Use of eosin for green spectroscopic determination of mebendazole

Abstract

New, sensitive, and reliable spectroscopic methods were constructed for the fast determination of the anthelmintic drug mebendazole. The methods depended on the reaction of the amino group in mebendazole with eosin in acidic medium forming an ion pair complex. The first method, Method I, relied on quenching of the native fluorescence of eosin after reaction with mebendazole at pH 3.7 using acetate buffer. Fluorescence quenching was measured at 538 nm after excitation at 518 nm. This method showed a linear response over the concentration range 5.0–20.0 μg/ml. The second method, Method II, was based on measuring the absorbance of the formed complex at 554 nm; the method showed good linearity from 7.0 to 22.0 μg/ml. Different parameters that influenced the formation of the reaction product were carefully investigated to reach the optimized conditions. A comparison between the proposed methods and a previous spectrophotometric method was carried out and there was no significant difference between them. The methods could be applied successfully to determine mebendazole in its tablet form. Moreover, the methods used water as diluting solvent, which made them compatible with the ‘green’ analytical chemistry principles. No organic solvents were used throughout the study.

A selective dual quenching sensor (EY/BG@CDs) for simultaneous monitoring of gentamicin and ketorolac levels in plasma: a highly efficient platform that caters to the needs of therapeutic drug monitoring

“…Mechanisms of quenching involve either the interaction of the drug with EY through electron transfer, 26,27 or more commonly through ion-pair complexation between the drug and EY. [28][29][30] The use of EY as a sensing material has been demonstrated for the analysis of many drugs such as antibiotics, 31,32 beta-blockers, 28 and psychoactive drugs. 33 The advantages of using EY for the drug determination include high sensitivity, simplicity, cost effectiveness, and low detection limits.…”

Indirect Flow Injection Spectrophotometric and Chromatographic Methods for the Determination of Mebendazole in Pharmaceutical Formulations

“…Different analytical methods have been reported in the literature for the estimation of mebendazole either in pure form and formulations. These include HPLC [3][4][5] , spectrophotometry [6][7][8][9][10][11] , liquid chromatography-mass spectroscopy 12 and fluorimetry 13 . Most of the reported spectrophotometric methods depend on the formation of complexes which need an extraction step to get the formed colored product.…”

New spectrophotometric method for determination of mebendazole by oxidative coupling reaction

“…A survey of the literature showed that several spectrophotometric methods have been reported for the determination of MBL in pharmaceutical doses and biological samples. Most of these methods rely on several interactions between MBL and reagents to create a spectroscopically estimated color product for MBL, of which: (Eosin) (9) , (AgNPs) (10) , (Methanolic Hydrochloride) (11) , (N-Chloro Succinamide) (12) , (DNSA and CA) (13) , (Lanthanum (III)) (14) , (H2SO4 in methanol) (15) , (1-fluoro 2,4-dinitrobenzene) (16) .…”