Use of graphitised carbon negative ion LC–MS to analyse enzymatically digested glycosaminoglycans

Karlsson, Niclas G.; Schulz, Benjamin L.; Packer, Nicolle H.; Whitelock, John M.

doi:10.1016/j.jchromb.2005.07.014

Cited by 65 publications

(54 citation statements)

References 33 publications

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“…Porous graphitized carbon LC/MS has been applied to GAGs (51,52). This approach appears most suited to the analysis of GAGs, such as CS, containing no more than one sulfate group per disaccharide unit.…”

Section: The Analytical Challengementioning

confidence: 99%

Glycosaminoglycan Glycomics Using Mass Spectrometry

Zaia

2013

Molecular & Cellular Proteomics

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The fact that sulfated glycosaminoglycans (GAGs) are necessary for the functioning of all animal physiological systems drives the need to understand their biology. This understanding is limited, however, by the heterogeneous nature of GAG chains and their dynamic spatial and temporal expression patterns. GAGs have a regulated structure overlaid by heterogeneity but lack the detail necessary to build structure/function relationships. In order to provide this information, we need glycomics platforms that are sensitive, robust, high throughput, and information rich. This review summarizes progress on massspectrometry-based GAG glycomics methods. The areas covered include disaccharide analysis, oligosaccharide profiling, and tandem mass spectrometric sequencing.

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Section: The Analytical Challengementioning

confidence: 99%

Glycosaminoglycan Glycomics Using Mass Spectrometry

Zaia

2013

Molecular & Cellular Proteomics

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“…Furthermore, electrospray ionization (ESI) is known to be suitable for analysis of polar compounds. Thus, ESI-MS/MS has been used to analyze sequencing KSrelated oligosaccharides (Oguma et al, 2001;Karlsson et al, 2005;Zhang et al, 2005a;Zhang et al, 2005b;Minamisawa et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Analytical method for determination of disaccharides derived from keratan sulfates in human serum and plasma by high‐performance liquid chromatography/turbo‐ionspray ionization tandem mass spectrometry

Oguma

Tomatsu

Okazaki

2007

Biomedical Chromatography

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We established a highly sensitive LC/MS/MS method for the analysis of the disaccharides produced from keratan sulfates (KS). It was revealed that the disaccharides produced by keratanase II enzymatic digestion of KS could be determined with high sensitivity by negative ion mode of multiple reaction monitoring. Furthermore, monosulfated and disulfated disaccharides can be separated using a Hypercarb (2.0 mm i.d. x 150 mm, 5 microm) with a gradient elution of acetonitrile-0.01 m ammonium bicarbonate (pH 10). This method was applied to the determination of KS in serum and plasma of control subjects. The intra-day precision expressed as %CV was within 6.8% for five replicate analyses with three different control serum. The inter-day (overall, n = 15) precision was within 7.3% for three days. This method is sensitive, reproducible and would be useful for clinical analysis.

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“…This spectral pattern was highly similar to the previously reported one for HA tetrasaccharide. 31) In the spectra, a fragment ion with m/z below the low mass cut-o# value (in this case, ca. m/z 215) can, in principle, never be observed.…”

Section: Oh]mentioning

confidence: 99%

Characterization of Isomeric Unsulfated Glycosaminoglycan Oligosaccharides by Mass Spectrometry/Mass Spectrometry

Minamisawa

Suzuki

Maéda

et al. 2007

J. Mass Spectrom. Soc. Jpn.

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Both saturated and unsaturated forms of isomeric unsulfated glycosaminoglycan (GAG) oligosaccharides, i.e., tetrasaccharides of chondroitin (CH) and hyaluronan (HA), were analyzed by electrospray ionization mass spectrometry/mass spectrometry. Although the only structural di#erence between them was the hydroxyl group at the C-4 position in N-acetylhexosamine (GalNAc or GlcNAc, respectively), given the same m/z value of precursor ions, these isomers in both their saturated and unsaturated forms could be separated by careful examination of diagnostic fragment ions in their product ion mass spectra when the relative abundances of these fragment ions were considered. In addition, the product ion mass spectrum of the unsaturated HA tetrasaccharide was compared with its linkage isomer, N-acetylheparosan tetrasaccharide. In this case, the isomers were more easily di#erentiated by comparing their characteristic spectral patterns. By adopting this approach, systematic di#erentiation of isomeric unsulfated GAG oligosaccharides should be achieved by means of fragmentation. It should also contribute widely to GAG-related biochemical and medicinal research in the future.

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Use of graphitised carbon negative ion LC–MS to analyse enzymatically digested glycosaminoglycans

Cited by 65 publications

References 33 publications

Glycosaminoglycan Glycomics Using Mass Spectrometry

Glycosaminoglycan Glycomics Using Mass Spectrometry

Analytical method for determination of disaccharides derived from keratan sulfates in human serum and plasma by high‐performance liquid chromatography/turbo‐ionspray ionization tandem mass spectrometry

Characterization of Isomeric Unsulfated Glycosaminoglycan Oligosaccharides by Mass Spectrometry/Mass Spectrometry

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