Use of gelatin/plasma coated flasks for isolating human peripheral blood monocytes

Freundlich, Bruce; Avdalović, Nebojša

doi:10.1016/0022-1759(83)90107-2

Cited by 208 publications

(74 citation statements)

References 8 publications

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“…Leukocytes were harvested from heparinized venous blood (20), diluted 1 : 1 with calcium-and magnesium-poor sterile phosphate buffered saline, and fractionated with Ficoll-Hypaque to separate mononuclear from polymorphonuclear cells. PBM were obtained by adherence to gelatin-coated flasks (21). Nonadherent cells were washed off the flasks, and PBM were removed from the flasks with 5 mM EDTA.…”

Section: Methodsmentioning

confidence: 99%

Alteration of the cellular fatty acid profile and the production of eicosanoids in human monocytes by gamma‐linolenic acid

Pullman-Mooar

Laposata

Lem

et al. 1990

Arthritis & Rheumatism

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We administered borage seed oil (9 capsules/day) for 12 weeks to 7 normal controls and to 7 patients with active rheumatoid arthritis. The therapy provided 1.1 gm/day of y-linolenic acid (GLA). GLA administration resulted in increased proportions of its first metabolite, dihomo-y-linolenic acid (DGLA), in circulating mononuclear cells. The ratios of DGLA to arachidonic acid and DGLA to stearic acid increased significantly in these cells. Significant reductions in prostaglandin E,, leukotriene B,, and leukotriene C, produced by stimulated monocytes were seen after 12 weeks of GLA supplementation. The antiintlammatory effects of GLA administration observed in animal models, and the apparent clinical improvement experienced by 6 of 7 rheumatoid arthritis patients given borage seed oil in this open,

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Section: Methodsmentioning

confidence: 99%

Alteration of the cellular fatty acid profile and the production of eicosanoids in human monocytes by gamma‐linolenic acid

Pullman-Mooar

Laposata

Lem

et al. 1990

Arthritis & Rheumatism

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show abstract

“…Monocytes were puri®ed by density gradient centrifugation and the differential adherence procedure described by Freundlich and Avdalovic (1983). The heparinized blood was centrifuged at 150´g, and the plasma layer was collected and recentrifuged at 1300´g to pellet the platelets.…”

Section: Preparation and Culture Of Human Monocyte-derived Macrophagesmentioning

confidence: 99%

The Salmonella virulence plasmid spv genes are required for cytopathology in human monocyte-derived macrophages

et al. 2000

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SummaryThe pathogenesis of serious systemic Salmonella infections is characterized by survival and proliferation of bacteria inside macrophages. Infection of human monocyte-derived macrophages in vitro with S. typhimurium or S. dublin produces cytopathology characterized by detachment of cells that contain large numbers of proliferating bacteria. This cytopathology is dependent on the expression of the bacterial spv genes, a virulence locus previously shown to markedly enhance the ability of Salmonella to produce systemic disease. After 24 h of infection, macrophage cultures contain two populations of bacteria: (i) proliferating organisms present in a detached cell fraction; and (ii) a static bacterial population in macrophages remaining attached to the culture well. Mutations in either the essential transcriptional activator SpvR or the key SpvB protein markedly reduce the cytopathic effect of Salmonella infection. The spvdependent cytopathology in macrophages exhibits characteristics of apoptosis, with release of nucleosomes into the cytoplasm, nuclear condensation and DNA fragmentation. The current ®ndings suggest that the mechanism of the spv effect is through induction of increased cytopathology in host macrophages.

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“…However, unlike progenitor cells they display low proliferative capacity, cannot form EC tube-like structures in an in vitro angiogenesis assay model, but do display panleukocyte and monocytic/macrophage markers, such as CD14 and CD45 (Rehman et al, 2003;Zhang et al, 2006Zhang et al, , 2007. In support of this finding, previous data showed that monocytes have high affinity for fibronectin, and that 90% of cells from PB samples which adhere to coated dishes are of monocytic origin (Freundlich & Avdalovic, 1983). Moreover, a recent study by Prokopi et al suggested how these putative EPCs may acquire EC markers.…”

Section: Epcs Phenotypic and Functional Propertiesmentioning

confidence: 61%

Vasculogenesis in Diabetes-Associated Diseases: Unraveling the Diabetic Paradox

Costa¹

2011

Vasculogenesis and Angiogenesis - From Embryonic Development to Regenerative Medicine

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Use of gelatin/plasma coated flasks for isolating human peripheral blood monocytes

Cited by 208 publications

References 8 publications

Alteration of the cellular fatty acid profile and the production of eicosanoids in human monocytes by gamma‐linolenic acid

Alteration of the cellular fatty acid profile and the production of eicosanoids in human monocytes by gamma‐linolenic acid

The Salmonella virulence plasmid spv genes are required for cytopathology in human monocyte-derived macrophages

Vasculogenesis in Diabetes-Associated Diseases: Unraveling the Diabetic Paradox

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