Use of Crayfish in Biomonitoring Studies of Environmental Pollution of the River Meuse

Schilderman, P.A.E.L.; Moonen, E.J.C.; Maas, L.M.; Welle, I.; Kleinjans, Jos

doi:10.1006/eesa.1999.1827

Cited by 75 publications

(46 citation statements)

References 37 publications

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“…The Meuse is the second largest river in The Netherlands. It is controlled by weirs and confined by dikes and groynes to guarantee year-round navigation (Admiraal et al, 1993;Schilderman et al, 1999;Pedroli et al, 2002). The load of heavy metals (pollution by mining) decreased since 1970, but inorganic nitrogen pollution increased over the last few decades (Vanderpoorten, 1999).…”

Section: Introductionmentioning

confidence: 98%

Regional differences in nutrient limitation in floodplains of selected European rivers: implications for rehabilitation of characteristic floodplain vegetation

et al. 2006

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Extremely high river discharges in 1993 and 1995 along the Dutch rivers Rhine and Meuse have increased the public awareness of possible safety threats. As a result the 'Space for Rivers' program was implemented, aiming at restoring physical space for the rivers in combination with ecological rehabilitation. However, the development of species-rich vegetation types in these floodplain areas is lagging behind restoration targets and biogeochemical constraints may play a vital role in this.Biogeochemical, hydrological and vegetation data were collected in 111 plots in both rehabilitated and original floodplains in regulated and more pristine river systems in The Netherlands and Poland. Soil nutrient and soil pore water data were summarized by factor analysis and the subsequent principal components were compared to vegetation and hydrological data by correlation analysis. The correlation analyses between vegetation parameters including nutrient stoichiometry and the biogeochemical soil variables resulted in a remarkable difference between pristine and impacted river systems. The results suggest a clear N-limitation of plant growth in pristine floodplains, and apparent absence of limitation in regulated, impacted floodplains. In addition, results indicate that flooding events do not lead to one-way transport of sediment and nutrients from the river into the floodplains; rather they indicate that highly dynamic hydrological conditions prevent soils from accumulating organic matter and nutrients.This study shows that nutrient limitation in regulated floodplains shifted from distinctly N-limited plant growth to no nutrient limitation at all, probably due to decades of high fertilizer and manure application and nutrient input by the rivers during flooding. The consequence of our findings for rehabilitation activities is that it might be necessary to restore nitrogen limitation in floodplain systems in order to create opportunities for a species-rich floodplain vegetation, through nutrient removal by haymaking.

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Section: Introductionmentioning

confidence: 98%

Regional differences in nutrient limitation in floodplains of selected European rivers: implications for rehabilitation of characteristic floodplain vegetation

et al. 2006

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“…Briefly, the assay involves DNA purification, digestion to normal and adduct-modified 3'-mononucleotides, removal of normal nucleotides (via enzymatic digestion, solvent extraction or chromatographic methods), 32 P-postlabelling at the 5' position of adducted nucleotides followed by chromatographic separation, detection and quantification (via autoradiography, scintillation counting or phosphor screen imaging analysis) [182]. Following this assay, PAHs cause the appearance of the diagonal radioactive zone (DRZ) (Figure 10) [173,178,183,184]. MS/MS analysis has recently emerged as a powerful tool in the detection and structure elucidation of DNA adducts as well as for their quantification at very low concentrations (as often present in biological samples).…”

Section: Dna Adducts From Polycyclic Aromatic Hydrocarbonsmentioning

confidence: 99%

Polycyclic Aromatic Hydrocarbons a Constituent of Petroleum: Presence and Influence in the Aquatic Environment

Pampanin¹,

Sydnes²

2013

Hydrocarbon

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“…They constantly have to accommodate the terms of the habitat. Crustaceans, due to the wide range of occurrence, sensitivity to xenobiotics (mainly heavy metals and organic pollutants), ability to accumulate and availability throughout the year, can be used as biomarkers in environmental monitoring (Schilderman et al 1999). The results of measurement of glutathione concentration very clearly show the seasonal variations.…”

Section: Gssgmentioning

confidence: 99%

The Use of Different MS Techniques to Determine Glutathione Levels in Marine Tissues

2012

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Several techniques were used, mainly mass spectrometry connected with gas chromatography, matrixassisted laser desorption with ionization time-of-flight and mass spectrometry connected with liquid chromatography. A major problem was encountered in determining glutathione, which can be conditioned by the pH and selected reducing agents. The GSH form can be oxidized through derivatization, and a small glutathione amount in the biological samples may hinder the determination process. Another problem is the existence of a metal ion in the tested organism; therefore, often a reagent with a chelating function is added to the sample and the mobile phase in liquid chromatography is applied with appropriate polarity for GSH and GSSG. We determined the concentrations of total, reduced, and oxidized glutathione in the liver, hepatopancreas, muscle, and gonad tissues of brown shrimp (Crangon crangon) and fish (Psetta maxima and Clupea harengus membras). The highest concentrations of tGSH were recorded in the shrimp hepatopancreas (7.21 ± 0.011 μmol g −1 wet weight), in herring liver (2.85±0.025 μmol g −1 ), and in turbot liver (1.86±0.063 μmol g −1). In turn, the highest concentrations were reported for GSSG in the muscle of shrimp (0.140±0.000204 μmol g ). We also investigated seasonal changes in the concentrations of glutathione in the muscle of C. crangon shrimp in the annual cycle. The lowest values of total glutathione were recorded during spring and autumn, which could be correlated with the increase in lipid peroxidation and oxidative stress.

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Use of Crayfish in Biomonitoring Studies of Environmental Pollution of the River Meuse

Cited by 75 publications

References 37 publications

Regional differences in nutrient limitation in floodplains of selected European rivers: implications for rehabilitation of characteristic floodplain vegetation

Regional differences in nutrient limitation in floodplains of selected European rivers: implications for rehabilitation of characteristic floodplain vegetation

Polycyclic Aromatic Hydrocarbons a Constituent of Petroleum: Presence and Influence in the Aquatic Environment

The Use of Different MS Techniques to Determine Glutathione Levels in Marine Tissues

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