Use of gamma radiation to induce mutations in rice (Oryza sativa L.) and the selection of lines with tolerance to salinity and drought

Abdelnour-Esquivel, Ana; Pérez, Jason; Rojas, Miguel; Vargas, Walter M.; Gatica-Arias, Andrés

doi:10.1007/s11627-019-10015-5

Cited by 30 publications

(22 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The best radiation/regeneration ratio was achieved at 60 Gy with 83% regeneration, allowing a balance between gamma radiation at lethal dose 20 and regeneration (Table 4). The increased regeneration achieved with 40 Gy and 60 Gy versus the control is a hormetic behavior previously reported in our lab 13 . The potential of a temporary immersion system for regenerating difficult materials was also validated for the well-known recalcitrant cultivars CR1821 and CR1113 (Table 7, Fig.…”

Section: Resultssupporting

confidence: 83%

“…We believe 60 Gy is an excellent condition to start mutagenesis, considering that in other in vitro plants, such as pineapple, potato, and banana, a 5-40 Gy dose of gamma irradiation was shown to be sufficient to produce variability 24 . The calculated radiation dose of the cultivar at 60 Gy was the same as the LD50 for CR5272 13 .…”

Section: Discussionmentioning

confidence: 99%

“…Once irradiated, the seeds must be planted several times (M0 to M4) to avoid chimeras and heterogenicity until exposed to stress selection conditions, as has been done in the past6,8. In contrast, rice tissue culture can produce a primitive cell aggregate or callus with embryogenic potential and consequently mutate and regenerate from one or a few cells with stressor selection from the beginning 12,13 .…”

Section: Introductionmentioning

confidence: 99%

“…The latter is possible because plant cells are totipotent, which means that whole plants can develop from the single cells 14 . Mutation in the embryogenic callus using gamma radiation was previously developed in our lab for the cultivar CR5272 for salt tolerance; however, farmers no longer use this cultivar and instead use modern materials that are more recalcitrant to tissue culture 13 . The establishment of embryogenic rice calli is influenced by the germplasm of origin and the 2,4-D concentration 14,15 .…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Temporary Immersion System Improves Regeneration of in vitro Irradiated Recalcitrant Rice Embryogenic Calli

Hernández-Soto¹,

Pérez-Chávez²,

Fait-Zúñiga³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

The development of gamma rays mutant rice lines would be a solution for introducing variability in already farmer using varieties. In vitro gamma (60Co) mutagenesis reduces chimeras and allows a faster selection of desired traits but requires laboratory process optimization. The objective of the present work was the in vitro establishment of a recalcitrant rice embryogenic calli, the determination of its sensitivity to gamma radiation (Co-60), sequencing MATK and Rubisco for identification purposes, as well as generation optimization. The radiosensitivity of embryogenic calli resulted in an LD50 of 110Gy, while the 20% lethal dose was 64Gy. All sequenced genes matched perfectly with already reported MATK and Rubisco O. sativa genes with a clear SNP that identifies the local variety related to the southeast Asia Region. Callus induction improves with an MS with 2mg/L 2,4D, and the regeneration was achieved with an MS medium with 3mg/L BAP and 0,5mg/L NAA. The optimized radiation condition was 60Gy with an 83% regeneration in a semisolid medium, allowing a balance between mutation and regeneration. When increased to 80Gy, the regeneration rate falls to 29%. An immersion system (RITA®) of either 60 or 120 seconds every 8hours allowed a systematic and homogeneous total regeneration of the recalcitrant line, in contrast with the semisolid medium that resulted in positive but irregular regeneration. Other well-known recalcitrant cultivars, CR1821, CR1113 also had an improving regeneration in the immersion system, demonstrating its potential use for recalcitrant materials. To our knowledge, this is the first report on using an immersion system to allow regeneration of gamma-ray mutants from recalcitrant rice materials.

show abstract

Section: Resultssupporting

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Temporary Immersion System Improves Regeneration of in vitro Irradiated Recalcitrant Rice Embryogenic Calli

