Use of expressed sequence tag microsatellite markers for exploring genetic diversity in lentil and related wild species

Singh, Anand Kumar; Dikshit, Harsh Kumar; Singh, Dharmendra; Jain, Neelu; Aski, Muraleedhar S.; Sarker, Ashutosh; Sharma, Tilak Raj

doi:10.1017/s0021859615001252

Cited by 8 publications

(2 citation statements)

References 94 publications

(118 reference statements)

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“…Subsequently, the use of RFLP markers remained limited in lentil because it required high technical skills for their development. The advent of markers based on PCR (random amplified polymorphic DNA [RAPD], sequence characterized amplified region [SCAR], and SSR) has accelerated their use widely in lentil breeding programs (Kumar et al, 2015), and they are still in use (A. Singh et al, 2016; Ates, Aldemir, Alsaleh, et al, 2018; D. Singh et al, 2016; D. Singh et al, 2019; Gupta et al, 2016; Kumar, Choudhary, et al, 2018; Kumar, Gupta, Biradar, et al, 2018; Mbasani‐Mansi et al, 2019; Polanco et al, 2019; Tsanakas et al, 2018). In lentil, a set of 122 genomic SSR markers were developed for utilization in breeding programs (Verma et al, 2014).…”

Section: Acceleration In the Development Of Genomic Resources For Elevating Lentil From Orphan Statusmentioning

confidence: 99%

Genomics‐assisted lentil breeding: Current status and future strategies

et al. 2021

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Genomics‐assisted breeding has become a powerful tool to develop high‐yielding climate‐resilient varieties for adaptation to adverse environmental conditions such as heat and drought stresses. Previously, efforts have been made to develop genomic resources in lentil, leading to the development of many trait‐specific mapping populations, cores and mini‐cores, and single nucleotide polymorphism and simple sequence repeat markers. Molecular markers have been used in genetic diversity analyses and to clarify genetic relationships in lentil. However, availability of cost‐effective next‐generation sequencing and genotyping‐by‐sequencing technologies has provided unprecedented opportunities for advancing genetics research and breeding applications. For instance, it has become possible to assemble the large and complex genome, develop high‐density genetic maps for high‐resolution QTL mapping, and deploy genome‐wide association study in lentil. Furthermore, a range of cost‐effective marker genotyping platforms have been developed. These developments offer ample opportunities to modernize current breeding programs in lentil for accelerating genetic gains. This review discusses the current status and future possibilities of genomics‐assisted breeding to develop and deploy lentil cultivars suitable for changing climatic conditions.

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Section: Acceleration In the Development Of Genomic Resources For Elevating Lentil From Orphan Statusmentioning

confidence: 99%

Genomics‐assisted lentil breeding: Current status and future strategies

et al. 2021

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“…DNA markers could be developed from whole nuclear genome or expressed sequence tags (ESTs) (Singh et al, 2016). ESTs are short cDNA sequences reversely transcribed from mRNA.…”

Section: Expressed Sequence Tags (Ests)mentioning

confidence: 99%

Molecular markers for analysis of plant genetic diversity

Nam¹,

Hang²,

Linh³

et al. 2021

Vietnam J. Biotechnol.

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Genetic diversity plays an important role in diversity conservation at multiple levels and supports to monitor and assess genetic variation. In plants, genetic diversity provides the ability to adapt and respond to environmental conditions that helps plants to survive through changing environments. Genetic diversity analyses based on molecular genetic markers are effective tools for conservation and reintroduction of rare and endangered species. In recent years, the development of various chemical and molecular techniques for studying genetic diversity has received great attention. While biochemical markers are primarily used in the diagnosis of pathogens, DNA markers have been developed and widely applied for identification of species and population based on the genotype of an organism that is more stable and not easily affected by the environmental factors. PCR-based molecular marker tools, such as restriction fragment length polymorphisms (RFLPs), random amplified polymorphic DNA (RAPD), simple sequence repeats (SSRs) are used for analysing the difference in the targeted DNA sequences. With the rapid and robust development of genomic sequencing technology it is now possible to obtain and analyse DNA sequences of the whole genome of studied organisms. However, each type of DNA markers has different principles, as well as the pros and cons of specificity. In this article, we review methods and point out DNA markers, which are considered as reliable and widely used tools for the detection of genetic variation. In addition, we present the application of DNA marker in analysing genetic diversity of wild, domestic and medicinal plants, as well as some perspectives on the future of DNA marker’s application in the analysis of genetic diversity.

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