Use of electrophoretic techniques to identify the proportion of an improved white clover cultivar (‘Grasslands Kopu’) in a mixed sward

Prins, E.; Sanders, P.; Wewala, G. S.; Lyons, T. B.

doi:10.1080/00288233.1989.10417925

Cited by 3 publications

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“…The study by ENNOS (1982) provides data on an esterase locus where the alleles segregate as for a diploid. Further studies of T. repens cultivars have, however, provided segregation patterns indicative of polyploid, disomic inheritance ( MICHAELSON-YEATES 1986;PRINS et al 1989;SAWADA and YAMAUCHI 1994;MARSHALL et al 1995). It is important to clarify the inheritance patterns of this polyploid species since the subsequent methodology and treatment in studies on breeding system, population structure and gene flow depend on this.…”

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Inheritance of the Isozyme Isocitrate Dehydrogenase (IDH) in a Natural Population of Polyploid White Clover (Trifolium Repens L.)

Weidema

2004

Hereditas

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An electrophoretic study of segregation of isocitrate dehydrogenase (IDH) was undertaken in polyploid white clover (Trifolium repem L.). The study aimed to test the conflicting theories on enzyme inheritance patterns in this species. A number of controlled selfings and reciprocal crosses were performed as well as a study of distribution of alleles in a natural Danish population of white clover. The result of the selfing experiment was that the chosen material is strongly self-incompatible, The offspring from the crosses showed that two isoloci were present and that the alleles in these segregate in a Mendelian fashion. Five different IDH-phenotypes, composed of two alleles, where found in the natural population. This contrasts with previous findings, for this and other allotetraploid species, of one variant and one invariant isolocus. Maximum likelihood analysis of the distribution of these phenotypes showed that the two alleles were equally common on an overall population basis. Each of these alleles were, however, most frequent in one of the isoloci. The findings reported here strongly support the theory that white clover is an allotetraploid, showing disomic inheritance.

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