Use of cyclic voltammetry to determine the antioxidant capacity of berry fruits: correlation with spectrophotometric assays

Nikolic, Milena; Pavlović, Aleksandra; Mitić, Snežana S.; Tošić, Snežana B.; Kaličanin, Biljana; Manojlović, Dragan; Stanković, Dalibor M.

doi:10.17660/ejhs.2019/84.3.5

Cited by 17 publications

(17 citation statements)

References 38 publications

(35 reference statements)

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“…Carajurin presented the first oxidation process at 0.47 V vs. Ag/Ag + (0.42 V vs. NHE) ( Figure 7 , green line), which can be attributed to the oxidation of the hydroxy substituent; the second oxidation peak is probably due to the formation of an intermediate radical produced during the oxidation process. Both oxidation processes showed some reversibility; however, the cathodic peaks showed slower processes, resulting in lower peak currents [ 17 ]. As shown in Figure 7 (green line), when scanning at more negative potentials, the carajurin in oxygen absence showed a reversible process in potential,1.07 V vs. Ag/Ag + (–1.12 V vs. NHE), which indicated the compound’s ability to capture electrons forming a radical anion, a factor that influences in the compound’s properties [ 18 , 19 ].…”

Section: Resultsmentioning

confidence: 99%

Carajurin Induces Apoptosis in Leishmania amazonensis Promastigotes through Reactive Oxygen Species Production and Mitochondrial Dysfunction

et al. 2022

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Carajurin is the main constituent of Arrabidaea chica species with reported anti-Leishmania activity. However, its mechanism of action has not been described. This study investigated the mechanisms of action of carajurin against promastigote forms of Leishmania amazonensis. Carajurin was effective against promastigotes with IC50 of 7.96 ± 1.23 μg.mL−1 (26.4 µM), and the cytotoxic concentration for peritoneal macrophages was 258.2 ± 1.20 μg.mL−1 (856.9 µM) after 24 h of treatment. Ultrastructural evaluation highlighted pronounced swelling of the kinetoplast with loss of electron-density in L. amazonensis promastigotes induced by carajurin treatment. It was observed that carajurin leads to a decrease in the mitochondrial membrane potential (p = 0.0286), an increase in reactive oxygen species production (p = 0.0286), and cell death by late apoptosis (p = 0.0095) in parasites. Pretreatment with the antioxidant NAC prevented ROS production and significantly reduced carajurin-induced cell death. The electrochemical and density functional theory (DFT) data contributed to support the molecular mechanism of action of carajurin associated with the ROS generation, for which it is possible to observe a correlation between the LUMO energy and the electroactivity of carajurin in the presence of molecular oxygen. All these results suggest that carajurin targets the mitochondria in L. amazonensis. In addition, when assessed for its drug-likeness, carajurin follows Lipinski’’s rule of five, and the Ghose, Veber, Egan, and Muegge criteria.

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Section: Resultsmentioning

confidence: 99%

Carajurin Induces Apoptosis in Leishmania amazonensis Promastigotes through Reactive Oxygen Species Production and Mitochondrial Dysfunction

et al. 2022

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“…This technique is commonly employed to investigate the reduction and oxidaqtion processes of molecular species, to which good correlations between the oxidation potentials of various antioxidant and their antioxidant efficiency have been found [ 37 ]. Indeed, strong correlations have been found between DPPH, ABTS, FRAP and reducing power assays and CV [ 38 ]. However, correlations of CV to metal chelation ability and advanced glycation end products formation are weak, implying that different mechanisms are behind the antioxidant effects [ 39 ].…”

Section: Resultsmentioning

confidence: 99%

Phenolic Profile and Bioactive Potential of Stems and Seed Kernels of Sweet Cherry Fruit

et al. 2020

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Every year, large quantities of stems and pits are generated during sweet cherry processing, without any substantial use. Although stems are widely recognized by traditional medicine, detailed and feasible information about their bioactive composition or biological value is still scarce, as well as the characterization of kernels. Therefore, we conducted a study in which bioactivity potential of extracts from stems and kernels of four sweet cherry cultivars (Early Bigi (grown under net cover (C) and without net cover (NC)), Burlat, Lapins, and Van) were examined. The assays included antioxidant (by 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic) acid (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH) and β-carotene-linoleic acid bleaching assays), and antibacterial activities against important Gram negative and Gram positive bacterial human isolates. Profile and individual phenolic composition of each extract were determined by High-performance liquid chromatography (HPLC) analysis. Extracts from stems of cv. Lapins and kernels of Early Bigi NC presented high levels of total phenolics, flavonoids, ortho-diphenols and saponins. Excepting for cv. Early Bigi NC, major phenolic compounds identified in stems and kernels were sakuranetin and catechin, respectively. In cv. Early Bigi NC the most abundant compounds were ellagic acid for stems and protocatechuic acid for kernels. In all extracts, antioxidant activities showed a positive correlation with the increments in phenolic compounds. Antimicrobial activity assays showed that only stem’s extracts were capable of inhibiting the growth of Gram positive isolates. This new data is intended to provide new possibilities of valorization of these by-products and their valuable properties.

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“…Quantitative data about the overall polyphenolic content can be estimated from the current intensities of the voltammetric scans [23,24] while individual concentrations of targeted polyphenols can be determined in combination with separation techniques such as liquid chromatography [24,25,26]. The comparison of voltammetric measurements with spectroscopic indexes has been investigated by several authors, for example, in the assessment of the antioxidant capacity of oenological tannins [27], berry fruits [28], propolis extracts [29], red wines [30], green tea [31] and hops [32]. In addition, electronic voltammetric tongues consisting of an array of modified graphite-epoxy composites plus a gold microelectrode have been used to predict the FC index of wines [33].…”

Section: Introductionmentioning

confidence: 99%

Exploring the Antioxidant Features of Polyphenols by Spectroscopic and Electrochemical Methods

2019

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This paper evaluates the antioxidant ability of polyphenols as a function of their chemical structures. Several common food indexes including Folin-Ciocalteau (FC), ferric reducing antioxidant power (FRAP) and trolox equivalent antioxidant capacity (TEAC) assays were applied to selected polyphenols that differ in the number and position of hydroxyl groups. Voltammetric assays with screen-printed carbon electrodes were also recorded in the range of −0.2 to 0.9 V (vs. Ag/AgCl reference electrode) to investigate the oxidation behavior of these substances. Poor correlations among assays were obtained, meaning that the behavior of each compound varies in response to the different methods. However, we undertook a comprehensive study based on principal component analysis that evidenced clear patterns relating the structures of several compounds and their antioxidant activities.

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Use of cyclic voltammetry to determine the antioxidant capacity of berry fruits: correlation with spectrophotometric assays

Cited by 17 publications

References 38 publications

Carajurin Induces Apoptosis in Leishmania amazonensis Promastigotes through Reactive Oxygen Species Production and Mitochondrial Dysfunction

Carajurin Induces Apoptosis in Leishmania amazonensis Promastigotes through Reactive Oxygen Species Production and Mitochondrial Dysfunction

Phenolic Profile and Bioactive Potential of Stems and Seed Kernels of Sweet Cherry Fruit

Exploring the Antioxidant Features of Polyphenols by Spectroscopic and Electrochemical Methods

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