Use of diphenylamine reagent for high-performance thin-layer chromatographic detection of organochloro insecticide endosulfan in biological samples

Current Strategies for Studying the Natural and Synthetic Bioactive Compounds in Food by Chromatographic Separation Techniques

“…Liquid chromatography, including thin-layer chromatography, along with other chromatographic techniques, is one of the most popular methods used in the current analysis of bioorganic and bioinorganic compounds in different including food samples [2][3][4][5][6][7][8][9][10][11][12][13][14][15].…”

“…Some studies indicate the important role of TLC and HPTLC methods as comprehensive techniques for the detection and identification of pesticides and the toxicity caused by these compounds [9][10][11][12][13][14][15]. Several new chromogenic reagents have been reported in the literature such as diphenylamine reagent for detection of organochloro insecticide endosulfan [9], stannous chloride and hydrochloric acid (reducing reagent) followed by a sodium nitrite in hydrochloric acid (coupling reagent) and β-napthol in sodium hydroxide for the detection of herbicide oxyfluorten [10], chloranil reagent with nitric acid for detection of organophosporus insecticide monocrotophos [11], 4-amminoantipyrene reagent with potassium ferricyanide for detection and identification of 2,4-dichlorophenol, an intermediate of 2,4-D (2,4-dichlorophenoxyacetic acid) herbicide [12], cupric acetate reagent for detection of organophosphate insecticide profenofos [13], and cobalt thiocyanate reagent for detection of organophosporus herbicide glyphosate [14]. Hussain et al [15] developed an HPTLC method for the determination of residues of various pesticides in brinjal samples from a market of Pakistan.…”