Use of extracellular polysaccharides, secreted by Lactobacillus plantarum and Bacillus spp., as reducing indole production agents to control biofilm formation and efflux pumps inhibitor in Escherichia coli

Mahdhi, Abdelkarim; Leban, Nadia; Chakroun, Ibtissem; Bayar, Sihem; Mahdouani, Kacem; Majdoub, Hatem; Kouidhi, Bochra

doi:10.1016/j.micpath.2018.10.010

Cited by 41 publications

(24 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…During the past few decades, lactic acid bacteria (LAB), a popular member of probiotics, have been extensively used in humans and animals for various purposes to enhance nutrient utilization, to modulate both the innate and the adaptive immune systems, and to inhibit the growth of numerous pathogenic microorganisms (12,13). Besides, LAB strains have been shown to limit the emergence of bacterial resistance by inhibiting the horizontal transmission of resistance genes (14,15) and biofilm production (16,17). The LAB produces several active metabolites, including organic acids, bacteriocins, hydrogen peroxides, exopolysaccharides, and biosurfactants, all of which may prevent the formation of biofilms (9).…”

Section: Introductionmentioning

confidence: 99%

Anticonjugation and Antibiofilm Evaluation of Probiotic Strains Lactobacillus plantarum 22F, 25F, and Pediococcus acidilactici 72N Against Escherichia coli Harboring mcr-1 Gene

et al. 2021

View full text Add to dashboard Cite

Several species of lactic acid bacteria (LAB) are commonly used as probiotics and as an alternative to antibiotics in various industries, especially in the livestock industry. This study aimed to investigate the anticonjugation and antibiofilm activity of cell-free supernatant (CFS) of Thai LAB strains (Lactobacillus plantarum 22F, 25F, and Pediococcus acidilactici 72N) against colistin-resistant Escherichia coli isolates. A total of six colistin-resistant E. coli strains were isolated from different sources, including pigs, farmers, and farmhouse environments. The E. coli were characterized by plasmid profiling, PCR detection of mcr-1 gene, and antibiotic susceptibility patterns. The CFS at dilutions ≥1:16 was chosen as the proper dilution for anticonjugation assay. Besides, it could significantly reduce the transfer frequencies of resistance gene mcr-1 up to 100 times compared to the neutralizing CFS (pH 6.5). The biofilm production in the planktonic stage was reduced by non-neutralizing and neutralizing CFS determining with crystal violet staining assay up to 82 and 60%, respectively. Moreover, the non-neutralizing CFS also inhibited the biofilm formation in the sessile stage up to 52%. The biofilm illustration was confirmed by scanning electron microscopy (SEM). These results agreed with the findings of the crystal violet technique, which showed a significant reduction in cell density, aggregation, and extracellular polysaccharide (EPS) matrix. The application of Thai LAB may serve as an attractive alternative to antibiotics for reducing biofilm formation and limiting the proliferation of antibiotic-resistant genes.

show abstract

Section: Introductionmentioning

confidence: 99%

Anticonjugation and Antibiofilm Evaluation of Probiotic Strains Lactobacillus plantarum 22F, 25F, and Pediococcus acidilactici 72N Against Escherichia coli Harboring mcr-1 Gene

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Several polysaccharides have been reported to inhibit biofilm formation (Mahdhi et al . 2018; Vishwakarma and Vavilala 2019), but seldom research was done to investigate the underlying molecular mechanism on transcriptomic level. In our study, the anti‐biofilm and anti‐infection mechanism of exopolysaccharide EPS273 against P. aeruginosa PAO1 was analysed on transcriptional levels.…”

Section: Discussionmentioning

confidence: 99%

“…Normally, polysaccharide is a major component of biofilm and its production is an important feature of mature biofilm, while more and more bacterial polysaccharides have been reported recently to negatively regulated biofilm formation (Mahdhi et al . 2018; Vishwakarma and Vavilala 2019). Exopolysaccharide EPS273 with the average molecular weight about 190 kDa, which was isolated from a marine bacterium P. stutzeri 273, primarily consists of glucosamine, rhamnose, glucose and mannose.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional profiling of Pseudomonas aeruginosa PAO1 in response to anti‐biofilm and anti‐infection agent exopolysaccharide EPS273

