Use of extracts from oyster shell and soil for cultivation of Spirulina maxima

Abstract

Calcium ion and trace metals play important roles in various metabolisms of photosynthetic organisms. In this study, simple methods were developed to extract calcium ion and micronutrients from oyster shell and common soil, and the prepared extracts were tested as a replacement of the corresponding chemicals that are essential for growth of microalgae. The oyster shell and soil were treated with 0.1 M sodium hydroxide or with 10 % hydrogen peroxide, respectively. The potential application of these natural sources to cultivation was investigated with Spirulina maxima. When compared to standard Zarrouk medium, the Spirulina maxima cultivated in a modified Zarrouk media with elements from oyster shell and soil extract exhibited increases in biomass, chlorophyll, and phycocyanin by 17, 16, and 64 %, respectively. These results indicate that the extracts of oyster shell and soil provide sufficient amounts of calcium and trace metals for successful cultivation of Spirulina maxima.

Use of the effluent from biogas production for cultivation of Spirulina

“…Phycocyanin has a role as an accessory pigment and increased production could have been a physiological response to the lower amount of light. Similarly, Jung et al [23] who used extracts from oyster shells and soil as an amendment to Zarrouk’s medium during cultivation of Spirulina ( A. maxima ) observed a significant increase in phycocyanin concentration when the extract was added.…”

Enhanced phycocyanin production of Arthrospira maxima by addition of mineral elements and polypeptides using response surface methodology

“…Mostly researches showed that the addition of mineral components can promote the phycocyanin accumulation. Compared to the control (Zarrouk medium), phycocyanin concentration increased by 1.6 times when the modified Zarrouk medium containing more Ca 2+ from oyster shell and soil extract was used (Jung et al, 2014). A low concentration (<0.3 mg mL -1 ) of Ni increased the content of phycocyanin in Microcystis aeruginosa (Liu et al, 2005).…”

“…Besides, the addition of exogenous substances is also an efficient approach to promote the biomass and phycocyanin accumulation. The supplements of mineral elements, organic acids, and vitamins in culture medium have been reported to enhance biomass and phycocyanin production (Jung et al, 2014;Bachchhav et al, 2016;Mogany et al, 2018). Stepwise addition of selenium (Se) during the exponential growth phase of Arthrospira.…”

Utilização do resíduo de malte da indústria cervejeira como meio alternativo para o cultivo da Spirulina platensis e Spirulina máxima

“…Valores similares foram encontrados por Ilter et al (2018), com 74,7 mg g -1 e por Prates et al (2018) com 83,7 mg g -1 de ficocianina da Spirulina platensis em meio Zarrouk. Vale salientar que vários estudos têm utilizado eficientemente meios alternativos para produção de ficocianina como resíduos de alimentos de restaurantes ou padarias (Sloth et al, 2017), água residual do cultivo de camarão (Sandeep et al, 2015), extratos de broto de feijão (Dianursanti et al, 2018) extratos de conchas de ostras e solo (Jung et al, 2014). Evidenciando a eficiência dos meios alternativos já que o crescimento e a produção de ficocianina de Spirulina platensis são altamente dependentes da disponibilidade de nutrientes no meio de cultura (Amanatin et al, 2013).…”