Use of flow cytometric immunophenotyping to refine the cytological diagnosis of canine lymphoma

Comazzi, S.; Gelain, Maria Elena

doi:10.1016/j.tvjl.2010.03.011

Cited by 66 publications

(100 citation statements)

References 44 publications

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“…The annual rate of occurrence varies between 6 and 30 cases per 100,000 dogs (Vonderhaar and Morrison, 2002;Morrison, 2004). Lymphoma accounts for approximately 7% up to 24% of all canine neoplasms and about 83% of all malignant haematopoietic tumors of this species (Valli et al, 2017;Vail et al, 2013 Comazzi and Gelain, 2011). The immunophenotype of a lymphocyte is identified by determining the expression of specific molecules for B lymphocytes (e.g., CD79a, CD20, CD21), and T lymphocytes (e.g., CD3) (Ponce et al, 2003;Vail et al, 2013).…”

Section: Introductionmentioning

confidence: 99%

Immunohistochemical Characterization of Canine Lymphomas

Cora¹,

Gal²,

Taulescu³

et al. 2017

BUASVMCN-VM

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Lymphomas occur by clonal expansion of lymphoid cells and have distinctive morphological and immunophenotypic features. Determination of canine lymphoma immunophenotype is useful for accurate prognosis and further therapy. In the suggested study, we performed an immunohistochemical evaluation of some cases with canine lymphoma diagnosed in the Department of Pathology (Faculty of Veterinary Medicine, ClujNapoca, Romania), in order to characterize them. The investigation included 39 dogs diagnosed with different anatomical forms of lymphoma, following necropsy analysis or assessment of biopsies. The diagnosis of lymphoma was confirmed by necropsy and histopathology (Hematoxylin-eosin stain) examinations. The collected specimens were analyzed by immunohistochemistry technique (automatic method) using the following antibodies: CD3, CD20, CD21 and CD79a. The analyzed neoplasms were characterized as follows: about 64.10% of cases were diagnosed as B-cell lymphomas, 33.34% of cases as T-cell lymphomas, whereas 2.56% of cases were null cell type lymphomas (neither B nor T). Most of multicentric (80%), mediastinal (60%) and primary central nervous system lymphomas (100%) had B immunophenotype, while the majority of cutaneous (80%) and digestive (100%) lymphomas had T immunophenotype. Immunohistochemical description of canine lymphomas can deliver some major details concerning their behavior and malignancy. Additionally, vital prognosis and efficacy of some therapeutic protocols are relying on the immunohistochemical features of canine lymphoma.

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Section: Introductionmentioning

confidence: 99%

Immunohistochemical Characterization of Canine Lymphomas

Cora¹,

Gal²,

Taulescu³

et al. 2017

BUASVMCN-VM

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“…Immunophenotyping increases the accuracy of diagnostics and sub-classification of most lymphoma subtypes (Comazzi and Gelain 2011). The immunophenotype is also a well-established prognostic marker for canine lymphoma (Dobson et al 2001), and FC is a suitable method for immunophenotypic analysis (Borska et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Gastrointestinal B-lymphoblastic lymphoma in a dog: a case report

Borska¹,

Husník²,

Fictum³

et al. 2018

Vet. Med. - Czech

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ABSTRACT:A four-year-old Bullmastiff weighing 44 kg was presented with a 14-day history of weight loss, vomiting and diarrhoea. Abdominal ultrasonography showed the presence of abdominal lymphadenopathy and thickening of the wall of the descending colon. Esophagogastroduodenoscopy and colonoscopy with biopsy were performed. Histological examination revealed a high-grade lymphoblastic lymphoma, flow cytometric analysis detected malignant cells of the immature B phenotype. PCR for antigen receptor rearrangement confirmed IgH monoclonality pointing together with immunophenotyping to B-cell lymphoma. The dog was treated using a multi-agent chemotherapy protocol. The overall survival time was 487 days. This was an unusual case of primary gastrointestinal B-lymphoblastic lymphoma in a dog with survival equivalent to that of the multicentric form.Keywords: canine; alimentary; lymphoid neoplasia; immunophenotyping; PARR; chemotherapy List of abbreviations:B-LBL = B-lymphoblastic lymphoma, FC = flow cytometry, FNAB = fine-needle aspiration biopsy, GI = gastrointestinal, LPE = lymphocytic-plasmacytic enteritis, PARR = polymerase chain reaction for antigen receptor rearrangement

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“…However, based on the current literature it can be stated that in most of the canine lymphoma cases this system of cytological classification is sufficient and evaluation of architectural pattern of growth, as in WR and WHO systems is not always necessary (Comazzi and Gelain 2011). Additionally, different therapeutic protocols in cases of different histologic subtypes of canine lymphoma in veterinary medicine are still not established and it seems that the updated Kiel classification system is sufficient enough in veterinary oncology Young 2007, Comazzi andGelain 2011).…”

Section: Discussionmentioning

confidence: 99%

High agreement of routine cytopathology and immunocytochemistry in canine lymphomas

Sapierzyński

Dolka²,

Fabisiak³

2012

Polish Journal of Veterinary Sciences

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The aim of this study was to compare the concordance of immunophenotype established with routine cytopathology (slides stained with Giemsa solution) and immunocytochemistry according to CD3 and CD79 alpha immunoreactivity. The study was performed on cytological samples of 70 canine lymphomas subtyped on the basis of the updated Kiel classification system. Additionally, cytologic samples were examined immunocytochemically for the CD3 and CD79 alpha antigens presence and thus immunophenotype of neoplastic growth was confirmed. The cytopathological and immunocytochemical diagnoses were then compared; in order to measure the concordance between immunocytochemistry (IC) result and Giemsa stain result of the same sample the Cohen's kappa coefficient was calculated. On the basis of the results of immunocytochemistry of 70 cases of canine lymphoma examined 42 were recognized as B cell lymphoma and 28 as T cell lymphoma. Full accordance between the results of routine cytopathology and IC was obtained in 63 out of 70 examined dogs (90% of cases). It can be concluded that cytopathological examination of Giemsa stained smears is helpful in determining the lymphoma cells immunophenotype. Additionally, it seems that combination of routine cytopathology and immunocytochemistry in cases of canine lymphomas allows to obtain the precise diagnosis in 90% of cases, and allows to receive most important information that is necessary for planning of appropriate therapy and to determine prognosis. Finally, this routine procedure allowed to eliminate the need of collection of tissue samples during surgery or core-biopsy and thus time, cost and patient discomfort related to more complex and invasive medical procedures can be easily reduced.

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Use of flow cytometric immunophenotyping to refine the cytological diagnosis of canine lymphoma

Cited by 66 publications

References 44 publications

Immunohistochemical Characterization of Canine Lymphomas

Immunohistochemical Characterization of Canine Lymphomas

Gastrointestinal B-lymphoblastic lymphoma in a dog: a case report

High agreement of routine cytopathology and immunocytochemistry in canine lymphomas

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