Use of fuzzy neural networks in modeling relationships of HPV infection with apoptotic and proliferation markers in potentially malignant oral lesions

Campisi, Giuseppina; Fede, Olga Di; Giovannelli, Lucia; Capra, Giuseppina; Greco, Immacolata; Calvino, Francesco Maria; Florena, Ada Maria; Muzio, Lorenzo Lo

doi:10.1016/j.oraloncology.2005.05.014

Cited by 11 publications

(11 citation statements)

References 72 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar to viral detection, the data regarding HPV typing available in the literature are also highly diverse. Some authors have found a higher prevalence of HPV 18, as also observed in the present investigation [9,15,28,29].…”

Section: Discussionsupporting

confidence: 91%

“…Similar to p53, no association was observed between the expression of bcl-2 and the presence of HPV in the oral carcinoma cases studied (t = 0.710; p = 0.482). These findings disagree with those reported by Badaracco et al [7], Rihakova et al [35], Grace et al [17], Du et al [18] and Campisi et al [28]. Probably, a high number of cases should be analyzed to clarify this matter.…”

Section: Discussioncontrasting

confidence: 70%

See 1 more Smart Citation

High-risk human papillomavirus (HPV) is not associated with p53 and bcl-2 expression in oral squamous cell carcinomas

et al. 2009

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 91%

Section: Discussioncontrasting

confidence: 70%

High-risk human papillomavirus (HPV) is not associated with p53 and bcl-2 expression in oral squamous cell carcinomas

et al. 2009

View full text Add to dashboard Cite

“…Resveratrol, curcumin, and epigallocatechin are among the many natural compounds that have displayed antiproliferative actions against cancer cells through the initiation of apoptosis. Therefore, a highly conserved 36 kDa nuclear protein of DNA polymerase-delta known as the PCNA has been identified as a useful marker that can be used to assess tumor cell proliferation and progression 68. An overexpression of PCNA has been reported in different malignancies, including breast cancer 69.…”

Section: Discussionmentioning

confidence: 99%

Involvement of NF-ΚB and HSP70 signaling pathways in the apoptosis of MDA-MB-231 cells induced by a prenylated xanthone compound, α-mangostin, from Cratoxylum arborescens

Ibrahim¹,

Hashim²,

Mohan³

et al. 2014

DDDT

View full text Add to dashboard Cite

BackgroundCratoxylum arborescens has been used traditionally in Malaysia for the treatment of various ailments.Methodsα-Mangostin (AM) was isolated from C. arborescens and its cell death mechanism was investigated. AM-induced cytotoxicity was observed with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Acridine orange/propidium iodide staining and annexin V were used to detect cells in early phases of apoptosis. High-content screening was used to observe the nuclear condensation, cell permeability, mitochondrial membrane potential, and cytochrome c release. The role of caspases-3/7, -8, and -9, reactive oxygen species, Bcl-2 and Bax expression, and cell cycle arrest were also investigated. To determine the role of the central apoptosis-related proteins, a protein array followed by immunoblot analysis was conducted. Moreover, the involvement of nuclear factor-kappa B (NF-κB) was also analyzed.ResultsApoptosis was confirmed by the apoptotic cells stained with annexin V and increase in chromatin condensation in nucleus. Treatment of cells with AM promoted cell death-transducing signals that reduced MMP by downregulation of Bcl-2 and upregulation of Bax, triggering cytochrome c release from the mitochondria to the cytosol. The released cytochrome c triggered the activation of caspase-9 followed by the executioner caspase-3/7 and then cleaved the PARP protein. Increase of caspase-8 showed the involvement of extrinsic pathway. AM treatment significantly arrested the cells at the S phase (P<0.05) concomitant with an increase in reactive oxygen species. The protein array and Western blotting demonstrated the expression of HSP70. Moreover, AM significantly blocked the induced translocation of NF-κB from cytoplasm to nucleus.ConclusionTogether, the results demonstrate that the AM isolated from C. arborescens inhibited the proliferation of MDA-MB-231 cells, leading to cell cycle arrest and programmed cell death, which was suggested to occur through both the extrinsic and intrinsic apoptosis pathways with involvement of the NF-κB and HSP70 signaling pathways.

