Use of hair root as a source of DNA for the detection of heterozygotes for recessive defects in cattle

Pj, Healy; Ja, Dennis; Jf, Moule

doi:10.1111/j.1751-0813.1995.tb06178.x

Cited by 17 publications

(10 citation statements)

References 10 publications

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“…High quality genomic DNA was extracted from semen using a phenol-chlorophorm method (Sambrook et al, 1989) and from blood and hair roots using the method described by Aljanabi and Martinez, (1997). DNA was also extracted from hair roots using a rapid, low cost method (Healy et al, 1995) suitable for routine microsatellite genotyping but producing low quality DNA.…”

Section: Samplesmentioning

confidence: 99%

Real-time PCR genotyping and frequency of the myostatin F94L mutation in beef cattle breeds

Vankan

Waine

Fortes

2010

Animal

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This research developed two real-time PCR assays, employing high-resolution melt and allele-specific analysis to accurately genotype the F94L mutation in cattle. This mutation (g.433C . A) in the growth differentiation factor 8 or myostatin gene has recently been shown to be functionally associated with increased muscle mass and carcass yield in cattle. The F94L mutation is not, like other myostatin mutations, associated with reduced fertility and dystocia. It is therefore a candidate for introgression into other breeds to improve retail beef yield and the development of a simple and accurate test to genotype this specific mutation is warranted. Variations in the efficiency of enzyme cleavage compromised the accuracy of genotyping by published methods, potentially resulting in an overestimation of the frequency of the mutant allele. The frequency of the F94L mutation was determined by real-time PCR in 1140 animals from 15 breeds of cattle in Australia. The mutation was present in Simmental (0.8%), Piedmontese (2%), Droughtmaster (4%) and Limousin (94.2%) but not found in Salers, Angus, Poll Hereford, Hereford, Gelbvieh, Charolais, Jersey, Brahman, Holstein, Shorthorn or Maine Anjou. The low prevalence of F94L in all beef breeds except Limousin indicates the significant potential for this mutation to improve retail yield in Australian beef cattle.

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Section: Samplesmentioning

confidence: 99%

Real-time PCR genotyping and frequency of the myostatin F94L mutation in beef cattle breeds

Vankan

Waine

Fortes

2010

Animal

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“…DNA was extracted from hair roots (Healy et al, 1995), liophilyzed bloods and muscle samples (Extraction kit, Quiagen, Italy). DNA samples of different breeds (Table S1) were used to sequence ADIPOQ regions.…”

Section: Sampling and Dna Extractionmentioning

confidence: 99%

Study of porcine adiponectin (ADIPOQ) gene and association of a missense mutation with EBVs for production and carcass traits in Italian Duroc heavy pigs

Dall’Olio

Davoli

Buttazzoni³

et al. 2009

Livestock Science

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“…Hair roots in anagen, preferably with sheaths attached, are required for assays. Hair roots in telogen lack nucleated cells and cannot serve as a useful source of genomic DNA (Healy et al, 1995). The collection, transportation and storage of hair bulbs do not require any special conditions and any financial costs in contrast to other tissues such as blood.…”

mentioning

confidence: 99%

Implications for the use of horse hair roots as a DNA source for microsatellite typing

Ząbek¹,

Radko²,

Słota³

2005

CZECH J ANIM SCI

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Hair roots are a very attractive source of DNA for microsatellite-based parentage control of breeding animals. However, unlike blood samples, irregular DNA typing results have been observed in assays utilizing hair follicles. The amount of starting material and DNA preparation method are the crucial factors. In order to improve DNA typing results for horse hair roots, two quick preparation methods and additional purification steps were evaluated. PCR efficiency for each approach was expressed as percentage of samples with complete DNA profiles for 12 horse microsatellites. The lowest percentage (22%) of complete DNA profiles was obtained for samples prepared by the proteinase K digestion method. The best genotyping results (94%) were achieved after phenolchloroform extraction of DNA from samples prepared by the proteinase K digestion method. Direct cleanup of DNA samples with an ethanol-sodium acetate mixture gave comparably good results of microsatellite genotyping (91%). DNA preparation from hair roots with proteinase K digestion followed by DNA purification with ethanol was chosen as the most efficient approach for horse DNA typing under parentage testing.

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Use of hair root as a source of DNA for the detection of heterozygotes for recessive defects in cattle

Cited by 17 publications

References 10 publications

Real-time PCR genotyping and frequency of the myostatin F94L mutation in beef cattle breeds

Real-time PCR genotyping and frequency of the myostatin F94L mutation in beef cattle breeds

Study of porcine adiponectin (ADIPOQ) gene and association of a missense mutation with EBVs for production and carcass traits in Italian Duroc heavy pigs

Implications for the use of horse hair roots as a DNA source for microsatellite typing

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