“…By using the H2O solution, it was possible to observe the low percentage of emergence (Table 1), thus presenting the need to use methods to overcome dormancy as the use of GA3 solution to break seed dormancy and achieve better results in emergence. Despite reports of studies on the low germination of annatto seeds (Lopes et al, 2008;Neto et al, 2018),many authors attribute its low germination to the impermeability of the tegument (Castello et al, 2012;Fernandes et al, 2021;Pereira et al, 2012),to which methods of overcoming dormancy can be assigned to improve seed viability. In this work, the treatment with GA3 solution for a period of 24 hours was efficient to overcome the dormancy imposed by the tegument of the annatto seed (Table 1), being possible in the initial stand test and the emergence velocity index to separate the solutions in levels of distinction with the GA3/0h and H20 solution lower and the GA3/24h higher and presenting a higher percentage of emergence compared with the other solutions, therefore as mentioned by authors due to the tegument constitute a physical barrier hindering the entry of water with the need for methods to overcome dormancy (Amaral et al, 2000;Custódio et al, 2002;Picolotto et al, 2013).Annatto seeds, when submitted to mechanical and sulfuric acid scarification, absorb water satisfactorily, overcoming the dormancy imposed by the tegument, and the use of GA3 also increases the speed of germination (Bocatto & Forti, 2020),thus evidencing the need for methods to overcome the dormancy of annatto seeds.…”