications

References 79 publications

Comet assay: an essential tool in toxicological research

Comet assay: an essential tool in toxicological research

Pathways of oncogene-induced senescence in human melanocytic cells

Application of the comet assay method in clinical studies

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