Use of ensiled biomass sorghum increases ionic liquid pretreatment efficiency and reduces biofuel production cost and carbon footprint

Abstract

The use of ensiled biomass sorghum enables implementation of relatively mild pretreatment conditions compared to non-ensiled sorghum and results in higher sugar yields, which reduces the biofuel production cost and associated carbon footprint.

Genomics Characterization of an Engineered Corynebacterium glutamicum in Bioreactor Cultivation Under Ionic Liquid Stress

“…We also confirmed the ability of C. glutamicum BRC-JBEI 1.1.2 to handle renewable carbon streams from sorghum biomass using an improved carbon extraction protocol enhanced by the use of ensiled biomass ( Magurudeniya et al, 2021 ). The ensiling process enables naturally occurring lactic-acid secreting bacteria to partially decompose the hemicellulose in sorghum while stored in a silo before downstream processing.…”

Prediction of solubility parameters of lignin and ionic liquids using multi-resolution simulation approaches

“…The IL cholinium lysinate ([Ch][Lys]) was synthesized in our lab following published protocols. 61 The IL was purified before the experiments by freeze drier evaporation. A series of different organic solvents was purchased from Sigma Aldrich and used without any further purification.…”

Depolymerization of lignin for biological conversion through sulfonation and a chelator-mediated Fenton reaction

“…Culture samples (1.5 mL) were collected at the beginning of the experiment and after incubation at 30 °C with shaking for 24 h. The cells in these samples were harvested by centrifugation and the consumption of compounds in the supernatants was tracked using a previously reported HPLC method to detect sugars and acids. 40 For melanin analysis, the cell pellets were resuspended in 5 M NaOH containing 10% (v/v) of DMSO. The mixture was incubated for 1 h at 80 °C with 400 rpm shaking in a Thermomixer R (Eppendorf, Germany) and centrifuged at 3000 g for 3 minutes.…”