Use of galantamine in the treatment of Alzheimer's disease and strategies to optimize its biosynthesis using the in vitro culture technique

Santos, Gabriella Sousa; Sinoti, Simone Batista Pires; Almeida, Francisco A. G.; Silveira, Dâmaris; Simeoni, Luiz Alberto; Gomes-Copeland, Kicia Karinne Pereira

doi:10.1007/s11240-020-01911-5

Cited by 19 publications

(4 citation statements)

References 138 publications

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“…Callus cultures have great potential for sustainable production of bioactive compounds of medicinal plants (Koufan et al 2020). However, undifferentiated calluses are not as promising as organogenesis cultures in terms of SMs production (Santos et al 2020). The superiority of differentiated cultures over the callus culture systems, in terms of level of produced bioactive compounds, has been reported in Schisandra henryi (Jafernik et al 2020), Phellodendron chinense (He et al 2020) and Argania spinosa (L.) (Koufan et al 2020).…”

Section: Discussionmentioning

confidence: 99%

Integrative effects of stress- and stress tolerance-inducing elicitors on in vitro bioactive compounds of ajowan [Trachyspermum ammi (L.) Sprague] medicinal plant

Niazian

Howyzeh

Sadat-Noori

2021

Plant Cell Tiss Organ Cult

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Bioactive compounds of medicinal plants have a wide range of applications in pharmaceutical, food and other industries. In vitro culture systems have great potential for sustainable production of bioactive compounds of medicinal plants. In the present study, the individual and combined effects of a stress tolerance-inducing (salicylic acid) and a stress-inducing elicitor (polyethylene glycol) were evaluated on regeneration e ciency, antioxidants activity and phytochemical pro le of in vitro shoot cultures of ajowan. Different concentrations of salicylic acid (SA) (0, 10, 20, 40, 80 µM) and polyethylene glycol (PEG 6000) (0, 1, 2, 5%) were added to the shoot regeneration Murashige and Skoog medium containing Kin (1.5 mg/L) and NAA (0.25 mg/L) plant growth regulators. Salicylic acid reduced the adverse effect of PEG treatment on number of regenerated shoots and in vitro rooting. The activities of catalase, superoxide dismutase, and peroxidase enzymatic antioxidants were signi cantly increased in SA + PEG treated plants. The gas chromatography-mass spectrometry (GC-MS)-pro ling revealed quantitative and qualitative phytochemical differences between control and SA + PEG treated plants. The greatest means of p-cymene and thymol bioactive compounds were obtained from in vitro shoots treated with 5% PEG + 40 µM SA. The inter-simple sequence repeats (ISSR) markers proved the genetic stability of in vitro regenerated plants. The presented protocol is useful for large-scale sustainable production of secondary metabolites (SMs) of medicinal plants. The same strategy (stress tolerance-inducing elicitor + stress-inducing elicitor) is applicable to increase valuable SMs in other production systems such as hydroponic, greenhouse and eld conditions.

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Section: Discussionmentioning

confidence: 99%

Integrative effects of stress- and stress tolerance-inducing elicitors on in vitro bioactive compounds of ajowan [Trachyspermum ammi (L.) Sprague] medicinal plant

Niazian

Howyzeh

Sadat-Noori

2021

Plant Cell Tiss Organ Cult

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“…Up to now, galanthamine production has mainly relied on natural resource exploitation from species such as Galanthus , Leucojum , Narcissus , etc. Providing galanthamine to the 55 million people living with dementia cannot solely rely on plant source, and in the case of some species like Leucojum , it has already endangered biodiversity of wild population in the past years [ 10 ]. As an alternative strategy, chemical synthesis of galanthamine has been attempted [ 11 , 12 ].…”

Section: Current Challenges In the Production Of Amaryllidaceae Alkal...mentioning

confidence: 99%

Biotechnological Approaches to Optimize the Production of Amaryllidaceae Alkaloids

et al. 2022

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Amaryllidaceae alkaloids (AAs) are plant specialized metabolites with therapeutic properties exclusively produced by the Amaryllidaceae plant family. The two most studied representatives of the family are galanthamine, an acetylcholinesterase inhibitor used as a treatment of Alzheimer’s disease, and lycorine, displaying potent in vitro and in vivo cytotoxic and antiviral properties. Unfortunately, the variable level of AAs’ production in planta restricts most of the pharmaceutical applications. Several biotechnological alternatives, such as in vitro culture or synthetic biology, are being developed to enhance the production and fulfil the increasing demand for these AAs plant-derived drugs. In this review, current biotechnological approaches to produce different types of bioactive AAs are discussed.

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“…Most importantly, the scale-up preparation of the colchicine precursor (S)-autumnaline with a high titer is achieved after replacing the rate-limiting O-methylation by the plug-and-play strategy. This study not only streamlines future engineering endeavors for colchicine biosynthesis, but also provides a paradigm for constructing more artificial biocatalytic cascades for the manufacture of diverse alkaloids through synthetic biology.Plant-derived tetrahydroisoquinoline (THIQ) alkaloids have widely been used to treat human diseases [1][2][3][4][5] . Based on their precursor scaffold structures 6 , they can be divided into benzylisoquinoline, phenethylisoquinoline (Fig.…”

mentioning

confidence: 99%

“…Plant-derived tetrahydroisoquinoline (THIQ) alkaloids have widely been used to treat human diseases [1][2][3][4][5] . Based on their precursor scaffold structures 6 , they can be divided into benzylisoquinoline, phenethylisoquinoline (Fig.…”

mentioning

confidence: 99%

Modular assembly of an artificially concise biocatalytic cascade for the manufacture of phenethylisoquinoline alkaloids

Gao,

Li,

Luo

et al. 2024

Nat Commun

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Plant-derived alkaloids are an important class of pharmaceuticals. However, they still rely on phytoextraction to meet their diverse market demands. Since multistep biocatalytic cascades have begun to revolutionize the manufacture of natural or unnatural products, to address the synthetic challenges of alkaloids, herein we establish an artificially concise four-enzyme biocatalytic cascade with avoiding plant-derived P450 modification for synthesizing phenethylisoquinoline alkaloids (PEIAs) after enzyme discovery and enzyme engineering. Efficient biosynthesis of diverse natural and unnatural PEIAs is realized from readily available substrates. Most importantly, the scale-up preparation of the colchicine precursor (S)-autumnaline with a high titer is achieved after replacing the rate-limiting O-methylation by the plug-and-play strategy. This study not only streamlines future engineering endeavors for colchicine biosynthesis, but also provides a paradigm for constructing more artificial biocatalytic cascades for the manufacture of diverse alkaloids through synthetic biology.

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Use of galantamine in the treatment of Alzheimer's disease and strategies to optimize its biosynthesis using the in vitro culture technique

Cited by 19 publications

References 138 publications

Integrative effects of stress- and stress tolerance-inducing elicitors on in vitro bioactive compounds of ajowan [Trachyspermum ammi (L.) Sprague] medicinal plant

Integrative effects of stress- and stress tolerance-inducing elicitors on in vitro bioactive compounds of ajowan [Trachyspermum ammi (L.) Sprague] medicinal plant

Biotechnological Approaches to Optimize the Production of Amaryllidaceae Alkaloids

Modular assembly of an artificially concise biocatalytic cascade for the manufacture of phenethylisoquinoline alkaloids

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