Use of ESR, Mössbauer Spectroscopy, and Squid-Magnetometry for the Characterization of Magnetic Nanoparticles on the Base of Metal Iron and Its Implications in Vivo

Mykhaylyk, Olga; Razumov, O. N.; Dudchenko, A. K.; Pankratov, Yuriy; Dobrinsky, Eduard K.; Sosnitsky, V. N.; Bakai, E. A.

doi:10.1007/978-1-4757-6482-6_14

Cited by 8 publications

(3 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The spectra of organs from MNP injected animals exhibit a broad resonance signal centered at about g = 2.2 with a line width of about 1100 Gauss. The shape and field location of this signal are identical to those of the standard MNP suspensions and are consistent with ESR spectra of superparamagnetic iron oxide nanoparticles reported in the literature. , As seen by the representative control tissue spectra, the signal intensity contributions from all background sources is negligible compared to that of MNP containing tissues. The only visible signal, seen in the kidney control, is a low-intensity, narrow peak ( g = 2.0, line width p-p = 240 Gauss), which can be attributed to ferritin .…”

Section: Resultssupporting

confidence: 86%

Comparison of Electron Spin Resonance Spectroscopy and Inductively-Coupled Plasma Optical Emission Spectroscopy for Biodistribution Analysis of Iron-Oxide Nanoparticles

et al. 2010

View full text Add to dashboard Cite

Magnetic nanoparticles (MNP) have been widely studied for use in targeted drug delivery. Analysis of MNP biodistribution is essential to evaluating the success of targeting strategies and the potential for off-target toxicity. This work compared the applicability of inductively-coupled plasma optical emission spectroscopy (ICP-OES) and electron spin resonance (ESR) spectroscopy in assessing MNP biodistribution. Biodistribution was evaluated in 9L-glioma bearing rats administered with MNP (12-25 mg Fe/kg) under magnetic targeting. Ex vivo analysis of MNP in animal tissues was performed with both ICP-OES and ESR. A cryogenic method was developed to overcome the technical hurdle of loading tissue samples into ESR tubes. Comparison of results from the ICP-OES and ESR measurements revealed two distinct relationships for organs accumulating high or low levels of MNP. In organs with high MNP accumulation such as liver and spleen, data were strongly correlated (r = 0.97, 0.94 for liver and spleen, respectively), thus validating equivalency of the two methods in this high concentration range (> 1000 nmol Fe/g tissue). The two sets of measurements, however, differed significantly in organs with lower levels of MNP accumulation such as brain, kidney, and the tumor. Whereas ESR resolved MNP to 10-55 nmol Fe/g tissue, ICP-OES failed to detect MNP due to masking by endogenous iron. These findings suggest that ESR coupled to cryogenic sample handling is more robust than ICP-OES, attaining better sensitivity in analyses. Such advantages render ESR the method of choice for accurate profiling of MNP biodistribution across tissues with high variability in nanoparticle accumulation.

show abstract

Section: Resultssupporting

confidence: 86%

Comparison of Electron Spin Resonance Spectroscopy and Inductively-Coupled Plasma Optical Emission Spectroscopy for Biodistribution Analysis of Iron-Oxide Nanoparticles

et al. 2010

View full text Add to dashboard Cite

show abstract

“…The chelatable iron concentrations in tissue and blood samples were determined based on the incorporation of LMWI into the iron-desferrioxamine B (Df) complexes with a characteristic ESR spectrum ( fi g. 1 , curve b) upon treatment with Df solution [15,16] . The stored iron concentration in tissues was evaluated against the background of the wide magnetic resonance spectra in the g-factor range of 2.0-4.5 stipulated by ferritin and/or hemosiderin iron [17][18][19] ( fi g. 2 ). The relative methemoglobin (MtHb) iron concentration was estimated using an amplitude A CuEDTA sample of the same shape and size as above, prepared in a 1: 1 water-glycerin matrix, was used as a reference sample.…”

mentioning

confidence: 99%

“…To improve the accuracy of the double integration, the ESR lines were simulated as a sum of Lorentzian profi le derivatives after subtraction of the background signal, as shown in fi gure 1 , curves a and b. Calibration curves of the double integrated intensity versus iron concentration were obtained for each of the iron species. The tissue ferritin ESR spectra were calibrated against Mössbauer data [17] .The concentration of transferrin [Tf ] …”

mentioning

confidence: 99%

Dysregulation of Non-Heme Iron Metabolism in Glial Brain Tumors

Mykhaylyk¹,

Dudchenko²,

Cherchenko

et al. 2005

Med Princ Pract

View full text Add to dashboard Cite

Objective: To study iron exchange irregularities in experimental animals and patients with glial brain tumors to ascertain the role of the ‘iron component’ in glial brain tumor pathogenesis. Subject and Methods: A suspension of A.101.08 tumor cells was implanted in the cortex of the left-brain hemisphere of rats to model experimentally induced glial brain tumor. At 7 or 14 days after implantation, blood and tissue samples from the tumor, peritumoral tissue, and brain regions were taken for analysis. Blood and plasma samples were obtained from 23 patients as well as biopsy samples taken during tumor removal surgery. Electron resonance spectroscopy was used to determine the concentrations of transferrin iron, transferrin in whole blood and in blood cells, and chelatable and stored iron in the tissues of experimental animals and patients. Results: Hypoferremia was found in rats with both small and large glial brain tumors, whereas hyperferremia was found to be a characteristic of malignant glial brain tumors in humans. We identified statistically significant increases in stored and chelatable iron concentrations in the tumor and peritumoral brain tissue compared to the blood and the adjacent brain tissue (probably normal) in both human malignant glial brain tumors and in rat experimental glial brain tumors. Conclusions: These findings suggest that iron misregulation plays a part in glial brain tumor pathogenesis and this may provide a basis for understanding the association between glial brain tumors and epilepsy.

show abstract

Peculiarities of Nonheme Iron Metabolism Upon Experimental Modelling of Rat Glial Brain Tumour. Perspectives for Diagnosis and Treatment

Mykhaylyk

Dudchenko

Lebedev

et al. 2002

Trace Elements in Man and Animals 10

View full text Add to dashboard Cite

Use of ESR, Mössbauer Spectroscopy, and Squid-Magnetometry for the Characterization of Magnetic Nanoparticles on the Base of Metal Iron and Its Implications in Vivo

Cited by 8 publications

References 43 publications

Comparison of Electron Spin Resonance Spectroscopy and Inductively-Coupled Plasma Optical Emission Spectroscopy for Biodistribution Analysis of Iron-Oxide Nanoparticles

Comparison of Electron Spin Resonance Spectroscopy and Inductively-Coupled Plasma Optical Emission Spectroscopy for Biodistribution Analysis of Iron-Oxide Nanoparticles

Dysregulation of Non-Heme Iron Metabolism in Glial Brain Tumors

Peculiarities of Nonheme Iron Metabolism Upon Experimental Modelling of Rat Glial Brain Tumour. Perspectives for Diagnosis and Treatment

Contact Info

Product

Resources

About