Use of Differential Rumen Fluid-Based Carbohydrate Agar Media for Culturing Lactose-Selected Cecal Bacteria from Chickens

Abstract

A rumen fluid-based differential carbohydrate agar medium for enumerating chicken cecal carbohydrate-utilizing bacteria was tested. Eleven bacteria isolated from a continuous-flow culture that had been seeded with chicken cecal contents were inoculated on fifteen different media in an anaerobic environment. These media included various levels of rumen fluid (0%, 5%, 10%, 16%) and carbohydrate (glucose [GLU], galactose [GAL], lactose[LAC]). Viable cell recovery (colony-forming units [CFU]/direct cell counts × 100%) was higher for 16% rumen fluid-supplemented carbohydrate media than the lower concentrations of rumen fluid-supplemented carbohydrate media. In addition, average viable cell recovery of all 11 bacteria on differential carbohydrate media were significantly (P < 0.05) higher in galactose and lactose media than on the glucose media. Data indicated that 16% rumen-fluid based media can be used to enumerate anaerobic, lactose-fermenting subgroups of chicken cecal bacteria.

Reduction of Salmonella Typhimurium by Fermentation Metabolites of Diamond V Original XPC in an In Vitro Anaerobic Mixed Chicken Cecal Culture

“…The ceca are the main site where pathogens including Salmonella colonize ( 30 ). Since poultry have a relatively slow digestion transit time, poultry ceca have a large number of bacteria, and the majority of these are strictly anaerobic ( 27 , 31 , 32 ). Cecal bacteria in poultry become more diverse as the host matures, and they can maximize metabolic fermentation in an anaerobic environment ( 12 ).…”

Caecal metabolites and microbial populations in chickens consuming diets containing a mined humate compound

“…tested a variety of di †erential carbohydrate agar media for enumeration of anaerobic, carbohydrate-fermenting subgroups of chicken caecal bacteria (Fan et al 1995). In the current study we expanded upon our previous experiments to design an optimal basal medium based on previous work by our group and others (Leedle and Hespell 1980 ;Barlaz et al 1989 ;Fan et al 1995 ;Maciorowski et al 1997).…”

“…In the current study we expanded upon our previous experiments to design an optimal basal medium based on previous work by our group and others (Leedle and Hespell 1980 ;Barlaz et al 1989 ;Fan et al 1995 ;Maciorowski et al 1997). MH did not signiÐcantly inÑuence any of the anaerobic microbial populations but signiÐ-cantly a †ected the population of the facultative microorganism Escherichia coli (Table 5).…”

Differential effects of rice bran cultivars to limit Salmonella Typhimurium in chicken cecal in vitro incubations and impact on the cecal microbiome and metabolome

“…The chicken cecal microbiota were inoculated from freshly harvested chicken ceca at a 1:3000 dilution into anaerobic dilution solution (ADS:,0.45g/L K 2 HPO 4 , 0.45 g/L KH 2 PO 4 , 0.45 g/L (NH 4 ) 2 SO 4 , 0.9 g/L NaCl, 0.1875 g/L MgSO 4 -7H 2 O, 0.12 g/L CaCl 2 -2H 2 O, 1 ml/L 0.1% resazurin, 0.05% cysteine-HCl, and 0.4% sodium carbonate) [ 16 , 24 – 28 ] with ground (25 mesh) Torres chick starter feed (1.25% w/v) as the carbon source. Prepared ADS was sparged with an anaerobic gas mixture consisting of 90% nitrogen/5% carbon dioxide/5% hydrogen in an anaerobic chamber for 30 minutes using an aquarium air pump and airstone before autoclaving.…”

“…The main site of Salmonella colonization in poultry is the ceca [ 13 ]. Poultry ceca contain a variety of bacteria which are characterized as strict anaerobes [ 14 – 16 ]. To investigate the possible effects of prebiotics or prebiotic-like materials on these anaerobic bacteria and their interaction with prebiotics and Salmonella , an anaerobic mixed culture system has been employed as a screening tool [ 17 ].…”