Use of dimethylsulfoxide for semen cryopreservation in Indian red jungle fowl (Gallus gallus murghi)

Rakha, Bushra Allah; Ansari, Muhammad Tayyab; Akhter, Shamim; Zafar, Zartasha; Naseer, Amir; Hussaın, Iftikhar; Blesbois, Élisabeth; Santiago-Moreno, J.

doi:10.1016/j.theriogenology.2018.09.003

Cited by 15 publications

(13 citation statements)

References 42 publications

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“…DMA and Me 2 SO are permeating cryoprotectant which act intra-and extracellularly inducing spermatozoa dehydration [41], osmotic stress [42], and even membrane fusion at high concentrations [43]. Despite this, the use of these cryoprotectants at concentrations of 6% of DMA and 8% of Me 2 SO resulted in high post-thaw plasma membrane integrity, viability, acrosomal integrity, motility and fertility in sperm from chicken, crane or Indian red jungle fowl [3,10,13]. The findings in the present study did not reveal differences between the two cryoprotectants.…”

Section: Discussionmentioning

confidence: 99%

“…Although the use of glycerol is associated in poultry with high sperm motility and viability after thawing, its use has been shown to have contraceptive effects in birds, and therefore glycerol must be eliminated from frozen-thawed semen before insemination [6,8,9]. Some alternatives to consider are the Me 2 SO (dimethyl sulphoxide) [10] or DMA (dimethylacetamide) [3,4]. The Me 2 SO is a permeable cryoprotectant that increases the fluidity lipid membrane's hydrophobic core (but a high concentration can disintegrate the lipid bilayer); enhances the permeability of hydrophilic molecules [11]; and decreases the ice crystallization [12].…”

Section: Introductionmentioning

confidence: 99%

“…The Me 2 SO is a permeable cryoprotectant that increases the fluidity lipid membrane's hydrophobic core (but a high concentration can disintegrate the lipid bilayer); enhances the permeability of hydrophilic molecules [11]; and decreases the ice crystallization [12]. The use of 8% Me 2 SO improved the frozen-thawed sperm quality and the fertility (73% fertility) in Indian red jungle fowl compared with other concentrations [10], and has been successfully used in crane sperm, obtaining a higher post-thaw survival and capacity to bind to inner perivitelline membrane than with DMA [13]. On the other hand, DMA has provided high levels of fertility after artificial insemination with frozen-thawed fowl (85-93% fertility) and sandhill crane (42-48% fertility) sperm [4,14,15].…”

Section: Introductionmentioning

confidence: 99%

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Sperm Cryopreservation in American Flamingo (Phoenicopterus Ruber): Influence of Cryoprotectants and Seminal Plasma Removal

Blanca

Martínez‐Nevado²,

Castaño

et al. 2021

Animals

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The American flamingo is a useful model for the development of successful semen cryopreservation procedures to be applied to threatened related species from the family Phoenicopteridae, and to permit genetic material banking. Current study sought to develop effective sperm cryopreservation protocols through examining the influences of two permeating cryoprotectants and the seminal plasma removal. During two consecutive years (April), semen samples were collected and frozen from American flamingos. In the first year, the effect of two permeating cryoprotectants, DMA (dimethylacetamide) (6%) or Me2SO (dimethylsulphoxide) (8%), on frozen–thawed sperm variables were compared in 21 males. No differences were seen between DMA and Me2SO for sperm motility, sperm viability, and DNA fragmentation after thawing. In the second year, the role of seminal plasma on sperm cryoresistance was investigated in 31 flamingos. Sperm samples were cryopreserved with and without seminal plasma, using Me2SO (8%) as a cryoprotectant. The results showed that samples with seminal plasma had higher values than samples without seminal plasma for the following sperm variables: Straight line velocity (22.40 µm/s vs. 16.64 µm/s), wobble (75.83% vs. 69.40%), (p < 0.05), linearity (62.73% vs. 52.01%) and straightness (82.38% vs. 73.79%) (p < 0.01); but acrosome integrity was lower (55.56% vs. 66.88%) (p < 0.05). The cryoresistance ratio (CR) was greater in samples frozen with seminal plasma than without seminal plasma for CR-progressive motility (138.72 vs. 54.59), CR-curvilinear velocity (105.98 vs. 89.32), CR-straight line velocity (152.77 vs. 112.58), CR-average path velocity (122.48 vs. 98.12), CR-wobble (111.75 vs. 102.04) (p < 0.05), CR-linearity (139.41 vs. 113.18), and CR-straightness (124.02 vs. 109.97) (p < 0.01). This research demonstrated that there were not differences between Me2SO and DMA to successful freezing sperm of flamingos; seminal plasma removal did not provide a benefit for sperm cryopreservation.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Sperm Cryopreservation in American Flamingo (Phoenicopterus Ruber): Influence of Cryoprotectants and Seminal Plasma Removal

