Use of cryotubes for the cryopreservation of tambaqui fish semen (Colossoma macropomum)

Maria, Alexandre Nízio; Carvalho, Andre B. de; Araújo, Rafael Venâncio de; Santos, Jadson Pinheiro; Carneiro, Paulo César Falanghe; Azevedo, H. C.

doi:10.1016/j.cryobiol.2015.02.004

Cited by 23 publications

(29 citation statements)

References 23 publications

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“…Ovulation and spermiation of curimatã-pacu (Prochilodus argenteus) was induced with CPE (Arantes et al, 2011) and out-of-season sperm production was induced in pirapitinga (Piaractus brachypomus) after a single intramuscular injection (Nascimento et al, 2010). Finally in the tambaqui (Colossoma macropomum), intramuscular injections of 2 mg kg -1 of CPE increased sperm volume and decreased viscosity (Maria et al, 2015), as a routine method for Characiformes (Viveiros & Godinho, 2009). Also, various studies have been carried out to induce spermiation and improve the sperm cryopreservation techniques in several species of the genus Brycon (Characidae family) that comprises more than 70 fish species distributed in Central and South America.…”

Section: Gth Preparations Of Piscine Originmentioning

confidence: 99%

“…Abramis brama bream CPE 18-24 (Kucharczyk et al, 1997) Acipenser brevirostrum shortnose sturgeon CPE 24 (Horváth et al, 2005) Acipenser ruthenus sterlet CPE 24, 48, 72 /12-42 (Alavi et al, 2012;Dzyuba et al, 2012;Psenicka et al, 2008) Anguila japonica Japanese eel salmon PE 42 days 3 (Kagawa et al, 2009) Brycon amazonicus yamú CPE 24 (Velasco-Santamaría et al, 2006) Brycon insignis tiete tetra/piabanha CPE 8 (Viveiros et al, 2012b) Brycon opalinus pirapitinga-do-sul CPE 8 (Orfão et al, 2011) Brycon orbignyanus piracanjuba CPE 5 (Maria et al, 2006) Clarias gariepinus African catfish CPE, Clarias PE 24 (Viveiros et al, 2002) Colossoma macropomum tambaqui CPE 10 (Maria et al, 2015) Cyprinus carpio common carp CPE 24 (Li et al, 2013) Huso huso beluga CPE 48 (Linhartova et al, 2013) Odontesthes bonariensis pejerrey CPE, salmon PE 24 (Miranda et al, 2005) Piaractus brachypomus cirapitinga CPE 12 (Nascimento et al, 2010) Prochilodus argenteus curimatã-pacu CPE - (Arantes et al, 2011) Prochilodus lineatus curimba CPE 8 (Viveiros et al, , 2010 Silurus glanis European catfish CPE 24-48 (Linhart et al, 2004) Tinca tinca common tench CPE 24 (Linhart et al, 1995) 1 Ground pituitaries or pituitary extracts, mainly Carp Pituitary Extract (CPE), or pituitary extracts (PE) from other fishes.…”

Section: Speciesmentioning

confidence: 99%

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Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality

2017

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This article reviews the use of hormonal treatments to enhance sperm production in aquaculture fish and the methods available for evaluating sperm quality. The different types of testis development are examined and a brief review is presented of the endocrine regulation of spermatogenesis in fishes, including the increasing evidence of the existence of spermatozoa subpopulations. Hormonal manipulations are employed to induce spermatogenesis in species such as the freshwater eels, to synchronize maximal sperm volume to ovulation for in vitro fertilization and to enhance sperm production in species with poor spermiation. The hormones that are employed include gonadotropins (GtH) of piscine or mammalian origin, and gonadotropin-releasing hormone agonists (GnRHa) administered by injections or controlled-release delivery systems, with or without dopaminergic inhibitors. Pheromones in the culture water and hormones added to the sperm in vitro have also been employed to enhance spermiation and sperm quality, respectively, in some fishes. Hormonal therapies usually do not affect sperm quality parameters, except in cases where fish fail to spermiate naturally or produce very small volumes of high-density sperm. Different parameters have been used to evaluate fish sperm quality, including sperm volume and density, spermatozoa motility and morphometry, and seminal plasma composition. The development of Computer-Assisted Sperm Analysis (CASA) systems made possible the estimation of a higher number of sperm motion parameters using an objective, sensitive and accurate technique. The development of Assisted Sperm Morphology Analysis (ASMA) software has introduced a new approach for sperm evaluation studies, demonstrating changes in the spermatozoa related to reproductive season, hormonal treatments or the cryopreservation processes, and how these may be related to changes in sperm motility and fertilization capacity. The article concludes with a few practical protocols for the enhancement of sperm production in aquaculture species.

