Use of fluorescent compounds in assessing bonded resin-based restorations: A literature review

D’Alpino, Paulo Henrique Perlatti; Pereira, José Carlos; Svizero, Nádia da Rocha; Rueggeberg, Frederick A.; Pashley, David H.

doi:10.1016/j.jdent.2005.12.004

Cited by 97 publications

(97 citation statements)

References 41 publications

(79 reference statements)

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“…The principal advantage of CLSM is that it allows the study of a volume of dentin in nondehydrated specimens at the subsurface level of specimens using histotomographic images. 16 The results of the present study showed that in all experimental groups the maximum depth of the sealer penetration was better in the coronal thirds than in the apical thirds of root canals. These results are in agreement with those of other studies.…”

supporting

confidence: 46%

Effects of Different Final Irrigating Solutions on Depth of Sealer Penetration into Dentinal Tubules: An in vitro Confocal Laser Microscopic Study

Shetty¹

2017

Journal of Operative Dentistry &Amp; Endodontics

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Aim: To evaluate the maximum depth of sealer penetration into dentinal tubules at the coronal, middle, and apical third of root canals, following the use of different final irrigation solutions. Materials and methods:Eighty freshly extracted mandibular premolars were selected for the study. After decoronation, instrumentation of samples was done using Protaper universal rotary file system till F4. During shaping and cleaning of root canal, 5.25% concentration of sodium hypochlorite (NaOCl) was used for irrigation. The samples were then randomly divided into four groups (n = 20) depending on the final irrigating solution used. Group I: 17% aqueous ethylenediaminetetraacetic acid (MA) (aq. EDTA); group II: 10% citric acid (CA); group III: 5% maleic acid; group IV: 5.25% conc. NaOCl. Passive ultrasonic irrigation (PUI) was used to activate each final irrigating solution. The samples were obturated using warm lateral condensation technique with guttapercha coated with AH plus sealer mixed with rhodamine B dye. Confocal laser scanning microscopy (CLSM) examined the sealer penetration depth. Results:The Kruskal-Wallis analysis results showed that there was a significant difference in the maximum depth of sealer penetration among all groups in all sections (p < 0.05). The coronal sections of the root canals in each group showed a significantly higher depth of sealer penetration than did the apical and middle sections (p < 0.05). Conclusion:A combination of 17% aq. EDTA and PUI used for final irrigation proved to be more effective for sealer penetration into the dentinal tubules.Clinical significance: Final irrigation protocol followed is of paramount importance in achieving complete disinfection within the root canal, and also the penetration of sealer serves as an indicator of the extent to which the smear layer was removed.

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supporting

confidence: 46%

Effects of Different Final Irrigating Solutions on Depth of Sealer Penetration into Dentinal Tubules: An in vitro Confocal Laser Microscopic Study

Shetty¹

2017

Journal of Operative Dentistry &Amp; Endodontics

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“…After placing the extruded sealer on a mixing pad, it was labeled with trace amount of fluorescent Rhodamine B dye (HiMediaLaboratories;India). [7] The dye-sealer mixture was placed along the entire length of the root canal with size 25 lentulo spiral keeping the instrument 2 mm from the canal apices with the hand piece running at 300 rpm. [8] In group UA, size 15 ultrasonic K-file (SatelecActeon, USA) was attached to a piezoelectric ultrasonic handpiece (SatelecActeon, USA) and used at medium power for the activation of the sealer.…”

Section: Methodsmentioning

confidence: 99%

Ultrasonic Activation of a Bioceramic Sealer and Its Dentinal Tubule Penetration: An In Vitro Study

Chadgal¹,

Farooq²,

Purra³

et al. 2018

AIMDR

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Background: The root canal sealers form an important component of the three dimensional obturation of the root canals. Moreover, the penetration of the sealers into the dentinal tubules is a desirable phenomenon for their adequate sealing and antibacterial action. The aim of this study was to evaluate the effects of ultrasonic activation on the intratubular penetration of bioceramic root canal sealer. Methods: Eighty extracted human mandibular premolars were divided into 2 groups (n =40) according to the sealer activation method used to obturate the root canals instrumented with F3 Pro-Taper instruments .The canals were obturated using Protaper F3 guttapercha cones. Previously, the bio ceramic sealer was labeled with rhodamine B dye to allow analysis under a confocal microscope. The two groups were: UA (ultrasonically activated) and NA (no activation; control). All samples were sectioned at 3 and 8 mm from the apex. The percentages of dentinal sealer penetration segments of canal were analyzed. Results: Students T test was performed for the statistical analysis and we found that there was a significant increase in tubular penetration for the ultrasonic activation group.Conclusion: Use of ultrasonic activation of a bioceramic sealer promoted greater dentinal sealer penetration.

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“…The minuscule molecular dimension of dyes allows penetration into inter-and intraprismatic micropores in enamel considered to be1-30 nm wide, as well as in the peri-or intertubular dentin having 0.8-2.5 µm wide dentinal tubules. The use of fluorescent compounds have expanded the possibilities to appraise microleakage "bidirectionally" [124], but the confounding variables remain as a methodological challenge.…”

Section: Microleakagementioning

confidence: 99%

Secondary caries and microleakage

Jokstad

2016

Dental Materials

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Objectives: To critically appraise experimental ex vivo research that has focussed on secondary caries, and to offer possible explanations for the seemingly poor correlation to clinical observations. Methods: The literature relating to the etiopathogenesis or prevention of secondary caries gained from experimental ex vivo research was reviewed, with particular emphasis on microleakage and artificial caries-like lesions.Results: It is doubtful whether a caries wall lesion can exist independently of an outer enamel caries lesion. Microleakage experiments apparently continue to emerge regardless of multiple reviews questioning the reliability and validity of the method. Several of the approaches used to generate artificial caries-like lesions are very aggressive. Remarkably little discussion has evolved about how these aggressive approaches create microenvironments that do not occur in reality. Corrosion-and biodegradation products may influence the biofilm qualitatively and quantitatively and it is difficult to replicate these variables in any ex vivo environment. Clinical data sampling method, patient demography as well as study methodology influences the incidence and prevalence estimates of secondary caries. Clinical results based on clinical work in settings where cost per unit time is of nominal concern do not provide any indications on how the restorative material will perform when placed by the average dentists in the mouths of their spectrum of patients during a busy workday.Recommendations: The term "wall lesion" including its variants is ill defined, has been, and is still being used indiscriminately. Stakeholders should avoid using this ambiguous label due to its connotation to an entity that does not exist per se.

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Use of fluorescent compounds in assessing bonded resin-based restorations: A literature review

Cited by 97 publications

References 41 publications

Effects of Different Final Irrigating Solutions on Depth of Sealer Penetration into Dentinal Tubules: An in vitro Confocal Laser Microscopic Study

Effects of Different Final Irrigating Solutions on Depth of Sealer Penetration into Dentinal Tubules: An in vitro Confocal Laser Microscopic Study

Ultrasonic Activation of a Bioceramic Sealer and Its Dentinal Tubule Penetration: An In Vitro Study

Secondary caries and microleakage

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