Blanca

Martínez‐Nevado²,

Castaño

et al. 2021

Animals

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“…In domestic bird species, DMA is often recommended for sperm cryopreservation in pellets, and it yields better results when applied to protocols with rapid cooling rates [15,20,23]. In contrast, DMSO is better suited to slow freezing methods [24]. Additionally, low fertility outcomes have been observed when DMA/straw-based cryopreservation was compared with sperm cryopreserved with glycerol; this finding has been attributed to a negative interaction between the plastic straws and the cryoprotectant [23].…”

Section: Discussionmentioning

confidence: 99%

Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus)

Cardoso

Sánchez-Ajofrín

Castaño³

et al. 2020

Animals

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Simple Summary: The process of semen cryopreservation can have multiple advantages in an ex situ conservation program. However, there is a necessity to adapt the protocol to the specificity of each species. With that in mind, we aimed to optimize the sperm freezing/thawing process and study the effect of different cryoprotectants in the peregrine falcon.Abstract: Sperm cryopreservation is a complex process that needs to be adapted to wild and domestic avian species to ensure proper efficiency. Because of its accessibility, the peregrine falcon may be used as a good model for studying other raptor species. To find the most optimal cryopreservation protocol for peregrine falcon ejaculates, sperm parameters such as motility, viability, DNA fragmentation, acrosome integrity, and mitochondrial activity were analyzed under different conditions by varying the freezing method (slow freezing in straws vs. ultrarapid freezing in pellets), thawing conditions (37 • C for 30 s vs. 5 • C for 1 min), type of cryoprotectant (DMA vs. DMSO), and concentration of DMSO (4% vs. 8%). Results show that slow cryopreservation in straws yielded greater percentages (p < 0.05) of motile spermatozoa (22.5% ± 4.4% vs. 0.0% ± 4.1%), viable spermatozoa with intact acrosomes (84.6% ± 4.3% vs. 77.4% ± 4.3%), and spermatozoa with active mitochondria (41.0% ± 6.7% vs.12.8% ± 6.7%), compared with those obtained by the ultrarapid freezing in pellets. However, no differences were found between different thawing conditions. Moreover, all sperm motility parameters were greater (p < 0.05) when DMSO was used during freezing compared with DMA, although the use of 3% and 8% DMSO produced similar results. In conclusion, these results represent important progress in the study of falcon semen cryopreservation protocol, highlighting the crucial steps of the process and the most suitable conditions. Animals 2020, 10, 691 2 of 11 existent species. A viable presented solution is the creation of a germplasm bank that contains the sperm, oocytes, or even embryos of the largest possible number of species [2,3].Since the storage of eggs or embryos from birds poses a difficult challenge because of their large size and complexity, sperm cells are a good alternative [4]. Sperm is fairly easy and inexpensive to acquire, and its cryopreservation is much more accessible compared with the oocyte/embryo, because of the small volume of water inside of the sperm [5]. Furthermore, existing technologies allow the semen of many animal species to be collected and used [6]. Frozen semen can be a very important part of ex situ conservation efforts, since it allows for the storage of genetic material from multiple species for a long period of time. Stored semen can be used in reintroduction projects or to enlarge the genetic pool of a fragmented population. It also allows for the selection of individuals of genetic interest and the exchange of samples between distant locations [7,8]. In birds, frozen semen could become an increasingly relevant tool, because captive breeding programs a...

