Use of Gelatin as Tannic Acid Carrier for Its Sustained Local Delivery

doi:10.20900/pf20200002

Cited by 4 publications

(6 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…COL/TA films showed no cytotoxicity independently of the ending layer. Noteworthily, the concentration of TA released (1 µM) from these films was less than its reported cytotoxic concentration (125 µM) towards NIH3T3 mouse fibroblasts [ 45 ]. At low concentrations, TA was reported to improve cell adhesion [ 46 ] and differentiation [ 36 ].…”

Section: Resultsmentioning

confidence: 99%

Physical Chemistry Study of Collagen-Based Multilayer Films

Chen

Iqbal

Meyer

et al. 2023

Gels

View full text Add to dashboard Cite

The surface properties of a biomaterial play an important role in cell behavior, e.g., recolonization, proliferation, and migration. Collagen is known to favor wound healing. In this study, collagen (COL)-based layer-by-layer (LbL) films were built using different macromolecules as a partner, i.e., tannic acid (TA), a natural polyphenol known to establish hydrogen bonds with protein, heparin (HEP), an anionic polysaccharide, and poly(sodium 4-styrene sulfonate) (PSS), an anionic synthetic polyelectrolyte. To cover the whole surface of the substrate with a minimal number of deposition steps, several parameters of the film buildup were optimized, such as the pH value of the solutions, the dipping time, and the salt (sodium chloride) concentration. The morphology of the films was characterized by atomic force microscopy. Built at an acidic pH, the stability of COL-based LbL films was studied when in contact with a physiological medium as well as the TA release from COL/TA films. In contrast to COL/PSS and COL/HEP LbL films, COL/TA films showed a good proliferation of human fibroblasts. These results validate the choice of TA and COL as components of LbL films for biomedical coatings.

show abstract

Section: Resultsmentioning

confidence: 99%

Physical Chemistry Study of Collagen-Based Multilayer Films

Chen

Iqbal

Meyer

et al. 2023

Gels

View full text Add to dashboard Cite

show abstract

“…TA/COL films switch significantly from biocompatible to cytotoxic toward human myoblasts depending on the buildup method. TA based LbL films were shown to be stable in the physiological medium with a part of TA released. − We then monitored the TA release of both TA/COL films. Upon incubation in PBS at room temperature, the release of TA followed an initial linear kinetic with roughly 86% of release within the first 4 h, which then stabilized over 1–4 days.…”

Section: Resultsmentioning

confidence: 99%

“…It was shown in vitro that polyphenol-rich plum extract treatment enhanced the size of C2C12 mouse myoblasts and accelerated their differentiation . Previously, we reported the use of tannic acid (TA) to develop hydrogen-bonded layer-by-layer (LbL) nanocoatings of COL type I using the dipping method. , Hydrogen-bonded TA LbL coatings with neutral polymers offer tunable stability over a wide pH range, thanks to the p K a of TA . In 2018, Park et al introduced the brushing method, using a paintbrush, to functionalize a surface with chitosan/alginate LbL films for drug delivery; however, no alignment of macromolecules was investigated .…”

Section: Introductionmentioning

confidence: 99%

Brush-Induced Orientation of Collagen Fibers in Layer-by-Layer Nanofilms: A Simple Method for the Development of Human Muscle Fibers

et al. 2022

View full text Add to dashboard Cite

The engineering of skeletal muscle tissue, a highly organized structure of myotubes, is promising for the treatment of muscle injuries and muscle diseases, for replacement, or for pharmacology research. Muscle tissue development involves differentiation of myoblasts into myotubes with parallel orientation, to ultimately form aligned myofibers, which is challenging to achieve on flat surfaces. In this work, we designed hydrogen-bonded tannic acid/collagen layer-by-layer (TA/COL LbL) nanofilms using a simple brushing method to address this issue. In comparison to films obtained by dipping, brushed TA/COL films showed oriented COL fibers of 60 nm diameter along the brushing direction. Built at acidic pH due to COL solubility, TA/COL films released TA in physiological conditions with a minor loss of thickness. After characterization of COL fibers’ orientation, human myoblasts (C25CL48) were seeded on the oriented TA/COL film, ended by COL. After 12 days in a differentiation medium without any other supplement, human myoblasts were able to align on brushed TA/COL films and to differentiate into long aligned myotubes (from hundreds of μm up to 1.7 mm length) thanks to two distinct properties: (i) the orientation of COL fibers guiding myoblasts’ alignment and (ii) the TA release favoring the differentiation. This simple and potent brushing process allows the development of anisotropic tissues in vitro which can be used for studies of drug discovery and screening or the replacement of damaged tissue.

show abstract

“…The antimicrobial activity of TA on E. coli and S. aureus and the minimum inhibitory concentration (MIC), already confirmed in a previous work, were determined at 105 μg/mL for E. coli and 100 μg/mL for S. aureus. 41 However, TA can also be cytotoxic for cells over a concentration of 200 μg/mL. 41 Quantification of TA released from the cone after several days is mandatory to predict its effects against bacteria and its toxicity.…”

Section: Discussionmentioning

confidence: 99%

“…41 However, TA can also be cytotoxic for cells over a concentration of 200 μg/mL. 41 Quantification of TA released from the cone after several days is mandatory to predict its effects against bacteria and its toxicity. The results showed that the concentration of TA released from the cones was 58 μg/mL (60% of the total concentration detected) after 24 h and 97 μg/mL after 10 days.…”

Section: Discussionmentioning

confidence: 99%

Highly Structured 3D Electrospun Conical Scaffold: A Tool for Dental Pulp Regeneration

Terranova

Louvrier

Hébraud

et al. 2021

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

New procedures envisioned for dental pulp regeneration after pulpectomy include cell homing strategy. It involves host endogenous stem cell recruitment and activation. To meet this cell-free approach, we need to design a relevant scaffold to support cell migration from tissues surrounding the dental root canal. A composite membrane made of electrospun poly(lactic acid) nanofibers and electrosprayed polycaprolactone with tannic acid (TA) microparticles which mimics the architecture of the extracellular matrix was first fabricated. After rolling the membrane in the form of a 3D conical scaffold and subsequently coating it with gelatin, it can be directly inserted into the root canal. The porous morphology of the construct was characterized by SEM at different length scales. It was shown that TA was released from the 3D conical scaffold after 2 days in PBS at 37 °C. Biocompatibility studies were first assessed by seeding human dental pulp stem cells (DPSCs) on planar membranes coated or not coated with gelatin to compare the surfaces. After 24 h, the results highlighted that the gelatin-coating increased the membrane biocompatibility and cell viability. Similar DPSC morphology and proliferation on both membrane surfaces were observed. The culture of DPSCs on conical scaffolds showed cell colonization in the whole cone volume, proving that the architecture of the conical scaffold was suitable for cell migration.

show abstract

Use of Gelatin as Tannic Acid Carrier for Its Sustained Local Delivery

Cited by 4 publications

References 25 publications

Physical Chemistry Study of Collagen-Based Multilayer Films

Physical Chemistry Study of Collagen-Based Multilayer Films

Brush-Induced Orientation of Collagen Fibers in Layer-by-Layer Nanofilms: A Simple Method for the Development of Human Muscle Fibers

Highly Structured 3D Electrospun Conical Scaffold: A Tool for Dental Pulp Regeneration

Contact Info

Product

Resources

About