show abstract

“…The risk of HR-HPV infection was found to be independently associated with survivin and PCNA expression, suggesting that these markers could be involved in HPV-mediated disorders of epithelial maturation [100]. Recently, Szarka et al reported an increase of HPV prevalence in OPMD with increasing malignant potential: 32.8%, 40.9% and 47.7% in Oral Lichen Planus, OL and OSCC respectively [101].…”

Section: Oral Benign Lesions Hpv-relatedmentioning

confidence: 99%

Oral HPV Infection: Current Strategies for Prevention and Therapy

Syrjänen

Termine

Capra³

et al. 2012

CPD

Self Cite

View full text Add to dashboard Cite

Infection with High Risk (HR) Human Papillomaviruses (HPVs) is the main aetiological agent of Cervical Squamous Cell Carcinoma (CSCC) and also associated in a subgroup of other neoplasms, including Oropharyngeal Squamous cell Carcinoma (OPSCC). HPV infection, in genital as in oral mucosa, can also be subclinical or associated with benign proliferative lesions (common warts, condylomas, papillomas) caused mostly by infection with Low Risk (LR)-HPVs. In the last decades, extensive research has resulted in growing knowledge on HPV biology and specifically viral life cycle, biochemical properties of viral proteins and their interaction with the host proteins leading to potential new targets of prophylactic or therapeutic vaccines and therapies for HPV infection. In addition, notable progresses have been made in the field of diagnostics to detect HPV DNA or RNA. The recent epidemiological data suggest the significant changes in HPV endemic, due to the changes in sexual habits especially among young generations (i.e. early sexual debuts, multiple sexual partners, oral and anal sex); this scenario has urged on the need of adequate campaigns of primary (sexual education, vaccination programs) and secondary prevention (diagnostics of HPV-related diseases). Due to the growing interest on HPV infection and HPV related cancers, the authors made a narrative review of the literature on oral HPV infection and oral-genital transmission. After this, in view of the controversies about the strategies of therapy and prevention of HPV infection, the present review focuses on the current state of art about the available tools for the therapeutic and, if any, preventive management of oral HPV infection.

show abstract

Use of fuzzy neural networks in modeling relationships of HPV infection with apoptotic and proliferation markers in potentially malignant oral lesions

Cited by 11 publications

References 72 publications

High-risk human papillomavirus (HPV) is not associated with p53 and bcl-2 expression in oral squamous cell carcinomas

High-risk human papillomavirus (HPV) is not associated with p53 and bcl-2 expression in oral squamous cell carcinomas

Involvement of NF-ΚB and HSP70 signaling pathways in the apoptosis of MDA-MB-231 cells induced by a prenylated xanthone compound, α-mangostin, from Cratoxylum arborescens

Oral HPV Infection: Current Strategies for Prevention and Therapy

Contact Info

Product

Resources

About

Use of fuzzy neural networks in modeling relationships of HPV infection with apoptotic and proliferation markers in potentially malignant oral lesions

Cited by 11 publications

References 72 publications

High-risk human papillomavirus (HPV) is not associated with p53 and bcl-2 expression in oral squamous cell carcinomas

High-risk human papillomavirus (HPV) is not associated with p53 and bcl-2 expression in oral squamous cell carcinomas

Involvement of NF-&Kappa;B and HSP70 signaling pathways in the apoptosis of MDA-MB-231 cells induced by a prenylated xanthone compound, &alpha;-mangostin, from Cratoxylum arborescens

Oral HPV Infection: Current Strategies for Prevention and Therapy

Contact Info

Product

Resources

About

Involvement of NF-ΚB and HSP70 signaling pathways in the apoptosis of MDA-MB-231 cells induced by a prenylated xanthone compound, α-mangostin, from Cratoxylum arborescens