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“…Other methods including primordial germ cells and gonadic tissues technologies are still in progress but may be seen as methods that are complementary to semen cryopreservation (Liptoi et al., 2013; Nakamura, 2016; Sztan et al., 2017; Thélie et al., 2018). Different freezing procedures have been developed to cryopreserve avian semen (Blesbois et al., 2007; Santiago-Moreno et al., 2012; Rakha et al., 2016, 2017a; 2018a,b,c; Thananurak et al., 2016; Chuaychu-noo et al., 2017; Thananurak et al., 2017). However, simplification of the more efficient common methods of chicken sperm cryopreservation is still needed in order to make them available to large environmental and breeding conditions.…”

Section: Introductionmentioning

confidence: 99%

Sucrose increases the quality and fertilizing ability of cryopreserved chicken sperms in contrast to raffinose

Thananurak¹,

Chuaychu-noo²,

Thélie³

et al. 2019

Poultry Science

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Chicken semen conservation is an important tool for programs of genetic diversity management and of endangered breeds’ conservation. However, the method still needs to be improved in order to be applied in a wide variety of environments and breeds. Our objective was to compare the effects of 2 external cryoprotectants saccharides (sucrose and raffinose) on the sperm freezability of a Thai local breed, Pradu Hang Dum, in which semen was frozen with a simple freezing method using nitrogen vapors and dimethyl formamide (DMF). Thirty-six males were selected on their motility vigor score for the experiments. In a first experiment, a large range of sucrose and raffinose doses were tested. Semen quality was evaluated after incubation at 5°C or after cryopreservation in straws in the saline Blumberger Hahnen Sperma Verdünner diluent + DMF (6% v/v) with or without sucrose/raffinose. The best targeted doses of sucrose and raffinose were then kept for experiment 2 that was focused on cryopreserved semen. In this experiment, semen quality was measured on frozen-thawed sperm: different objective motility data evaluated by computer-assisted sperm analysis (CASA), membrane integrity, acrosome integrity, mitochondria function evaluated using flow cytometry, lipid peroxide production assessed by the thiobarbituric acid test. Fertility obtained with frozen-thawed semen supplemented or not with sucrose or raffinose was also evaluated after artificial insemination of laying hens. The presence of sucrose at the osmotically inactive dose 1 mmol significantly increased the vigor motility, membrane integrity, acrosome integrity, and mitochondrial functions of frozen-thawed sperm ( P < 0.05), and showed the highest levels of fertility after sperm cryopreservation (91% vs. control 86%, P < 0.001). Raffinose showed negative effects on in vitro semen quality from 1 to 100 mmol. Fertility was also negatively ( P < 0.001) affected by raffinose (fertility rate 66 to 70%). We thus showed in the present study the high success of a simple chicken sperm cryopreservation method with an external cryoprotectant easily available and cheap, the sucrose, used at an osmotically inactive low concentration.

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Use of dimethylsulfoxide for semen cryopreservation in Indian red jungle fowl (Gallus gallus murghi)

Cited by 15 publications

References 42 publications

Sperm Cryopreservation in American Flamingo (Phoenicopterus Ruber): Influence of Cryoprotectants and Seminal Plasma Removal

Sperm Cryopreservation in American Flamingo (Phoenicopterus Ruber): Influence of Cryoprotectants and Seminal Plasma Removal

Optimization of Sperm Cryopreservation Protocol for Peregrine Falcon (Falco peregrinus)

Sucrose increases the quality and fertilizing ability of cryopreserved chicken sperms in contrast to raffinose

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