Hernández-Soto¹,

Pérez-Chávez²,

Fait-Zúñiga³

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…The selection of in vitro obtained germoplasm continue to receive attention even the genetic modification in plants gained today a huge interest mostly related to clustered regularly interspaced short palindromic repeats (CRISPR) technology [18,19]. In vitro multiplication continues to be an essential part for the entire technological flow in plants breeding [20][21][22]. On the other hand, the continuous in vitro plant's experimentation may reveal new insights related to the connectivity between drought tolerance and morphogenesis.…”

Section: Introductionmentioning

confidence: 99%

Drought Stress Study on Nicotiana tabacum L., “Baladi”, an In Vitro Experimental Model

Antofie

Sand

2021

Agriculture

View full text Add to dashboard Cite

Crops drought tolerance is a trait of outmost importance for agriculture especially today when climate change is affecting more the production for food and feed. The scope of this article is to evaluate in vitro drought stress response of Nicotiana tabacum L., “Baladi”. The experiment was set up for four successive stages starting with in vitro seedling development, hypocotyl cultivation, three generations of micropropagation, pre-acclimatization and acclimatization. The effect of abscisic acid (ABA) and/or polyethylene-glycol 6000 (PEG) on tobacco hypocotyl caulogenesis and micropropagation were investigated. Superoxide-dismutases (SODs) and peroxidases (POXs) are more active and different isoforms patterns have been identified compared to the control for cualogenesis. A decrease of internodes length and a higher shoots multiplication rate were observed. However, under PEG treatment plantlets expressed hyperhydration and ceased rooting. Pre-treatments effects study of ABA and/or PEG were finalized in acclimatization phase for 18 tobacco clones. A summary of our results revealed that ABA and/or PEG induce among others a higher oxidative stress compared to the control in the first stage that is not maintained for all clones until acclimatization. Certain clones expressed a lower SOD activity compared to the control during acclimatization but maintaining higher POX activity.

show abstract

Mechanical and radiation shielding properties of SWCNT reinforced polymer/glass fiber fabric‐based nanocomposite containing different filler materials: A comparative study

Sürücü

Subaşı

Danish

et al. 2022

J of Applied Polymer Sci

View full text Add to dashboard Cite

In this study, polymer/glass fiber fabric‐based nanocomposite plates were fabricated with 0.01%–0.1% single‐walled carbon nanotubes (SWCNTs) and filler materials (barite, magnetite, and colemanite) using a hand layup process. Mechanical properties (e.g., tensile strength, flexural strength, and Charpy impact strength), thermal conductivity, and radiation shielding properties (e.g., gamma radiation and neutron radiation) of the specimens were determined. The results revealed that the specimens containing barite and magnetite managed to exhibit adequate mechanical properties (especially Charpy impact strength). Among different filler materials used, barite‐filled specimens outperformed colemanite and magnetite‐filled specimens in terms of mechanical properties. The mechanical performance of filler‐modified specimens can be further enhanced by adopting efficient dispersion techniques to disperse filler material and SWCNTs throughout the composite plates. The thermal conductivity of barite, magnetite, and colemanite‐filled specimens (with/without SWCNTs) increased by 30.56%–60% as compared to specimens only containing SWCNTs and neat polymer, which avoids the accumulation of heat required for radiation shielding applications. Similar to thermal conductivity, specimens containing filler materials (with and without SWCNTs) provided higher gamma and neutron radiation shielding properties as compared to neat polymer‐ and SWCNT‐modified specimens. In the case of gamma and neutron radiation shielding, barite‐ and colemanite‐filled specimens provided better results, respectively.

show abstract

Use of gamma radiation to induce mutations in rice (Oryza sativa L.) and the selection of lines with tolerance to salinity and drought

Cited by 30 publications

References 29 publications

Temporary Immersion System Improves Regeneration of in vitro Irradiated Recalcitrant Rice Embryogenic Calli

Temporary Immersion System Improves Regeneration of in vitro Irradiated Recalcitrant Rice Embryogenic Calli

Drought Stress Study on Nicotiana tabacum L., “Baladi”, an In Vitro Experimental Model

Mechanical and radiation shielding properties of SWCNT reinforced polymer/glass fiber fabric‐based nanocomposite containing different filler materials: A comparative study

Contact Info

Product

Resources

About