Use of erythrocyte adhesion assay to predict the risk of diabetic complications

Isaji, Yayoi; Okochi, Mina; Horio, Fumihiko; Honda, Hiroyuki

doi:10.1016/j.bej.2008.09.016

Cited by 8 publications

(4 citation statements)

References 36 publications

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“…RBCs have an antioxidant defense system that is composed of the enzymes superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) and molecules, such as reduced glutathione (GSH), tierrodoxins, alpha tocopherol, beta carotene, and vitamin C [72]. The exposure of RBCs to methylmercury induces oxidative stress, which destabilizes the lipid bilayer of the cell membrane and causes morphological and physiological changes in the cell [63,73].…”

Section: Discussionmentioning

confidence: 99%

Mercury and Methylmercury Contamination of Terrestrial and Aquatic Ecosystems

2021

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Cover image courtesy of Stéphane Guédron© 2021 by the authors. Articles in this book are Open Access and distributed under the Creative Commons Attribution (CC BY) license, which allows users to download, copy and build upon published articles, as long as the author and publisher are properly credited, which ensures maximum dissemination and a wider impact of our publications.The book as a whole is distributed by MDPI under the terms and conditions of the Creative Commons license CC BY-NC-ND.

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Section: Discussionmentioning

confidence: 99%

Mercury and Methylmercury Contamination of Terrestrial and Aquatic Ecosystems

2021

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“…As a result, elasticity and fluidity are affected, as well as deformability and shape of red cells, which in turn influence the functional state of RBCs[ 42 ]. Since lipids are not synthesized in RBCs and the exchange is restricted between lipids and plasma[ 43 ], the damage is accumulated and eventually leads to either premature sequestration and RBCs elimination from the bloodstream or their lysis.…”

Section: Structural and Functional Disorders Of Blood Cells Under Dmmentioning

confidence: 99%

Effect of medicinal mushrooms on blood cells under conditions of diabetes mellitus

Vitak¹,

Yurkiv²,

Wasser³

et al. 2017

WJD

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Diabetes mellitus (DM) is the third most common non-infectious disease leading to early disability and high mortality. Moreover, the number of patients is growing every year. The main symptom of DM is hyperglycemia. Increased levels of blood glucose activate polyol, hexosamine, and protein kinase metabolic pathways cause the intensification of non-enzymatic glycosylation and nitration of macromolecules. This, in turn, leads to the development of oxidative and nitrative stresses and secondary complications, such as different kinds of micro- and macroangiopathies. Metabolic disorders caused by insulin deficiency in diabetes significantly impede the functioning of a homeostasis system, which change the physical, biochemical, morphological, and functional properties of blood cells. As a result, the oxygen-transport function of red blood cells (RBCs), rheological properties of the blood, and functions of immunocompetent cells as well as the process of apoptosis are primarily affected. Modern pharmacotherapy focuses on the search for new preparations that aim to decrease blood glucose levels. Undesirable side effects and adverse reactions caused by synthetic medicines led to the search and investigation of new preparations of natural origin. Medicinal mushrooms play an important role among such new preparations. They are a source of a large number of high- and low-molecular compounds with pronounced biological effects. Our investigations show pronounced hypoglycemic and anti-anemic action of submerged cultivated mycelium powder of medicinal mushrooms Agaricus brasiliensis (A. brasiliensis) and Ganoderma lucidum (G. lucidum) on streptozotocin-induced DM in rats. Also, we showed that mycelium powders have membrane protective properties as evidenced by the redistribution of RBC populations towards the growth of full functional cell numbers. Normalization of parameters of leukocyte formula and suppression of apoptosis of white blood cells in diabetic rats treated with A. brasiliensis and G. lucidum mycelia indicates pronounced positive effects of these strains of mushrooms. Thus, the use of medicinal mushrooms for treatment of DM and in prevention development of its secondary complications might be a new effective approach of this disease’s cure. This article is aimed at summarizing and analyzing the literature data and basic achievements concerning DM type 1 treatment using medicinal mushrooms and showing the results obtained in our research.

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“…Sialic acid is an important factor for maintenance of the surface electrical charge and stability of biological cellular system 7 . In hyperglycemic conditions, there is auto oxidation of glucose and non-enzymatic glycation of proteins, which are the main factors contributing to the production of reactive oxygen species (ROS) and there is excessive accumulation of oxidative stress within RBCs which reflects into cellular damage by generation of lipid hydro peroxides and detachment of sialic acid residues 6 . As a Similarly, Osmotic Fragility index is a measure of the resistance of red blood cells to lysis by osmotic stress and resembles RBC deformity associated with the electro kinetic properties of the membrane 8 .…”

Section: Introductionmentioning

confidence: 99%

“…Human RBCs contains about 95% of the glutathione which is responsible for scavenging reactive oxygen species 6 . Sialic acid is an important factor for maintenance of the surface electrical charge and stability of biological cellular system 7 .…”

Section: Introductionmentioning

confidence: 99%

Alterations in Zeta Potential and Osmotic Fragility of Red Blood Cells in Hyperglycemic Conditions

Gaikwad

Karemore

Avari

2018

PBJ

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The zeta potential is an electrokinetic property of red blood cells surface and in different disease conditions this property of the erythrocytes varies. The objective of the present study was to evaluate the alteration in zeta potential, lipid peroxidation and osmotic fragility of erythrocytes in hyperglycemic conditions. The zeta potential of the RBCs was measured using Zeta meter System 4.0. Lipid peroxidation, an indicator of tissue injury induced by reactive oxygen species was measured by the thiobarbituric acid assay (TBA). The percent haemolysis in terms of osmotic fragility was determined using UV-Vis spectrophotometer (JASCO). The mean erythrocytic ZP of the control group was found to be 22.13±0.2789 mV whereas, erythrocytic ZP for diabetes mellitus patients was found to be 8.559±0.4864 mV.Similarly, when erythrocytic ZP of control pregnant women was measured, and it was found to be 21.07±0.3393 mV which were slightly lower than a control group. Mean ZP of GDM patients was found to be 10.12±0.2294 mV which was significantly less than both control group and pregnant control group. Variations in zeta potential values were accompanied by increased osmotic fragility of RBCs. It was also observed from determination of lipid peroxidation of erythrocytes, that there was formation of higher concentration of malondialdehyde with the erythrocytes of hyperglycemic patients compared to control group.The findings suggest that the zeta potential value of erythrocytes can act as a key indicator for demonstration of increased oxidative stress.

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Use of erythrocyte adhesion assay to predict the risk of diabetic complications

Cited by 8 publications

References 36 publications

Mercury and Methylmercury Contamination of Terrestrial and Aquatic Ecosystems

Mercury and Methylmercury Contamination of Terrestrial and Aquatic Ecosystems

Effect of medicinal mushrooms on blood cells under conditions of diabetes mellitus

Alterations in Zeta Potential and Osmotic Fragility of Red Blood Cells in Hyperglycemic Conditions

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