Use of experimental design and effective mobility calculations to develop a method for the determination of antimicrobials by capillary electrophoresis

Mamani, Mónica Cecília Vargas; Amaya-Farfán, Jaime; Reyes, Felix G. R.; Silva, José Alberto Fracassi da; Rath, Susanne

doi:10.1016/j.talanta.2008.04.062

Cited by 35 publications

(13 citation statements)

References 24 publications

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“…A capillary zone electrophoresis (CZE) procedure was developed and validated for determination of 7 antimicrobials exploiting experimental design and calculations of effective mobility. 191 Results agreed with those attained by the HPLC procedure recommended by the United States Pharmacopoeia. Validation of a CZE procedure for the determination of fluoroquinolones was also carried out.…”

Section: Pharmaceuticalssupporting

confidence: 81%

Analytical chemistry in Brazil: healthy and growing

Rocha

Pereira-Filho²,

Nóbrega³

2009

J. Braz. Chem. Soc.

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A química brasileira está crescendo rapidamente, sendo um dos principais alicerces para o contínuo desenvolvimento do país. Inspirados no 15º Encontro Nacional de Química Analítica, ENQA, com um número recorde de participantes e comunicações, decidiu-se revisar os recentes desenvolvimentos em química analítica no Brasil. Foram escolhidas as mesmas áreas de aplicação adotadas para a organização temática do ENQA e selecionados artigos publicados por autores brasileiros em 7 periódicos de ampla penetração na área. Os dois periódicos científicos editados pela Sociedade Brasileira de Química também foram pesquisados. Esta revisão não é exaustiva, porém os trabalhos selecionados após 2008 mostram uma significativa visão geral da área. Pode-se concluir que a química analítica brasileira está saudável, em crescimento e certamente atenderá as demandas do país como uma potência emergida.Chemical sciences in Brazil are growing fast and being one of the main supports for the continuous development of the country. Inspired on the 15 th Brazilian Meeting on Analytical Chemistry, ENQA, with a record number of participants and communications, we have decided to review recent developments in analytical chemistry in Brazil. We have chosen the same application areas used for the thematic organization of the ENQA and have searched papers published by Brazilian authors in 7 major and general journals in analytical chemistry. The two scientific journals published by the Brazilian Chemical Society were also searched. This review is not comprehensive, but the selected papers published after 2008 shown an impressive overview of the area. We may conclude that analytical chemistry is healthy and growing in Brazilian scenario and certainly will attend the demands of Brazil as an emerged power.

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Section: Pharmaceuticalssupporting

confidence: 81%

Analytical chemistry in Brazil: healthy and growing

Rocha

Pereira-Filho²,

Nóbrega³

2009

J. Braz. Chem. Soc.

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“…10,12 Addition of MeOH changes the polarity and increases the viscosity of the buffer, thus modifying the electrophoretic mobility of the analytes. An increase in the MeOH concentration led to an increase in the migration times of the analytes because of the decrease of EOF, but also to an improvement of the separation efficiency.…”

Section: Optimization Of the Separation Methodsmentioning

confidence: 99%

“…CIP and NOR exhibit very close values for both pKa1 and pKa2 due to structural similiarities, while the pKa values for OFL are smaller due to its tricyclic structure and substitution on the piperazine ring. 10 The pKa values of the three compounds are presented in Table 1. 4,5,8 Initial runs were carried out to assess the feasibility of borate and phosphate buffers as background electrolytes.…”

Section: Preliminary Studymentioning

confidence: 99%

Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatography

Hancu¹,

Rusu²,

Simon³

et al. 2012

J. Braz. Chem. Soc.

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Este trabalho descreve o desenvolvimento de um método simples de eletroforese capilar (EC) para determinação simultânea dos três derivados mais frequentemente usados da fluoroquinolona: ciprofloxacina, norfloxacina e ofloxacina. Devido às características estruturais e físico-químicas muito semelhantes, ciprofloxacina e norfloxacina não podem ser separadas pelo método clássico de eletroforese capilar de zona (CZE). Suas separações podem ser resolvidas usando cromatografia eletrocinética micelar (MEKC), adicionando um surfactante aniônico à solução-tampão. A eficiência da separação pode ser intensificada através da otimização de parâmetros analíticos. Um método rápido e confiável foi desenvolvido, e os melhores resultados foram obtidos usando uma solução-tampão contendo tetraborato de sódio 25 mmol L -1 , dodecilsulfato de sódio 100 mmol L -1 e metanol 5% como modificador orgânico.This work describes the development of a simple capillary electrophoresis (CE) method for simultaneous determination of the three most frequently used fluoroquinolone derivatives: ciprofloxacin, norfloxacin and ofloxacin. Due to very similar structural and physicochemical characteristics, ciprofloxacin and norfloxacin cannot be separated by the classic method of capillary zone electrophoresis (CZE). Their separations can be solved using micellar electrokinetic chromatography (MEKC), by adding an anionic surfactant to the buffer solution. The efficiency of the separation can be enhanced through the optimization of analytical parameters. A fast reliable method was developed, and the best results were obtained using a buffer solution containing 25 mmol L -1 sodium tetraborate, 100 mmol L -1 sodium dodecyl sulphate and 5% methanol as organic modifier.

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“…Experimental design and effective mobility calculations are used by Mamani et al [45] to develop a multiclass method for quality control. Real samples of pharmaceutical formulations containing SMZ (tablets and an oral suspension) were analyzed using CZE.…”

Section: Czementioning

confidence: 99%

Analysis of sulfonamides by capillary electrophoresis

Hoff¹,

Kist²

2009

J of Separation Science

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The methods of analysis of sulfonamides (SFAs) using CE are reviewed. Sulfonamides were the first antimicrobial group of drugs used in medical treatment. These compounds are still used today in medicine and widely are employed in veterinary medicine, also for their growth promoter effects. Improved methods of analysis of sulfonamides are a constant challenge for researchers. CE is a new trend in the fields of pharmaceutical and food analyses. Several methods for the determination of SFAs by CE have been published in recent years, and the present review considers applications in quality control of pharmaceutical dosage forms, food analysis, determinations in serum, and other biological fluids as well as in electrophoresis experiments which examine the behavior of this class of compounds for theoretical studies of the technique. This review covers studies ranging from the pioneering works on sulfonamide analyses using classical electrophoresis to the more recent CE methods coupled to tandem mass spectrometers. The sections are divided following the EC modes like CZE, MEKC, and hyphenated methods (CE-MS, CE-MS/MS). A brief compilation of theoretical findings of sulfonamides electrophoretic behavior is also included. Parameters such as recoveries, LOD and LOQ, among others, are examined, covering works published until August 2008. This review can contribute to further research aimed to improve the analysis of SFAs by CE.

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Use of experimental design and effective mobility calculations to develop a method for the determination of antimicrobials by capillary electrophoresis

Cited by 35 publications

References 24 publications

Analytical chemistry in Brazil: healthy and growing

Analytical chemistry in Brazil: healthy and growing

Simultaneous separation of ciprofloxacin, norfloxacin and ofloxacin by micellar electrokinetic chromatography

Analysis of sulfonamides by capillary electrophoresis

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