Use of Direct Latex Agglutination Testing of Selective Broth in the Detection of Group B Strepptococcal Carriage in Pregnant Women

Guerrero, Carmen; Martínez-Palomera, Jorge; Menasalvas, Ana; Blázquez, R.; Rodriguez, Tomas; Segovia, Manuel

doi:10.1007/s10096-003-1052-x

Cited by 18 publications

(4 citation statements)

References 6 publications

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“…Aiming the evaluation of the microbial identification tests, sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) were calculated, considering serogrouping as the gold standard[ 32 ].…”

Section: Methodsmentioning

confidence: 99%

Streptococcus agalactiae: Identification methods, antimicrobial susceptibility, and resistance genes in pregnant women

Santana¹,

Oliveira²,

Filho³

et al. 2020

WJCC

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BACKGROUND Group B Streptococcus (GBS) is a normal component of the gastrointestinal and genital microbiota in humans and can lead to important infections in newborns. AIM To compare GBS isolation and identification methods as well as to assess the antibiotic susceptibility and to identify resistance genes in GBS strains from pregnant women attended in healthcare services from the city of Vitória da Conquista, in Bahia State, Brazil. METHODS From January 2017 to February 2018, vaginorectal swabs were obtained from 186 participants and the samples were seeded onto chromogenic agar for GBS before and after inoculation in selective broth. Confirmatory identification using 3 CAMP and latex tests was performed in samples with GBS-suggestive colonies. Then, disk diffusion antibiograms were performed in GBS-positive samples, and the detection of the resistance genes erm B, erm TR, mef A, and lin B in the clindamycin and/or erythromycin-resistant samples was carried out. RESULTS Thirty-two samples (17.2%) were GBS-positive. The culture in chromogenic agar after sample incubation in selective broth was the most sensitive method (96.9%) for GBS detection. All isolates were susceptible to penicillin, ampicillin, cefotaxime, and vancomycin. Clindamycin resistance was observed in 6 samples (18.8%), while 8 samples (25%) were erythromycin-resistant. All erythromycin and/or clindamycin-resistant GBS strains had negative D-tests. Two strains (25%) presented an M phenotype and 6 isolates (75%) presented a cMLSB phenotype. The erm B gene was identified in 4 samples (44.4%), the mef A gene was also found in 4 samples (44.4%), the erm TR gene was identified in 1 isolate (11.1%), and the lin B gene was not found in any isolate. CONCLUSION This study evidenced that the screening for SGB can be performed by means of various methods, including chromogenic media, and that the chemoprophylaxis for pregnant women who cannot use penicillin must be susceptibility-guided.

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Section: Methodsmentioning

confidence: 99%

Streptococcus agalactiae: Identification methods, antimicrobial susceptibility, and resistance genes in pregnant women

Santana¹,

Oliveira²,

Filho³

et al. 2020

WJCC

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show abstract

“…The plates were observed 24 h after inoculation; if there was no suspected growth of GBS, then they were reincubated for an additional 24 h. Suspected GBS colonies were subjected to CAMP testing (31). The results for the CAMP-test-positive isolates were confirmed with the latex agglutination test (LAT) (Oxoid, Basingstoke, Hants, UK) (32). Confirmed GBS isolates were stored at Ϫ70°C in medium containing skim milk, tryptone, glucose, and glycerol until they were transported to the department of microbiology at Dhaka Shishu Hospital for serotyping.…”

Section: Methodsmentioning

confidence: 99%

Group B Streptococcus among Pregnant Women and Newborns in Mirzapur, Bangladesh: Colonization, Vertical Transmission, and Serotype Distribution

et al. 2017

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Group B streptococcus (GBS) infection is a leading cause of death among newborns in developed countries. Data on the burden of GBS in Asian countries are lacking. This study aimed to understand (i) the rate of maternal rectovaginal GBS carriage, (ii) the rate of vertical transmission of GBS, as determined by culturing ear, umbilicus, and nasal swabs, and (iii) the distribution of GBS serotypes. This prospective observational study was conducted between September 2012 and November 2013 at Kumudini Women's Medical College Hospital, a secondary-level hospital in Mirzapur, Bangladesh. The study enrolled pregnant women who visited the outpatient clinic for antenatal care (ANC) and/or delivered a child in the inpatient department of Kumudini Women's Medical College Hospital and the babies born to those mothers. 12% [20/172 isolates]). This study shows that Bangladesh has all of the ingredients for invasive GBS disease, including colonization of mothers by invasive serotypes and vertical transmission to babies.

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“…Following enrichment, the conventional means for identifying GBS is through isolation on subculture to blood agar plates and presumptive identification by the CAMP test [12] or serologic identification using latex agglutination with group B streptococcal antisera [13]. Often, many laboratories direct inoculate the sample on a solid agar medium upon receipt of the swab in the laboratory in order to speed the identification of GBS; however, this procedure should never be used as a substitute for a selective broth medium, because as many as 50% of women who are GBS carriers have false-negative culture results [14].…”

Section: Early-onset Neonatal Sepsismentioning

confidence: 99%

How Can the Microbiologist Help in Diagnosing Neonatal Sepsis?

Paolucci

Landini

Sambri

2012

International Journal of Pediatrics

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Neonatal sepsis can be classified into two subtypes depending upon whether the onset of symptoms is before 72 hours of life (early-onset neonatal sepsis—EONS) or later (late-onset neonatal sepsis—LONS). These definitions have contributed greatly to diagnosis and treatment by identifying which microorganisms are likely to be responsible for sepsis during these periods and the expected outcomes of infection. This paper focuses on the tools that microbiologist can offer to diagnose and eventually prevent neonatal sepsis. Here, we discuss the advantages and limitation of the blood culture, the actual gold standard for sepsis diagnosis. In addition, we examine the utility of molecular techniques in the diagnosis and management of neonatal sepsis.

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Use of Direct Latex Agglutination Testing of Selective Broth in the Detection of Group B Strepptococcal Carriage in Pregnant Women

Cited by 18 publications

References 6 publications

Streptococcus agalactiae: Identification methods, antimicrobial susceptibility, and resistance genes in pregnant women

Streptococcus agalactiae: Identification methods, antimicrobial susceptibility, and resistance genes in pregnant women

Group B Streptococcus among Pregnant Women and Newborns in Mirzapur, Bangladesh: Colonization, Vertical Transmission, and Serotype Distribution

How Can the Microbiologist Help in Diagnosing Neonatal Sepsis?

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