Zheng

Zhang

et al. 2020

J. Appl. Microbiol.

View full text Add to dashboard Cite

Aims: Relatively, few anti-biofilm polysaccharides against Pseudomonas aeruginosa were done to investigate the underlying molecular mechanism. Exopolysaccharide EPS273 can clearly reduce biofilm formation and infection of P. aeruginosa. This study aims to investigate its anti-biofilm and antiinfection mechanism on transcriptional level. Methods and Results: Herein, we used an RNA-Seq transcriptomic approach to investigate the underlying anti-biofilm and anti-infection mechanism of EPS273. The expression levels of a large number of genes were changed after P. aeruginosa PAO1 was treated with EPS273. Especially, the genes related to biofilm formation, such as gene involved in production of extracellular matrix and virulence factor, genes involved in flagella and cell motility and genes involved in iron acquisition. Notably, the expression levels of genes involved in regulatory and signal transduction were markedly downregulated, such as twocomponent system PhoP-PhoQ and quorum sensing (QS) system LasI/LasR and RhlI/RhlR. Furthermore, when genes phoP and phoQ were disrupted, respectively, the reduction of biofilm formation and cell motility in mutant MphoP or MphoQ was also detected. Conclusion: EPS273 may exert its anti-biofilm and anti-infection function by downregulating gene expression of two-component system PhoP-PhoQ and QS systems LasI/LasR and RhlI/RhlR of P. aeruginosa, which further regulated expression of genes involved in biofilm formation. Significance and Impact of the Study: Our data will expand understanding of anti-biofilm mechanisms of polysaccharides on transcriptomic level. and have been implicated in numerous chronic and persistent infections by forming biofilms (Gonzalez et al. 2018). The biofilm cells have been shown to be 10-to 1000-fold less susceptible to various antimicrobial agent than the planktonic cells, thus causing great difficulties in eradicating them completely (Taylor et al. 2014). Due to the extraordinary resistance of biofilm cells, inhibiting of biofilm formation becomes an increasingly explored alternative anti-pathogenic approach (Zhou et al. 2017; Bidossi et al. 2019). Biofilms are communities of surface-associated bacteria embedded in a self-produced, hydrated polymeric matrix,

show abstract

“…Some of these mechanisms include (a) the production of antagonistic compounds, (b) competition with pathogens and (d) modulation of host immune responses [ 118 ] ( Figure 2 ). Probiotics can produce various antagonistic compounds, including exopolysaccharides [ 119 ], bacteriocins [ 120 ] and biosurfactants [ 121 ] which exhibit antibiofilm activity. These antagonistic compounds have been shown to interfere with biofilm attachment and formation as well as the thinning of mature biofilms.…”

Section: Strategies To Target Gut Microbial Biofilmsmentioning

confidence: 99%

Targeting Gut Microbial Biofilms—A Key to Hinder Colon Carcinogenesis?

Chew

Tan

Law

et al. 2020

Cancers

View full text Add to dashboard Cite

Colorectal cancer (CRC) is a global public health issue which poses a substantial humanistic and economic burden on patients, healthcare systems and society. In recent years, intestinal dysbiosis has been suggested to be involved in the pathogenesis of CRC, with specific pathogens exhibiting oncogenic potentials such as Fusobacterium nucleatum, Escherichia coli and enterotoxigenic Bacteroides fragilis having been found to contribute to CRC development. More recently, it has been shown that initiation of CRC development by these microorganisms requires the formation of biofilms. Gut microbial biofilm forms in the inner colonic mucus layer and is composed of polymicrobial communities. Biofilm results in the redistribution of colonic epithelial cell E-cadherin, increases permeability of the gut and causes a loss of function of the intestinal barrier, all of which enhance intestinal dysbiosis. This literature review aims to compile the various strategies that target these pathogenic biofilms and could potentially play a role in the prevention of CRC. We explore the potential use of natural products, silver nanoparticles, upconverting nanoparticles, thiosalicylate complexes, anti-rheumatic agent (Auranofin), probiotics and quorum-sensing inhibitors as strategies to hinder colon carcinogenesis via targeting colon-associated biofilms.

show abstract

Use of extracellular polysaccharides, secreted by Lactobacillus plantarum and Bacillus spp., as reducing indole production agents to control biofilm formation and efflux pumps inhibitor in Escherichia coli

Cited by 41 publications

References 34 publications

Anticonjugation and Antibiofilm Evaluation of Probiotic Strains Lactobacillus plantarum 22F, 25F, and Pediococcus acidilactici 72N Against Escherichia coli Harboring mcr-1 Gene

Anticonjugation and Antibiofilm Evaluation of Probiotic Strains Lactobacillus plantarum 22F, 25F, and Pediococcus acidilactici 72N Against Escherichia coli Harboring mcr-1 Gene

Transcriptional profiling of Pseudomonas aeruginosa PAO1 in response to anti‐biofilm and anti‐infection agent exopolysaccharide EPS273

Targeting Gut Microbial Biofilms—A Key to Hinder Colon Carcinogenesis?

Contact Info

Product

Resources

About