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Section: Gth Preparations Of Piscine Originmentioning

confidence: 99%

Section: Speciesmentioning

confidence: 99%

Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality

2017

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“…Semen analysis using CASA has been reported in other species of fish and has been shown to be a good tool to help determine sperm quality; it provides more information than subjective analysis and more accurate results (NYNCA et al, 2014;VIVEIROS et al, 2014;JUDYCKA et al, 2015;MARIA et al, 2015). This is one of the first studies using CASA in the analysis of fresh and cryopreserved sperm for P. brevis.…”

Section: Sperm Cryopreservation and Thawingmentioning

confidence: 99%

Cryopreservation of Prochilodus brevis semen: freezing media and thawing rates

Nunes

Oliveira

Lopes

et al. 2016

SCA

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Prochilodus brevis is a migratory fish, an important component of its river ecosystem and an appreciated animal in northeastern cuisine. However, human activities have threatened its survival. Thus, researchers have become interested in developing genetic material storage protocols, such as seminal cryopreservation. Therefore, determination of the appropriate freezing media and thawing rate is a fundamental step toward the use of this biotechnology in the production of common curimatã and for reducing risks to the species' survival. As such, the aim of the current study was to evaluate the effect of different freezing media and thawing rates on the quality of cryopreserved semen from P. brevis. For this study, males received a single dose of pituitary extract of carp 18 hours before semen collection. The semen samples were diluted in 5% glucose + 10% methyl glycol (MG), 5% glucose + 10% dimethyl sulfoxide (DMSO), 0.9% NaCl + 10% methyl glycol, and 0.9% NaCl + 10% dimethyl sulfoxide, loaded into 0.25-mL straws and frozen in liquid nitrogen vapor. The semen from each treatment was thawed at three different thawing rates: 25 °C for 30 sec, 30 °C for 16 sec and 40 °C for 12 sec. Motility, vitality and morphology analyses were performed by computer-assisted sperm analysis (CASA). The characteristics of the fresh sperm mostly resembled those found in the literature. For the parameters analyzed, fresh sperm presented higher sperm quality in comparison to all treatments with cryopreserved sperm (p<0.05), except for the characteristic of normal morphology, for which the sperm cryopreserved in glucose and MG did not differ statistically from the fresh sperm. For the cryopreserved semen, the greatest results of total motility and curvilinear velocity (VCL) were obtained using glucose and DMSO, regardless of the thawing rate employed. For the straight-line velocity (VSL) and average path velocity (VAP), DMSO showed the best results, regardless of the diluent and thawing rate. With regard to vitality, the highest values were achieved when DMSO and thawing rates of 30 °C for 16 sec or 40 °C for 12 sec were used. In the morphological analysis, the greatest percentage of normal sperm cells was obtained using thawing rates of 25 °C for 30 sec and 40 °C for 12 sec, regardless of the freezing media. Sperm quality was found to suffer interference from the freezing media, as well as from interaction between its components (diluent and cryoprotectant) and the thawing rate used. Under the methodological conditions employed, the use of 5% glucose + 10% DMSO and a thawing rate of 30 °C for 16 seconds or 40 °C for 12 seconds is recommended for P. brevis semen cryopreservation. ResumoO Prochilodus brevis é um peixe reofílico, importante componente do ecossistema fluvial e apreciado na culinária nordestina. No entanto, ações antrópicas têm ameaçado sua sobrevivência. Desta forma, surge, nos pesquisadores, o interesse no desenvolvimento de protocolos de conservação do material genético, como a criopreservação seminal. Logo, a deter...

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“…1) but there is an increasing interest in designing cryopreservation protocols for species from tropical and subtropical areas (Maria et al, 2015; Viveiros and Godinho, 2009). Traditional empirical studies in a factorial experimental design testing a range of freezing rates, cryoprotectants or extenders are still conducted in order to develop cryopreservation protocols for species such as Atlantic salmon (Dziewulska et al, 2011), beluga sturgeon (Aramli et al, 2015), Siberian sturgeon (Judycka et al, 2015), perch (Bernáth et al, 2015) or tambaqui (Maria et al, 2015; Varela Junior et al, 2015). Nevertheless, the evaluation of sperm quality after freezing/thawing has evolved, allowing a better understanding of the mechanisms linking to the compromised quality during the cryopreservation process.…”

Section: Fish Sperm Cryopreservationmentioning

confidence: 99%

Cryobanking of aquatic species

et al. 2017

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This review is focused on the applications of genome cryobanking of aquatic species including freshwater and marine fish, as well as invertebrates. It also reviews the latest advances in cryobanking of model species, widely used by the scientific community worldwide, because of their applications in several fields. The state of the art of cryopreservation of different cellular types (sperm, oocytes, embryos, somatic cells and primordial germ cells or early spermatogonia) is discussed focusing on the advantages and disadvantages of each procedure according to different applications. A special review on the need of standardization of protocols has also been carried out. In summary, this comprehensive review provides information on the practical details of applications of genome cryobanking in a range of aquatic species worldwide, including the cryobanks established in Europe, USA, Brazil, Australia and New Zealand, the species and type of cells that constitute these banks and the utilization of the samples preserved. Statement of relevance This review compiles the last advances on germplasm cryobanking of freshwater and marine fish species and invertebrates, with high value for commercial aquaculture or conservation. It is reviewed the most promising cryopreservation protocols for different cell types, embryos and larvae that could be applied in programs for genetic improvement, broodstock management or conservation of stocks to guarantee culture production.

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Use of cryotubes for the cryopreservation of tambaqui fish semen (Colossoma macropomum)

Cited by 23 publications

References 23 publications

Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality

Hormonal manipulations for the enhancement of sperm production in cultured fish and evaluation of sperm quality

Cryopreservation of Prochilodus brevis semen: freezing media and thawing rates

Cryobanking of aquatic species

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