Use of Electrophoresis in the Identification and Quantitation of Antibiotics Administered in Combinations

Abstract

The investigation presents a method of electrophoretic separation of antibacterial drugs which are used in combinations in clinical medicine. Subsequent to electrophoresis in agarose gel, a microbiological assay was performed. This technique permitted the determination of the concentrations of beta-lactam antibiotics, rifampicin, and clindamycin in the presence of aminoglycosides. In therapeutic combinations of fusidic acid and clindamycin, the concentrations of each drug could be determined.

Rapid turbidimetric assay for quantification of fusidic acid in a dermatological cream

“…Several physicochemical methods have been described in the literature for the analysis of FA in different matrices, such as spectrophotometry [7][8][9], electrophoresis [10], electrospray ionization tandem mass spectrometry (ESI-MS) [11], atomic absorption spectroscopy [12], thin-layer chromatography [13][14][15], highperformance liquid chromatography [13,14,[16][17][18][19][20][21][22][23][24][25][26][27], and surfactant-dye binding degree method [28]. These methods are widely used to evaluate antimicrobial agents, and although precise, cannot represent the true biological activity of a drug.…”