Use of enzyme-labelled protein G assay for the detection of anti Borrelia burgdorferi antibodies in wild animal sera

Deruaz, Dominique; Eid, Pierre; Deruaz, J.; Sempéré, A.; Bourgouin, Catherine; Rodhain, F; Perez-Eid, C.

doi:10.1007/bf00144006

Cited by 6 publications

(1 citation statement)

References 16 publications

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“…The affinity between protein-G and immunoglobulins from roe deer and red deer has been reported (Deruaz et al, 1996). A recent report stated that immunoglobulins from white-tailed deer and elk have approximately the same affinity to protein-G as cattle, and that protein-G does not necessarily bind immunoglobulins uniformly in closely related species (Kramsky et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Serologic Survey for Antibodies Against Mycobacterium Avium Subsp. Paratuberculosis in Free-Ranging Cervids From Norway

Tryland

Olsen

Vikøren

et al. 2004

Journal of Wildlife Diseases

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Affinity between protein-G and immunoglobulins from red deer (Cervus elaphus), moose (Alces alces), roe deer (Capreolus capreolus), and reindeer (Rangifer tarandus tarandus) was tested in a competition binding assay. Sera from red deer, reindeer, and moose inhibited the assay less than sera from cattle (less affinity), whereas sera from roe deer showed a slightly higher affinity to protein-G than did sera from cattle. The conclusion was made that protein-G could be used instead of anti-species antibodies for these cervid species, where the aim of the screening was to look for exposure or lack of exposure to mycobacteria in the tested populations. Serologic screening of 1,373 free-ranging cervids for antibodies against Mycobacterium avium subsp. paratuberculosis was conducted. All sera were tested by a protein-G-based antigen-absorbed enzyme-linked immunosorbent assay (ELISA). Seropositive moose (10/537; 1.9%), red deer (14/371; 3.8%), roe deer (6/49; 12.2%), and semidomesticated reindeer (11/325; 3.4%) were found, whereas wild reindeer (n = 91) were seronegative. In addition, the red deer sera were tested with a commercial ELISA, by which two animals tested positive and nine were suspicious of having M. avium subsp. paratuberculosis antibodies. Tissue samples and feces from 10 moose originating from a population with a clustering of seropositive animals were investigated by histology and bacteriology with negative results. Paratuberculosis has never been diagnosed in free-ranging or farmed cervid species in Norway. Thus, further studies are indicated to prove that the present findings reflect an infection with M. avium subsp. paratuberculosis.

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Section: Discussionmentioning

confidence: 99%