Use of fibrin glue derived from snake venom in the repair of deep corneal ulcers: experimental study in dogs (Canis familiaris, Linnaeus, 1758)

Abstract

ABSTRACT:Fibrin glue has been researched as an alternative method for tissue synthesis and is known for its capability to promote hemostasis at the application site, good approximation of wound edges and fast healing. The current study consisted in the application of fibrin glue derived from snake venom as treatment for experimental corneal ulcers. Twenty-one dogs had their corneas experimentally prepared through lamellar keratectomy (of standardized diameter and depth). Animals were divided into seven groups of three animals each. Six experimental groups were periodically evaluated and collection was carried out on the 1 st , 3 rd , 7 th , 15 th , 30 th and 60 th post-operative days, whereas one control group was evaluated throughout the experiment. Analyses consisted in the clinical evolution and in the histopathological study of all operated on eyes. Results indicated that fibrin glue was efficient in repairing keratectomy wounds in dogs and contributed to an earlier healing phenomenon, avoiding edema formation and keeping corneal clearness. The use of fibrin glue derived from snake venom showed to be easy to apply, feasible with animal models and of low cost, avoiding the lesion progress and allowing fast and appropriate corneal healing.

Unique heterologous fibrin biopolymer with hemostatic, adhesive, sealant, scaffold and drug delivery properties: a systematic review

“…Thus, fibrin sealant derived from snake venom can be used to promote healing in the flexor tendon of dogs.None informed. Sampaio et al (2007) [46] To elucidate mechanisms related to the repair of corneal ulcers treated with the fibrin sealant derived from snake venom.Twenty-one dogs were submitted to lamellar keratectomy divided into seven groups (n = 31). G1 to G6: keratectomized areas with fibrin sealant (collection performed on days 1, 3, 7, 15, 30, 60 after surgery); G7: without sealant.The corneas collected at day 60 presented discrete changes compared to day 30 in postoperative group.…”

“…Sampaio et al [46], proposed a model to repair corneal ulcers in dogs. Although fibrinolysis occurred within the first 48 hours, the healing of the eyes treated with fibrin sealant did not present complications.…”

Hydroxyapatite and a New Fibrin Sealant Derived from Snake Venom as Scaffold to Treatment of Cranial Defects in Rats

“…In addition, patients may develop antibodies against bovine thrombin 13 . In an attempt to overcome these difficulties, another sealant consisting of a thrombin-like enzyme extracted from snake venom and fibrinogen obtained from large animals to replace human components has shown excellent results in experimental studies on the regeneration of different tissues [14][15][16][17][18] , in mesenchymal cell cultures 19 , and in the healing of skin wound, venous ulcers and gingival graft sutures in humans 20,21 . This new sealant is a biodegradable product that does not cause adverse reactions, does not contain human blood components, has good adhesive capacity, and does not transmit infectious diseases 22 .…”

Chronic venous ulcers: a review on treatment with fibrin sealant and prognostic advances using proteomic strategies

“…In addition to these studies, the heterologous fibrin sealant was tested experimentally on rodents [38,39,40,41,42,43,44] dogs [45,46,47,48] and sheep [49,50], as detailed in a recent systematic review by Buchaim et al [37].…”