Use of dermograph for improvement of PpIX precursor’s delivery in photodynamic therapy: Experimental and clinical pilot studies

Requena, Michelle Barreto; Russignoli, Pedro; Vollet‐Filho, José Dirceu; Sálvio, Ana Gabriela; Fortunato, Thereza Cury; Pratavieira, Sebastião; Bagnato, Vanderlei Salvador

doi:10.1016/j.pdpdt.2019.101599

Cited by 11 publications

(5 citation statements)

References 25 publications

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“…A more homogeneous PpIX distribution and a greater penetration in the tissue were observed for animal model using dermographs compared to the topical application of the cream only [46]. A pilot clinical trial was also performed in nodular BCC lesions using a dermograph for intradermal delivery in PDT, and no recurrence was observed after 28 months of follow-up [46].…”

Section: Dermographmentioning

confidence: 94%

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Strategies to Improve Drug Delivery in Topical PDT

Requena¹,

Stringasci²,

Vollet‐Filho³

et al. 2021

Photodynamic Therapy - From Basic Science to Clinical Research

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Topical photodynamic therapy (PDT) has been applied to treat premalignant and malignant lesions such as actinic keratosis and non-melanoma skin cancer. A limiting factor of the technique is cream permeation and studies using chemical and physical approaches to overcome it have increased over the years. This chapter is going to explore the main techniques described in the literature used to improve the cream permeation or the photosensitizer (PS) distribution concerning homogeneity. Outcomes-based on animal studies and clinical trials comparing different delivery techniques are going to be presented, highlighting the aspects of invasiveness, costs, harmfulness, and effectiveness of those methods.

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Section: Dermographmentioning

confidence: 94%

“…Usually, both frequency and depth can be adjusted in those devices, according to the application. The holes performed into the skin allow for greater skin penetration of liquids by capillarity [45,46].…”

Section: Dermographmentioning

confidence: 99%

Strategies to Improve Drug Delivery in Topical PDT

Requena¹,

Stringasci²,

Vollet‐Filho³

et al. 2021

Photodynamic Therapy - From Basic Science to Clinical Research

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“…Dermatography (medical tattooing) is used in surgical reconstruction and sometimes to improve the appearance of scars [ 151 ]. The use of dermatography to enhance MAL penetration was studied in animal models and in a Gorlin syndrome patient in whom six nBCC were treated with MAL (mixed with 0.015% EDTA) delivered by dermatography, resulting in clearance with no recurrence at 28 months [ 152 ].…”

Section: Potential Improvements In Topical Pdtmentioning

confidence: 99%

Photodynamic Therapy for Basal Cell Carcinoma: The Clinical Context for Future Research Priorities

Collier

Rhodes

2020

Molecules

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Photodynamic therapy (PDT) is an established treatment option for low-risk basal cell carcinoma (BCC). BCC is the most common human cancer and also a convenient cancer in which to study PDT treatment. This review clarifies challenges to researchers evident from the clinical use of PDT in BCC treatment. It outlines the context of PDT and how PDT treatments for BCC have been developed hitherto. The sections examine the development of systemic and subsequently topical photosensitizers, light delivery regimens, and the use of PDT in different patient populations and subtypes of BCC. The outcomes of topical PDT are discussed in comparison with alternative treatments, and topical PDT applications in combination and adjuvant therapy are considered. The intention is to summarize the clinical relevance and expose areas of research need in the BCC context, ultimately to facilitate improvements in PDT treatment.

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“…In terms of physical approaches as pretreatment, intradermal delivery has been evaluated by the association of microneedles (MNs) rollers [20][21][22][23], high-pressure needle-free injections [24][25][26], iontophoresis [27][28][29][30], laser [31,32], and, ultrasound [33,34]. Requena et al performed experimental and clinical studies using a micropigmentation machine (dermograph) and confirmed that the precursor and consequent formation of PpIX in-depth were more efficient with the intradermal delivery compared to the cream applied topically [35]. Mu et al applied successfully plum-blossom needles associated with pulsed CO 2 laser to enhance the PDT effect in patients with BCC [36].…”

mentioning

confidence: 99%

Dissolving microneedles containing aminolevulinic acid improves protoporphyrin IX distribution

Requena

Permana

Vollet‐Filho

et al. 2020

Journal of Biophotonics

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One important limitation of topical photodynamic therapy (PDT) is the limited tissue penetration of precursors. Microneedles (MNs) are minimally invasive devices used to promote intradermal drug delivery. Dissolving MNs contain drug-associated to polymer blends, dissolving after insertion into skin, allowing drug release. This study comprises development and characterization of a pyramidal model of dissolving MNs (500 μm) prepared with 5% wt/wt aminolevulinic acid and 20% wt/wt Gantrez AN-139 in aqueous blend. Protoporphyrin IX formation and distribution were evaluated in tumor mice model by using fluorescence widefield imaging, spectroscopy, and confocal microscopy. MNs demonstrated excellent mechanical resistance penetrating about 250 μm with minor size alteration in vitro, and fluorescence intensity was 5-times higher at 0.5 mm on average compared to cream in vivo (being 10 ± 5 a.u. for MNs and 2.4 ± 0.8 a.u. for cream). Dissolving MNs have overcome topical cream application, being extremely promising especially for thicker skin lesions treatment using PDT.

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Use of dermograph for improvement of PpIX precursor’s delivery in photodynamic therapy: Experimental and clinical pilot studies

Cited by 11 publications

References 25 publications

Strategies to Improve Drug Delivery in Topical PDT

Strategies to Improve Drug Delivery in Topical PDT

Photodynamic Therapy for Basal Cell Carcinoma: The Clinical Context for Future Research Priorities

Dissolving microneedles containing aminolevulinic acid improves protoporphyrin IX distribution

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