Use of Diagnostic Strains of Salmonella/Microsome Assay to Compare the Mutagenicity of Atmospheric Particulate Matter From Limeira and Stockholm

Abstract

The air particulate matter (PM) is recognized as mutagenic and this mutagenicity is mainly associated to polycyclic aromatic hydrocarbons (PAHs) and their derivatives, which can be more mutagenic to its precursors. The chemical composition of PM is related to climatic conditions such as temperature and solar radiation. Based on this, the PM samplings were performed in cities with different environmental conditions, in order to compare mutagenicity profiles and chemical composition of organic extracts. The samples chemical characterization was carried out using HPLC-GC/MS, and mutagenicity was evaluated using the Salmonella/microsome assay in microsuspension with differents diagnostic strains.